LOCUS       sequence-33935-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Prince VE, Moens CB, Kimmel CB, Ho RK
  TITLE     Zebrafish hox genes: expression in the hindbrain region of wild-type
            and mutants of the segmentation gene, valentino.
  JOURNAL   Development. 1998 Feb . 125(3):393-406.
  PUBMED    9425135
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 1998 Feb . 125(3):393-406."
COMMENT     Sequence Label: pBS ZfHoxd3a
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11..27
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(812..828)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..829)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(853..872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(854..872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(882..899)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(882..898)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(891..913)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgatt gaggactcga
       61 gctcaagctt tttttttttt ttttctctcc gagtgtagtt tttataaatt agtttagcta
      121 gtgaggtaat acaaaaagcg acccttcgct tgagcacaca gatgggatcc ttacaggtgc
      181 gtgagtctgg gcgcatcctg cagccttccc tgagacgcat gatgagagct gaggtctgtg
      241 tatgtggggt gggggtcaca cggtccgtgg tggtggttac ccggaatctg agcggcacag
      301 ctgtagtcca cactagcgga tgagggatgg ggaagatgac cgaggttgaa catggggccg
      361 gaggctggca ttgaatcaac gaagtttccc ccgacgtaca cggggctact ttgtaaatta
      421 gcattagcaa agctaccgtt cccctgcatg ctgtggtggt cgtactctgt tcccgggtac
      481 cttttctgct gcggtatgca gccgcccaat ggcgccgtgt atgccgccaa gccgtatata
      541 ttctgctgcg gcttggcgaa tgacggaggc gacggcgcgt cgtagctgag gctgtttacg
      601 ttcggaagct gaccagaata tccaatgtga ttcgagccac ttaacggcgg gctcctgtcc
      661 ggagagtgtc ctaagggaga gtgcattatg cctttagatt tctgatcttt tttgtacttc
      721 atccttctgt tttgaaacca gatctttatt tggcgctcgg tgaggttgag cagattggcc
      781 atctccactc ttctggggca atcaagctta tcgataccgt cgacctcgag ggggggcccg
      841 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
      901 cgtcgtgact ggg
//
