LOCUS       sequence-33945-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Papapetrou EP, Lee G, Malani N, Setty M, Riviere I, Tirunagari LM, 
            Kadota K, Roth SL, Giardina P, Viale A, Leslie C, Bushman FD, Studer
            L, Sadelain M
  TITLE     Genomic safe harbors permit high beta-globin transgene expression in
            thalassemia induced pluripotent stem cells.
  JOURNAL   Nat Biotechnol. 2011 Jan;29(1):73-8. doi: 10.1038/nbt.1717. Epub 
            2010 Dec 12.
  PUBMED    21151124
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1717"; 
            journalName: "Nat Biotechnol"; date: "2011-01"; volume: "29"; issue:
            "1"; pages: "73-8"
COMMENT     Sequence Label: pLM-fSV2A
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(442..501)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(571..600)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ggcgcccacc ccgaccccgc tcgccatgct attgccgccg ggggccagca gcccgccggg
       61 cagcgtgtac ttatccttct tcatgagcgt cttggttttc cgccggggcc ggtatttata
      121 atccgggtgc tccttcatgt gcagcgctcg cagccgctta gcctcgtcga tgaacggccg
      181 cttctccgtc tccgacaaaa gtttccactc ggcgcccagg cgcttgctga tctccgagtt
      241 gtgcatcttg gggttctcct gggccatctt gcgccgctgc ccgcgggacc acaccatgaa
      301 ggcattcatg ggccgcttga cgcggtccgg gctgtttttc tggttgccgc cggccgccgc
      361 cgcggtggag ttgccgccgc cgccccccga agtttgctgc gggcccggcg gcttcagctc
      421 cgtctccatc atgttgtaca tgggaccggg gttactttca acatcgccag cgagtttcaa
      481 caaagcgtag ttagtacatt gtccagatcc cgcacaagag ttccgtagct gttcaagttt
      541 gtgtttcaac tgttctcgtc gtttccgcaa caagtcctct tcagaaatga gcttttgctc
      601 ctctgcttgg acggacagga tgtatgctgt ggctttttta aggataacta ccttgggggc
      661 cttttcattg ttttccaact ccgggatctg gtcacgcagg gcaaaaaagc tccgttttag
      721 ctcgttcctc ctctggcgct ccaagacgtt gtgtgttcgc ctcttgacat tctcctcggt
      781 gtccgaggac ctggggctgg tgcattttcg gttgttgctg atctgtctca ggactctgac
      841 actgtccaac ttgaccctct
//
