LOCUS       sequence-339475-       11355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral construct (3rd generation) for the expression of cell 
            cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS 
            G2) and miniAID-fused mCherry2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Capece M, Tessari A, Mills J, Vinciguerra GLR, Louke D, Lin C, 
            McElwain BK, Miles WO, Coppola V, Davies AE, Palmieri D, Croce CM
  TITLE     A novel auxin-inducible degron system for rapid, cell cycle-specific
            targeted proteolysis.
  JOURNAL   Cell Death Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4.
  PUBMED    37537305
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4."
COMMENT     Sequence Label: pLentiDP1.2
FEATURES             Location/Qualifiers
     source          1..11355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2259..3983
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             join(4011..4013,4014..4016,4017..4727)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4056..4077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4317..4336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4664..4685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4764..5093
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             5103..5159
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5160..5363
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             5364..6074
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5509..5527)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    6075..6663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6128..6148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6546..6557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6714..6734)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6719..7048
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6899..7034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7096..7143
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7162..7560
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7647..7880
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7952..8073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7989..8008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8043..8062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8113..8248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8268..8287)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8269..8287)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8297..8314)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8297..8313)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8306..8328)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8455..8910
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8542..8561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8752..8773
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8936..9040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9041..9109,9110..9901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9259..9278)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10072..10660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10561..10580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10814..10831
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10948..10969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10984..10989,10990..11007,11008..11014)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11022..11038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11027..11049
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11046..11062
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11046..11062
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11081..11101
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11083..11101
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11129..11355
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tggtttagtg aaccgtcaga tccgctagcg ctgccaccat gacatacttt cctgaagagg
     2281 tcgtcgaaca catttttagc ttcctgcctg cacagagaga tagaaacaca gtgagcctgg
     2341 tctgcaaagt gtggtacgag atcgaacgcc tgagccggag aggagtgttc gtcggcaact
     2401 gctatgctgt gagagcaggc agggtcgccg ctaggtttcc aaatgtgcgc gcactgaccg
     2461 tcaaggggaa accccacttc gccgacttta acctggtgcc ccctgattgg ggaggatacg
     2521 ccggcccttg gatcgaggca gccgctcgcg gctgtcatgg actggaggaa ctgcgcatga
     2581 agcgaatggt ggtctctgac gaaagtctgg agctgctggc tcggagcttc cctaggtttc
     2641 gcgcactggt gctgatttct tgcgaaggct tcagcaccga tggactggca gccgtggcct
     2701 cccactgtaa gctgctgcgg gagctggacc tccaggagaa tgaagtggag gatagaggcc
     2761 ccagatggct gtcttgcttc ccagactcat gtaccagcct ggtgtccctg aactttgcct
     2821 gcatcaaagg cgaagtgaat gctgggtccc tggagcggct ggtctcaaga agccccaacc
     2881 tgaggtctct gcggctgaac cggagcgtga gcgtggacac tctggctaag attctgctga
     2941 gaacccctaa cctggaggat ctgggaaccg gcaatctgac agacgatttc cagacagaat
     3001 cctactttaa actgacttct gccctggaga agtgtaaaat gctgaggagt ctgtcaggat
     3061 tctgggatgc ttcacccgtg tgcctgagct ttatctaccc tctgtgtgca cagctgacag
     3121 gcctgaacct gagctatgca ccaaccctgg acgccagtga tctgacaaag atgatctcac
     3181 gctgcgtgaa actccagcga ctgtgggtgc tggactgtat ttccgataag gggctccagg
     3241 tggtcgccag ctcctgcaag gacctccagg agctgagagt gttcccatct gatttttacg
     3301 tggccggata tagtgctgtc actgaggaag gcctggtggc agtctcactg ggatgcccaa
     3361 agctgaacag cctgctgtat ttctgtcatc agatgactaa tgctgcactg gtgaccgtcg
     3421 ccaagaactg ccctaatttc acccgatttc ggctgtgtat tctggaacca ggcaaacccg
     3481 acgtggtcac atcccagcca ctggatgaag ggtttggagc tatcgtgaga gagtgcaagg
     3541 gactccagag gctgagcatt tccggcctgc tgacagacaa agtgttcatg tacatcggca
     3601 agtatgctaa gcagctggag atgctgagca ttgcatttgc cggagactcc gataagggca
     3661 tgatgcacgt gatgaacggg tgtaagaatc tgcgaaaact ggaaatccgg gacagccctt
     3721 tcggggatgc cgctctgctg ggaaactttg ccagatacga gacaatgagg agcctgtgga
     3781 tgtctagttg caatgtgact ctgaagggct gtcaggtcct ggctagtaaa atgcctatgc
     3841 tgaacgtgga agtcattaat gagcgggacg ggtctaacga aatggaggaa aatcatggcg
     3901 acctgccaaa ggtggagaaa ctgtatgtgt atcggaccac cgcaggggca agagatgatg
     3961 ctcccaactt tgtgaagatt ctgggaggtt ctggtggttc tggtggttcc atggtgagca
     4021 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4081 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4141 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4201 ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     4261 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4321 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4381 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4441 acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     4501 tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4561 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4621 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4681 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagcct aggggatatc
     4741 catcacactg gcggccgctc gagatgaatc ccagtatgaa gcagaaacaa gaagaaatca
     4801 aagagaatat aaagaatagt tctgtcccaa gaagaactct gaagatgatt cagccttctg
     4861 catctggatc tcttgttgga agagaaaatg agctgtccgc aggcttgtcc aaaaggaaac
     4921 atcggaatga ccacttaaca tctacaactt ccagccctgg ggttattgtc ccagaatcta
     4981 gtgaaaataa aaatcttgga ggagtcaccc aggagtcatt tgatcttatg attaaagaaa
     5041 atccatcctc tcagtattgg aaggaagtgg cagaaaaacg gagaaaggcg ctgggcagcg
     5101 gcgccacaaa cttctctctg ctaaagcaag caggtgatgt tgaagaaaac cccgggccta
     5161 aggagaagag tgcttgtcct aaagatccag ccaaacctcc ggccaaggca caagttgtgg
     5221 gatggccacc ggtgagatca taccggaaga acgtgatggt ttcctgccaa aaatcaagcg
     5281 gtggcccgga ggcggcggcg ttcgtgaagg tatcaatgga cggagcaccg tacttgagga
     5341 aaatcgattt gaggatgtat aaaatggtga gcaagggcga ggaggataac atggccatca
     5401 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     5461 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     5521 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     5581 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     5641 ccgagggctt caattgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     5701 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     5761 acttcccctc cgacggcccc gtaatgcagt gtcgtaccat gggctgggag gcctccactg
     5821 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     5881 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     5941 agctgcccgg cgcctacaac gtcgacatca agttggacat cctttcccac aacgaggact
     6001 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     6061 agctgtacaa gtaaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6121 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6181 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6241 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6301 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6361 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6421 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6481 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6541 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6601 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6661 cgcctggaat tctgcagata tccggttagt aatgagtttg gaattaattc tgtggaatgt
     6721 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6781 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6841 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6901 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6961 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7021 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7081 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7141 gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt
     7201 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     7261 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     7321 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     7381 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     7441 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     7501 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     7561 gcacaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7621 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
     7681 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7861 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7921 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8101 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8161 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8221 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     8281 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8341 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8401 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8461 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8521 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8581 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8641 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8701 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8761 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8821 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8881 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8941 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9001 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9061 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9121 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9181 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9241 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9301 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9361 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9421 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9481 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9541 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9601 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9661 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9721 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9781 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9841 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9901 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9961 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10681 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10741 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    10801 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    10861 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10921 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10981 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    11041 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    11101 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
    11161 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
    11221 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
    11281 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
    11341 gctcgataca taaac
//
