LOCUS       sequence-339298-        3186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses opto-Cdc42 
            (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Berlew EE, Yamada K, Kuznetsov IA, Rand EA, Ochs CC, Jaber Z, 
            Gardner KH, Chow BY
  TITLE     Designing Single-Component Optogenetic Membrane Recruitment Systems:
            The Rho-Family GTPase Signaling Toolbox.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):515-521. doi: 
            10.1021/acssynbio.1c00604. Epub 2022 Jan 3.
  PUBMED    34978789
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00604"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "515-521"
COMMENT     Sequence Label: opto-Cdc42
FEATURES             Location/Qualifiers
     source          1..3186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3118..3183
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg tggcggatca
      721 ggaggagggt caatgcagac aattaagtgt gttgttgtgg gcgatggtgc tgttggtaaa
      781 acatgtctcc tgatatccta cacaacaaac aaatttccat cggaatatgt accgactgtt
      841 tttgacaact atgcagtcac agttatgatt ggtggagaac catatactct tggacttttt
      901 gatactgcag ggcaagagga ttatgacaga ttacgaccgc tgagttatcc acaaacagat
      961 gtatttctag tctgtttttc agtggtctct ccatcttcat ttgaaaacgt gaaagaaaag
     1021 tgggtgcctg agataactca ccactgtcca aagactcctt tcttgcttgt tgggactcaa
     1081 attgatctca gagatgaccc ctctactatt gagaaacttg ccaagaacaa acagaagcct
     1141 atcactccag agactgctga aaagctggcc cgtgacctga aggctgtcaa gtatgtggag
     1201 tgttctgcac ttacacagaa aggcctaaag aatgtatttg acgaagcaat attggctgcc
     1261 ctggagcctc cagaaccgaa gaagagccgc aggggtggtg gatcaggtgg aggatctatg
     1321 gccacagacg caatcgacca ttactaccag aacagagacg gccccccttc caggagcttt
     1381 agcgacgaat acgactcata caataagaca gcccctctgc cacctctgag cgcctgccca
     1441 gagcgcgccc agacccctaa cagcaagaga atccccatca cacccaattc cgccaagagc
     1501 acctccgagt ctcctcggag aatcacattc tctcgcgacg caggcagctc caccaagggc
     1561 agcacccaga caagcccatc caaccagggc tccgtgtcta gccggaatgg ctctaacgga
     1621 aatggcctgg gcctgtcctc tgccggcaac ttcgccgatt tctttagctc cgaggtgttt
     1681 tatatcgtgc tgcacaatcc caccacagcc cacagattcc tgaagttttg ccagagcagg
     1741 gcctgttccg agaacctgga gtttctgcag aagatcgacg agtacaatag gctgatcgat
     1801 gaggccacac gcctgctgtc taccatctac agcacatacg tgagcagcga ggcccctaag
     1861 cagatcaaca tctctaatag ccacgccagg cgcctgagcc acgacatcaa gggagcaacc
     1921 cacaacgtgc tgcccggcct ggaggatatc ttcaatggct cccaggagca gatcgagaag
     1981 ctgctggcct ctgacctgta tcctagattt gtgaagcacc aggtgaccgc atccgccaca
     2041 atggccctgg caaacgacaa ggagaggtac ccaggcctgg gcgattgctt ctgtctgaca
     2101 gacccacgga gagccgataa tcccatcgtg ttcgcaagcg acggattcgt gagcgtgacc
     2161 ggctattccc gctctgatat catcccaagg aactgccgct tcctgcaggg cagctttacc
     2221 gaccggcagg ccacaaagag gctgcgcacc tccatcgaga atcgcgagga gacagtggag
     2281 ctgctgctga actatcggaa gaatggcgat cccttttgga acctgctgta cgtgtcccct
     2341 ctgctggacg gcaatggcga tgtgagattc tttctgggcg gccagatcaa ctgttctacc
     2401 acaatccaca gccggaccga cgtgctgaga atcctgagcc tgaacgacga ggatacaggc
     2461 tccttcatcg agggctctaa taagaccccc agcgtgcggt ccaaggagtc taacaatgcc
     2521 tccaactatg gcaagacatc cttctttaag tcttttaaga agtacaaggc caataccgtg
     2581 gagatcagag acgaggccgg catggagggc gagctgatcg ataggatcgg caagctgcag
     2641 ttcaagacac aggtggagga gttttacacc gcctattcta agtacctggt gctgagctat
     2701 cagccacagt cccagaacct gatcgtgaag tacttcagcc ccggcatcgt ggatatgctg
     2761 aacctgaata tgcctaatgg cctggtggcc ccaatcgtgg acaaggatat cttcaaggtg
     2821 ctgacagagc actccccttc ctctgtgcca cggaccttta agaacgtggt gagggaaggc
     2881 atcaaagcag gcagggccgt gtctgtggag acaggcctgc tgacaggcat cgaggagatc
     2941 aagaagtctc agaacatctt cagcggcacc acagccaatc gggagagagg ctggaagaag
     3001 gccgaggaga agtacgtgtg ccactttacc ccctgtaagg atgagatggg cagggtcggc
     3061 tgggtggtgc tgactgtcgc tcctaaactg gaaagatttg aattcggtgg tggatctgac
     3121 tacaaagatc atgacgggga ttataaggac cacgacatcg attacaagga tgacgatgac
     3181 aagtaa
//
