LOCUS       sequence-33940-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Prince VE, Joly L, Ekker M, Ho RK
  TITLE     Zebrafish hox genes: genomic organization and modified colinear 
            expression patterns in the trunk.
  JOURNAL   Development. 1998 Feb . 125(3):407-20.
  PUBMED    9425136
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 1998 Feb . 125(3):407-20."
COMMENT     Sequence Label: pGEM-T ZfHoxd9a
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(401..417)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(401..417)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(454..474)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(454..472)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(493..509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(493..509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(506..528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    517..533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(541..547,548..565,566..571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    586..607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtatcgata agcttgatta tgaagttgca cgaatcctga atttaacaga gcgccaggtg
       61 aaaatctggt ttcagaacag aagaatgaaa atgaagaaga tgaaccgaga gaggagcagc
      121 aaagatccgt aatggaaagc gtgcaagaag ctaatgcgcg tttctttagc acgcttggtt
      181 taggatacaa cttttgtccg tttttttgtt ttagtcactc aagattaaat tggcgtatat
      241 tctatttaat ttgatatatt tttaaatata ttttttctcc tcagataata attagtattt
      301 gagtaacttt aaataaaagg gtatttcaat cctcctaaat tgtaaaaaaa aaaaaaaaag
      361 cttgagctcg agtcctcaat cgaattcctg cagcccgggg gatccactag ttctagagcg
      421 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      481 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      541 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      601 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      661 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      721 ttcctcgctc actgactcg
//
