LOCUS       sequence-339419-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 plasmid targeting exon 3 of human FIP200..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Assar EA, Tumbarello DA
  TITLE     Loss of the Essential Autophagy Regulators FIP200 or Atg5 Leads to 
            Distinct Effects on Focal Adhesion Composition and Organization.
  JOURNAL   Front Cell Dev Biol. 2020 Aug 4;8:733. doi: 
            10.3389/fcell.2020.00733. eCollection 2020.
  PUBMED    32850845
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2020-08-4"; volume: "8:733. doi"; pages: " 
            10.3389/fcell.2020.00733. eCollection 2020"
COMMENT     Sequence Label: px459 sgFIP200
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        56..131
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        228..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tggctttata tatcttgtgg aaggacgaaa caccgtatgt atttctggtt aacacgtttt
       61 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      121 cgagtcggtg cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      181 ttttagcgcg tgcgccaatt ctgcagacaa atggctctag aggtacccgt tacataactt
      241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg g
//
