LOCUS       sequence-339403-       10239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses tBE-V5-mA3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Wang L, Xue W, Zhang H, Gao R, Qiu H, Wei J, Zhou L, Lei YN, Wu X, 
            Li X, Liu C, Wu J, Chen Q, Ma H, Huang X, Cai C, Zhang Y, Yang B, 
            Yin H, Yang L, Chen J
  TITLE     Eliminating base-editor-induced genome-wide and transcriptome-wide 
            off-target mutations.
  JOURNAL   Nat Cell Biol. 2021 May 10. pii: 10.1038/s41556-021-00671-4. doi: 
            10.1038/s41556-021-00671-4.
  PUBMED    33972728
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2021 May 10. pii: 10.1038/s41556-021-00671-4. doi: 
            10.1038/s41556-021-00671-4."
COMMENT     Sequence Label: pU6-ccdB-boxB-tBE-V5-mA3
FEATURES             Location/Qualifiers
     source          1..10239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        89..337
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     89..109
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     260..279
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        join(366..371,372..389,390..396)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             450..1109
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(516..535)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1451..1756
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1635..1654
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    1817..1835
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    1872..1890
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     misc_feature    2008..2125
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2172..2383
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2429..2438
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2894..2914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2915..3166
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             3800..3820
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4490..4510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4511..4567
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5081..5101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5102..5155
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5519..5539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5573..5590)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5579..5803
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5849..6277
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5936..5955)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6146..6167
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6286..6306)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6291..6620
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6471..6606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6533..6552
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6839..6858
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6927..7526
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6927..6946)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7423..7443
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7700..7821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7737..7756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7791..7810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7870..7886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7870..7886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7883..7905)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7894..7910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7918..7924,7925..7942,7943..7948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7963..7984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8101..8118)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8272..8860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8352..8371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9031..9822,9823..9891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9654..9673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9892..9996)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10071..10090)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtacgggcca gatatacgcg ttcgattagt gaacggatct cgacggtatc gatcacgaga
       61 ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt
      121 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
      181 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      241 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      301 cttggcttta tatatcttgt ggaaaggacg aaacaccggg agacgccgca ttaggcaccc
      361 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
      421 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      481 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      541 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      601 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      661 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      721 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      781 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      841 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
      901 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
      961 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1021 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1081 actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat
     1141 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     1201 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     1261 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     1321 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     1381 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     1441 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     1501 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     1561 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     1621 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     1681 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     1741 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgacgcgt
     1801 ctccgtttga gagctagggc cctgaagaag ggccctagca agttcaaata aggctagtcc
     1861 gttatcaact tgggccctga agaagggccc aagtggcacc gagtcggtgc tttttttaaa
     1921 gggcccgtcg actgcagagg cctgcatgca agcttggcgt aatcataaat tctcgacctc
     1981 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2041 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2101 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     2161 gccgccgtga ggggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     2221 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     2281 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     2341 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gacgcggttc
     2401 cggttaaggt gaacgcgtgc aggctggcgc caccatggcc tctaacttca cccagtttgt
     2461 gctggtggac aatggaggaa ccggcgatgt gacagtggca ccatccaact ttgccaatgg
     2521 catcgccgag tggatcagct ccaactctag gagccaggcc tacaaggtga cctgcagcgt
     2581 gcggcagtct agcgcccaga atagaaagta cacaatcaag gtggaggtgc caaagggagc
     2641 atggcgcagc tatctgaaca tggagctgac catccccatc ttcgccacaa attccgactg
     2701 tgagctgatc gtgaaggcca tgcagggcct gctgaaggat ggcaacccca tcccttctgc
     2761 catcgccgcc aatagcggca tctactctgg aggatctagc ggaggcagct ctggcagcga
     2821 gacaccagga acaagcgagt cagcaacacc agagagcagt ggcggcagca gcggcggcag
     2881 cagcggcgga tcccctaaga agaaacgcaa ggtgatgacc aacctgtctg acatcatcga
     2941 gaaggagaca ggcaagcagc tggtcatcca ggagagcatc ctgatgctgc ccgaagaagt
     3001 cgaagaagtg atcggaaaca agcctgagag cgatatcctg gtccataccg cctacgacga
     3061 gagtaccgac gaaaatgtga tgctgctgac atccgacgcc ccagagtata agccctgggc
     3121 tctggtcatc caggattcca acggagagaa caaaatcaaa atgctgtctg gaggcagcat
     3181 gggaccattt tgcctgggct gttctcaccg gaagtgctat agccccatca gaaacctgat
     3241 cagccaggag acattcaagt ttcacttcaa gaatctgggc tacgccaagg gccggaagga
     3301 taccttcctg tgctatgagg tgacaagaaa ggactgtgat agccccgtgt ccctgcacca
     3361 cggcgtgttt aagaacaagg acaatatcca cgccgagatc tgttttctgt actggttcca
     3421 cgataaggtg ctgaaggtgc tgtcccccag ggaggagttc aagatcacct ggtatatgtc
     3481 ttggagccct tgcttcgagt gtgccgagca gatcgtgcgc tttctggcca cacaccacaa
     3541 cctgagcctg gacatcttta gctcccggct gtacaacgtg caggatcctg agacacagca
     3601 gaatctgtgc agactggtgc aggagggagc acaggtggca gcaatggacc tgtatgagtt
     3661 caagaagtgt tggaagaagt ttgtggataa cggcggccgg agattccggc catggaagag
     3721 actgctgacc aacttccggt accaggacag caagctgcag gagatcctgc gcccttgcta
     3781 tatctccgtg ccatctagcg agaatctgta cttccagagc atgtcctcta gcaccctgtc
     3841 taatatctgc ctgaccaagg gcctgcccga gacaaggttc tgggtggagg gccggagaat
     3901 ggaccctctg agcgaggagg agttttactc ccagttctat aaccagaggg tgaagcacct
     3961 gtgctactat caccgcatga agccttacct gtgctatcag ctggagcagt tcaatggaca
     4021 ggcaccactg aagggatgcc tgctgtccga gaagggcaag cagcacgccg agatcctgtt
     4081 tctggataag atcagatcta tggagctgag ccaggtgacc atcacatgtt acctgacatg
     4141 gtccccatgc ccaaactgtg catggcagct ggcagccttc aagagggacc gcccagatct
     4201 gatcctgcac atctacacct ctaggctgta ttttcactgg aagcgcccct tccagaaggg
     4261 cctgtgctcc ctgtggcagt ctggcatcct ggtggacgtg atggatctgc cccagtttac
     4321 cgactgttgg acaaacttcg tgaatcctaa gcggccattt tggccctgga agggcctgga
     4381 gatcatcagc aggcgcacac agcggagact gaggcgcatc aaggagtcct ggggcctgca
     4441 ggacctggtg aacgattttg gcaatctgca gctgggaccc cctatgagcc ccaagaagaa
     4501 gaggaaagtc gctactaact tcagcctgct gaagcaggct ggggacgtgg aggagaaccc
     4561 tggacctatg ggcaacgcca gaacaagacg gcgggagcgc agagccgaga agcaggccca
     4621 atggaaagcc gctaattccg gcgggagcag tggcggatcc agtgggtccg aaacccctgg
     4681 cacaagcgaa agcgctaccc ctgagtccag cggagggtcc tcaggggaat tcatgaagag
     4741 catgtctagc atggtgtctg acacaagctg taccttcccc tcctctgatg gcatcttttg
     4801 gaagcactgg attcagacaa aggacggcca gtgtggctcc cctctggtgt ctaccaggga
     4861 tggcttcatc gtgggcatcc acagcgcctc caacttcaca aacaccaaca actactttac
     4921 atccgtgcct aagaacttca tggagctgct gaccaatcag gaggcacagc agtgggtgtc
     4981 tggatggcgc ctgaacgccg atagcgtgct gtggggcggc cacaaggtgt ttatggtgaa
     5041 gccagaggag cccttccagc ctgtgaagga ggccacccag cctaagaaga aacgcaaggt
     5101 ggagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaatggg
     5161 cgagtccctg tttaagggcc cacgggacta caaccccatc agctccacaa tctgccacct
     5221 gaccaatgag agcgatggcc acaccacatc cctgtatggc atcggcttcg gccccttcat
     5281 catcacaaac aagcacctgt ttcggagaaa caatggcacc ctgctggtgc agtctctgca
     5341 cggcgtgttc aaggtgaaga ataccacaac cctgcagcag cacctgatcg acggaaggga
     5401 tatgatcatc atcagaatgc ctaaggactt cccccctttt ccacagaagc tgaagtttcg
     5461 ggagccacag agggaggaga ggatctgcct ggtgacaacc aacttccaga cactcgagcc
     5521 caagaagaag cggaaggtgt aatctagagg gcccgtttaa accgctgatc agcctcgact
     5581 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5641 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5701 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5761 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5821 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5881 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5941 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6001 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6061 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6121 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6181 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6241 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6301 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6361 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6421 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6481 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6541 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6601 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6661 aagagacagg atgaggatcg tttcgcatgg aagcgggtag gcctttgggg cagcggccaa
     6721 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     6781 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     6841 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     6901 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     6961 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     7021 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     7081 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7141 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7201 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7261 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtgtcgc
     7321 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7381 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7441 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7501 gcatgacccg caagcccggt gcctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     7561 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7621 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7681 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7741 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7801 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7861 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7921 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7981 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8041 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8101 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8161 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8221 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8281 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8341 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8401 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8461 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8521 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8581 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8641 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8701 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8761 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8821 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8881 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8941 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9001 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9061 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9121 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9181 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9241 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9301 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9361 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9421 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9481 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9541 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9601 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9661 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9721 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9781 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9841 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9901 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9961 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10021 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
    10081 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    10141 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    10201 aatctgctta gggttaggcg ttttgcgctg cttcgcgat
//
