LOCUS       sequence-33933-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Victoria Prince lab plasmids
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEM-T ZfHoxd12a
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(787..805)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(788..805)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(823..839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(823..839)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(836..858)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    847..863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(871..877,878..895,896..901))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ccgccatggc cgcgggatta atgagtttat gacgaggcag aaacggaagg agctttcgga
       61 caggttggaa ctgagtgatc agcaagtcaa aatctggttt cagaatcgca ggatgaagaa
      121 aaagagactc atgatgcgcg agcatacctt cactatatac taagattttt tgttgtaatt
      181 ggatttattt ggggatcctt catacaagtg acttttcttt tacgctgata aaaatacatc
      241 tatttaactg gaaattaata tttaccacac accaattaaa agtaatttaa tattcaatcg
      301 gaatattttg agtttttatt tatttagtat cgctgcatgt gtacgtgtct ggtggtgtga
      361 atcgagaatc tgaatgatgt atatatccga ataaagtgag ctttatggtt aggaaccaca
      421 gcaaatttag gctcgtgtat gttggattaa aaacatacat tctggcccca ttaaaactac
      481 actgggcagc taatatagct aactggctca ttaaatattc agatgttcca taacatacct
      541 ttgtgttaaa aacctaattt gtatcttgca cactgaatac gctttgtttt gctttttctt
      601 aaccaaaagt gtaatgtaaa atatttatta aatgttgtaa attctgcctt ataataaact
      661 tttgcattat tgtgtgaaaa aaaaaaaaaa aaaagcttga gctcgagtcc tcaatcacta
      721 gtgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga tgcatagctt
      781 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt
      841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      901 agcct
//
