LOCUS       sequence-33932-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     Victoria Prince lab plasmids
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEM-T ZfHoxd11a
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(793..811)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(794..811)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(829..845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(829..845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggccgccatg gccgcgggat tgaggactcg agctcaagct tttttttttt tttttttttt
       61 agatataaaa ctgcaaagta cgtcctgtat ttttatttaa tacactgata catatacatg
      121 cttcaaatga ataaatgcat acacagcttt acctcaacga attcatttga tataaccaaa
      181 cctgtttgtt gcttattacg cagtgcgagt tgttacttga atgtattgaa tgtaagaact
      241 gaaattaaaa aaacataata aataaataca aacaaacaca aagcaactgc atttcaatgg
      301 caatcacaga gtatgaagag attgtcgggg tacctatgaa aactgataat gtccatctct
      361 gcggatacac tgcaataggt aatttgtgca gaagtgcagt tgcgaataaa atgactcttt
      421 tgtttttgtt tgatatagca agtattttgc atagtggtac aaatacaggg ctgaggtgaa
      481 aataagtcaa gtccgacgaa tggttgatgt cccacaagca tgacagaata gccttggtct
      541 ccagaagcgg ccaatatcat gactttcaga acaatgggtt tccagtgaaa tactggagtc
      601 gatctcggtt cagctttttc tctttcatcc tgcggttctg gaaccaaatc ttcacttgtc
      661 ggtctgtaag actgagcatt cttgacaact gcagacgttt ttccttattc atgtacacaa
      721 tcactagtgc ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca
      781 tagcttgagt attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt
      841 tcctgtgtga aattgttatc
//
