LOCUS       sequence-339471-        5182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Source of Tol2 transposase driven by a mini-CAGS promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pKC-Tol2
FEATURES             Location/Qualifiers
     source          1..5182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     879..898
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             936..2885
                     /codon_start=1
                     /product="transposase of the Tol2 transposon from the 
                     medaka fish Oryzias latipes (Kawakami, 2007)"
                     /label=Tol2 transposase
                     /translation="MEEVCDSSAAASSTVQNQPQDQEHPWPYLREFFSLSGVNKDSFKM
                     KCVLCLPLNKEISAFKSSPSNLRKHIERMHPNYLKNYSKLTAQKRKIGTSTHASSSKQL
                     KVDSVFPVKHVSPVTVNKAILRYIIQGLHPFSTVDLPSFKELISTLQPGISVITRPTLR
                     SKIAEAALIMKQKVTAAMSEVEWIATTTDCWTARRKSFIGVTAHWINPGSLERHSAALA
                     CKRLMGSHTFEVLASAMNDIHSEYEIRDKVVCTTTDSGSNFMKAFRVFGVENNDIETEA
                     RRCESDDTDSEGCGEGSDGVEFQDASRVLDQDDGFEFQLPKHQKCACHLLNLVSSVDAQ
                     KALSNEHYKKLYRSVFGKCQALWNKSSRSALAAEAVESESRLQLLRPNQTRWNSTFMAV
                     DRILQICKEAGEGALRNICTSLEVPMFNPAEMLFLTEWANTMRPVAKVLDILQAETNTQ
                     LGWLLPSVHQLSLKLQRLHHSLRYCDPLVDALQQGIQTRFKHMFEDPEIIAAAILLPKF
                     RTSWTNDETIIKRGMDYIRVHLEPLDHKKELANSSSDDEDFFASLKPTTHEASKELDGY
                     LACVSDTRESLLTFPAICSLSIKTNTPLPASAACERLFSTAGLLFSPKRARLDTNNFEN
                     QLLLKLNLRFYNFE"
     polyA_signal    2930..3011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3135..3216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3326..3343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3497..4085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3577..3596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4228..5037)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4951..4970
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5029..5048
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      361 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      421 gggggggggc ggccaggggg gggggggggg gggggggggg gggggggcgc gcgccaggcg
      481 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      541 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      601 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      661 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      721 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      781 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagctagag cctctgctaa
      841 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
      901 gtctcatcat tttggcaaag aattttaagc tagccatgga ggaagtatgt gattcatcag
      961 cagctgcgag cagcacagtc caaaatcagc cacaggatca agagcacccg tggccgtatc
     1021 ttcgcgaatt cttttcttta agtggtgtaa ataaagattc attcaagatg aaatgtgtcc
     1081 tctgtctccc gcttaataaa gaaatatcgg ccttcaaaag ttcgccatca aacctaagga
     1141 agcatattga gagaatgcac ccaaattacc tcaaaaacta ctctaaattg acagcacaga
     1201 agagaaagat cgggacctcc acccatgctt ccagcagtaa gcaactgaaa gttgactcag
     1261 ttttcccagt caaacatgtg tctccagtca ctgtgaacaa agctatatta aggtacatca
     1321 ttcaaggact tcatcctttc agcactgttg atctgccatc atttaaagag ctgattagta
     1381 cactgcagcc tggcatttct gtcattacaa ggcctacttt acgctccaag atagctgaag
     1441 ctgctctgat catgaaacag aaagtgactg ctgccatgag tgaagttgaa tggattgcaa
     1501 ccacaacgga ttgttggact gcacgtagaa agtcattcat tggtgtaact gctcactgga
     1561 tcaaccctgg aagtcttgaa agacattccg ctgcacttgc ctgcaaaaga ttaatgggct
     1621 ctcatacttt tgaggtactg gccagtgcca tgaatgatat ccactcagag tatgaaatac
     1681 gtgacaaggt tgtttgcaca accacagaca gtggttccaa ctttatgaag gctttcagag
     1741 tttttggtgt ggaaaacaat gatatcgaga ctgaggcaag aaggtgtgaa agtgatgaca
     1801 ctgattctga aggctgtggt gagggaagtg atggtgtgga attccaagat gcctcacgag
     1861 tcctggacca agacgatggc ttcgaattcc agctaccaaa acatcaaaag tgtgcctgtc
     1921 acttacttaa cctagtctca agcgttgatg cccaaaaagc tctctcaaat gagcactaca
     1981 agaaactcta cagatctgtc tttggcaaat gccaagcttt atggaataaa agcagccgat
     2041 cggctctagc agctgaagct gttgaatcag aaagccggct tcagctttta aggccaaacc
     2101 aaacgcggtg gaattcaact tttatggctg ttgacagaat tcttcaaatt tgcaaagaag
     2161 caggagaagg cgcacttcgg aatatatgca cctctcttga ggttccaatg tttaatccag
     2221 cagaaatgct gttcttgaca gagtgggcca acacaatgcg tccagttgca aaagtactcg
     2281 acatcttgca agcggaaacg aatacacagc tggggtggct gctgcctagt gtccatcagt
     2341 taagcttgaa acttcagcga ctccaccatt ctctcaggta ctgtgaccca cttgtggatg
     2401 ccctacaaca aggaatccaa acacgattca agcatatgtt tgaagatcct gagatcatag
     2461 cagctgccat ccttctccct aaatttcgga cctcttggac aaatgatgaa accatcataa
     2521 aacgaggcat ggactacatc agagtgcatc tggagccttt ggaccacaag aaggaattgg
     2581 ccaacagttc atctgatgat gaagattttt tcgcttcttt gaaaccgaca acacatgaag
     2641 ccagcaaaga gttggatgga tatctggcct gtgtttcaga caccagggag tctctgctca
     2701 cgtttcctgc tatttgcagc ctctctatca agactaatac acctcttccc gcatcggctg
     2761 cctgtgagag gcttttcagc actgcaggat tgcttttcag ccccaaaaga gctaggcttg
     2821 acactaacaa ttttgagaat cagcttctac tgaagttaaa tctgaggttt tacaactttg
     2881 agtaggatcc agaattcatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2941 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3001 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     3061 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tcattgatga gtttggacaa
     3121 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3181 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3241 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3301 tgtggtcatt cagctgttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3361 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3421 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3481 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3541 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3601 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3661 ttctcccttc gggaaccgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3721 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3781 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3841 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3901 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3961 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4021 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4081 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4141 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc ctttttgcca
     4201 gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     4261 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     4321 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     4381 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     4441 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     4501 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     4561 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     4621 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     4681 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg
     4741 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     4801 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     4861 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg
     4921 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     4981 agcatccatg ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac
     5041 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg cagctgagat
     5101 cttagggatt actagtttaa acctagttat taatagtaat caattacggg gtcattagtt
     5161 catagcccat atatggagtt cc
//
