LOCUS       sequence-339296-        3267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a 
            pcDNA3.1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Berlew EE, Yamada K, Kuznetsov IA, Rand EA, Ochs CC, Jaber Z, 
            Gardner KH, Chow BY
  TITLE     Designing Single-Component Optogenetic Membrane Recruitment Systems:
            The Rho-Family GTPase Signaling Toolbox.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):515-521. doi: 
            10.1021/acssynbio.1c00604. Epub 2022 Jan 3.
  PUBMED    34978789
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00604"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "515-521"
COMMENT     Sequence Label: opto-Tiam1
FEATURES             Location/Qualifiers
     source          1..3267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2554..3264
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2699..2717)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2967..2986
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggccacag acgcaatcga ccattactac cagaacagag acggcccccc ttccaggagc
       61 tttagcgacg aatacgactc atacaataag acagcccctc tgccacctct gagcgcctgc
      121 ccagagcgcg cccagacccc taacagcaag agaatcccca tcacacccaa ttccgccaag
      181 agcacctccg agtctcctcg gagaatcaca ttctctcgcg acgcaggcag ctccaccaag
      241 ggcagcaccc agacaagccc atccaaccag ggctccgtgt ctagccggaa tggctctaac
      301 ggaaatggcc tgggcctgtc ctctgccggc aacttcgccg atttctttag ctccgaggtg
      361 ttttatatcg tgctgcacaa tcccaccaca gcccacagat tcctgaagtt ttgccagagc
      421 agggcctgtt ccgagaacct ggagtttctg cagaagatcg acgagtacaa taggctgatc
      481 gatgaggcca cacgcctgct gtctaccatc tacagcacat acgtgagcag cgaggcccct
      541 aagcagatca acatctctaa tagccacgcc aggcgcctga gccacgacat caagggagca
      601 acccacaacg tgctgcccgg cctggaggat atcttcaatg gctcccagga gcagatcgag
      661 aagctgctgg cctctgacct gtatcctaga tttgtgaagc accaggtgac cgcatccgcc
      721 acaatggccc tggcaaacga caaggagagg tacccaggcc tgggcgattg cttctgtctg
      781 acagacccac ggagagccga taatcccatc gtgttcgcaa gcgacggatt cgtgagcgtg
      841 accggctatt cccgctctga tatcatccca aggaactgcc gcttcctgca gggcagcttt
      901 accgaccggc aggccacaaa gaggctgcgc acctccatcg agaatcgcga ggagacagtg
      961 gagctgctgc tgaactatcg gaagaatggc gatccctttt ggaacctgct gtacgtgtcc
     1021 cctctgctgg acggcaatgg cgatgtgaga ttctttctgg gcggccagat caactgttct
     1081 accacaatcc acagccggac cgacgtgctg agaatcctga gcctgaacga cgaggataca
     1141 ggctccttca tcgagggctc taataagacc cccagcgtgc ggtccaagga gtctaacaat
     1201 gcctccaact atggcaagac atccttcttt aagtctttta agaagtacaa ggccaatacc
     1261 gtggagatca gagacgaggc cggcatggag ggcgagctga tcgataggat cggcaagctg
     1321 cagttcaaga cacaggtgga ggagttttac accgcctatt ctaagtacct ggtgctgagc
     1381 tatcagccac agtcccagaa cctgatcgtg aagtacttca gccccggcat cgtggatatg
     1441 ctgaacctga atatgcctaa tggcctggtg gccccaatcg tggacaagga tatcttcaag
     1501 gtgctgacag agcactcccc ttcctctgtg ccacggacct ttaagaacgt ggtgagggaa
     1561 ggcatcaaag caggcagggc cgtgtctgtg gagacaggcc tgctgacagg catcgaggag
     1621 atcaagaagt ctcagaacat cttcagcggc accacagcca atcgggagag aggctggaag
     1681 aaggccgagg agaagtacgt gtgccacttt accccctgta aggatgagat gggcagggtc
     1741 ggctgggtgg tgctgactgt cgctcctaaa ctggaaagat ttgaattcgg tggcggatca
     1801 ggaggagggt caatgaaccc ctctgaccag agcccatctc ctcaggactc cacggggcct
     1861 cagctggcga ccatgagaca actctcggat gcagataagc tgcgcaaggt gatctgcgag
     1921 ctcctggaga cggagcgcac ctacgtgaag gatttaaact gtcttatgga gagataccta
     1981 aagcctcttc aaaaagaaac ttttctcacc caggatgagc ttgacgtgct ttttggaaat
     2041 ttaacggaaa tggtagagtt tcaagtagaa ttccttaaaa ctctagaaga tggagtgaga
     2101 ctggtacctg atttggaaaa gcttgagaag gttgatcaat ttaagaaagt gctgttctct
     2161 ctggggggat cattcctgta ttatgctgac cgcttcaagc tctacagtgc cttctgcgcc
     2221 agccacacaa aagttcccaa ggtcctggtg aaagccaaga cagacacggc tttcaaggca
     2281 ttcttggatg cccagaaccc gaagcagcag cactcatcca cgctggagtc gtacctcatc
     2341 aagcccatcc agaggatcct caagtaccca cttctgctca gggagctgtt cgccctgacc
     2401 gatgcggaga gcgaggagca ctaccacctg gacgtggcca tcaagaccat gaacaaggtt
     2461 gccagtcaca tcaatgagat gcagaaaatc catgaagagt ttggggctgt gtttgaccag
     2521 ctgattgctg gtggtggatc aggtggagga tctatggtga gcaagggcga ggaggataac
     2581 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2641 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2701 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2761 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2821 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2881 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2941 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3001 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     3061 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     3121 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     3181 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     3241 ggcatggacg agctgtacaa gtaatga
//
