LOCUS       sequence-339476-        3508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for recombinant His-Myc tag human PLD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake
            K, Nemazee D
  TITLE     Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory 
            triggering by multiple sensors.
  JOURNAL   Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.
  PUBMED    34620855
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-7"; volume: "12(1):5874. doi"; pages: " 
            10.1038/s41467-021-26150-w"
COMMENT     Sequence Label: SuExp His-Myc-hPLD3
FEATURES             Location/Qualifiers
     source          1..3508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          694..790
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             806..823
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             851..880
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             881..910
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             917..928
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2242..2376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2266..2285)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2320..2339
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2419..3007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2499..2518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3032..3406)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3407..3470)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgtcga gccgagtggt tgtgcctcca tagaagccac catgacccgc
      661 ctgaccgtgc tggccctgct ggccggcctg ctggtaagtt tagtcttttt gtcttttatt
      721 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
      781 ttacttctag gccagcagcc gcgcccatca tcatcatcat catggtccgc tagttgacgt
      841 cgccagcaat gaacaaaaac tcatctcaga agaggatctg gctagtatga ctggtggaca
      901 gcaaatgggt cgggatatcg aaggtcgtgg gctagctgaa tacggcgact tgcatctctt
      961 tgggcccaac cagcgcccag ccccctgcta tgacccttgc gaagcagtgc tggtggaaag
     1021 cattcctgag ggcctggact tccccaatgc ctccacgggg aacccttcca ccagccaggc
     1081 ctggctgggc ctgctcgccg gtgcgcacag cagcctggac atcgcctcct tctactggac
     1141 cctcaccaac aatgacaccc acacgcagga gccctctgcc cagcagggtg aggaggtcct
     1201 ccggcagctg cagaccctgg caccaaaggg cgtgaacgtc cgcatcgctg tgagcaagcc
     1261 cagcgggccc cagccacagg cggacctgca ggctctgctg cagagcggtg cccaggtccg
     1321 catggtggac atgcagaagc tgacccatgg cgtcctgcat accaagttct gggtggtgga
     1381 ccagacccac ttctacctgg gcagtgccaa catggactgg cgttcactga cccaggtcaa
     1441 ggagctgggc gtggtcatgt acaactgcag ctgcctggct cgagacctga ccaagatctt
     1501 tgaggcctac tggttcctgg gccaggcagg cagctccatc ccatcaactt ggccccggtt
     1561 ctatgacacc cgctacaacc aagagacacc aatggagatc tgcctcaatg gaacccctgc
     1621 tctggcctac ctggcgagtg cgcccccacc cctgtgtcca agtggccgca ctccagacct
     1681 gaaggctcta ctcaacgtgg tggacaatgc ccggagtttc atctacgtcg ctgtcatgaa
     1741 ctacctgccc actctggagt tctcccaccc tcacaggttc tggcctgcca ttgacgatgg
     1801 gctgcggcgg gccacctacg agcgtggcgt caaggtgcgc ctgctcatca gctgctgggg
     1861 acactcggag ccatccatgc gggccttcct gctctctctg gctgccctgc gtgacaacca
     1921 tacccactct gacatccagg tgaaactctt tgtggtcccc gcggatgagg cccaggctcg
     1981 aatcccatat gcccgtgtca accacaacaa gtacatggtg actgaacgcg ccacctacat
     2041 cggaacctcc aactggtctg gcaactactt cacggagacg gcgggcacct cgctgctggt
     2101 gacgcagaat gggaggggcg gcctgcggag ccagctggag gccattttcc tgagggactg
     2161 ggactcccct tacagccatg accttgacac ctcagctgac agcgtgggca acgcctgccg
     2221 cctgctctga atctagaagc tgatccagac atgataagat acattgatga gtttggacaa
     2281 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2341 ttatttgtaa ccattataag ctgcaataaa caagttggat atcctcgaga ctagtgggcc
     2401 cgtttaaaca catgtgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2461 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2521 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2581 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2641 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2701 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2761 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2821 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2881 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2941 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3001 atctcaagaa gatcctttga tcttttctac gtcagtcctg ctcctcggcc acgaagtgca
     3061 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
     3121 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
     3181 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
     3241 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
     3301 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
     3361 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatactc ttcctttttc
     3421 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata acgcgtcgcg
     3481 aggccatatg ggttaaccca tggccgtt
//
