LOCUS       sequence-33944-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Prince VE, Joly L, Ekker M, Ho RK
  TITLE     Zebrafish hox genes: genomic organization and modified colinear 
            expression patterns in the trunk.
  JOURNAL   Development. 1998 Feb . 125(3):407-20.
  PUBMED    9425136
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 1998 Feb . 125(3):407-20."
COMMENT     Sequence Label: pGEM-T ZfHoxc5a
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(738..756)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(739..756)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(774..790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(774..790)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(787..809)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    798..814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(822..828,829..846,847..852))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    867..888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgccatggcc gcgggattgc agacgtatcg agattgccaa taacctctgc ttgaacgagc
       61 gccaaattaa aatatggttc cagaaccgtc gcatgaagtg gaagaaggac tcaaagttga
      121 aagtaaaagc aggactataa ataatgtgtt gcacgacttc aattacaaca gcctgaaaat
      181 aaaggcaatg ttaacccttg aactaaaaca acgaatgatt taaaattgat tttgttatta
      241 acaactgttg atgtctatca tttgtttaca atattatgtt gttatgtgaa tatgttaata
      301 ttttcaatat ttattgatag tgctctgtcc ccagtacggc agatgctaat ttgaataatg
      361 tatgagctta aacattgatt atgctttact aaactctctt gtatcatcct acataatata
      421 taccaaggcc tgaatacagt taagttgaca tatatgtacg tgcaaaaatg tggtgctttg
      481 cttgatcatt catcaatctg ttcattgcat ttttgatgct acttgtcttt atttttcatt
      541 gtactttctg cctaaataca tctataatac ctacacaaga atggtaaatc ccaattcatg
      601 atataaatgc aaaaactagt ctcatattaa aaaaaaaaaa aaaaagcttg agctcgagtc
      661 ctcaatcact agtgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg
      721 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
      781 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      841 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      901 tcactgcccg ctttccagtc gggaaacctg tcgt
//
