LOCUS       sequence-339485-        3486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for recombinant His-Myc tag mouse PLD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake
            K, Nemazee D
  TITLE     Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory 
            triggering by multiple sensors.
  JOURNAL   Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.
  PUBMED    34620855
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-7"; volume: "12(1):5874. doi"; pages: " 
            10.1038/s41467-021-26150-w"
COMMENT     Sequence Label: SuExp His-Myc-mPLD3
FEATURES             Location/Qualifiers
     source          1..3486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          694..790
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             806..823
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             851..880
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             881..910
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             917..928
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2220..2354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2244..2263)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2298..2317
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2397..2985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2477..2496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3010..3384)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3385..3448)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgtcga gccgagtggt tgtgcctcca tagaagccac catgacccgc
      661 ctgaccgtgc tggccctgct ggccggcctg ctggtaagtt tagtcttttt gtcttttatt
      721 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
      781 ttacttctag gccagcagcc gcgcccatca tcatcatcat catggtccgc tagttgacgt
      841 cgccagcaat gaacaaaaac tcatctcaga agaggatctg gctagtatga ctggtggaca
      901 gcaaatgggt cgggatatcg aaggtcgtgg ggacttacat ctatttggac caaatcagcg
      961 cccagccccc tgctatgacc cctgcgaggc ggtgctggtg gagagcattc ctgagggtct
     1021 ggagtttccc aatgccacca cgagcaaccc ctccactagc caggcctggt tgggcctcct
     1081 tgccggtgct cacagcagcc tggacatcgc atccttctac tggaccctca caaataatga
     1141 cacccacacg caagagccct ctgcccagca gggtgaagag gttcttcagc agcttcaggc
     1201 actggcacct cgaggtgtaa aggttcgcat cgctgtgagc aaacccaatg ggcctctggc
     1261 tgatctgcag tctctgctac agagtggtgc ccaggtccgc atggtggaca tgcagaagct
     1321 gactcatggt gtcctgcaca ccaagttctg ggtggtggat cagacccact tctacctggg
     1381 cagtgccaac atggactggc ggtcgctgac ccaggtcaag gagctgggcg tggtcatgta
     1441 caactgcagc tgcctcgccc gggacctcac caagatcttt gaagcctatt ggttcctggg
     1501 ccaggcaggt agctccatcc catcaacctg gccgcggtcc tttgacaccc gatataatca
     1561 agaaacacca atggagatct gcctcaatgg caccccagct ctggcctacc tagcgagtgc
     1621 acccccacca ctgtgtccaa gtggccgcac cccagacctg aaggctctgc tcaacgtggt
     1681 ggacagtgcc cgaagcttca tctacattgc ggttatgaac tacctgccca ccatggagtt
     1741 ctctcatcca cgcaggttct ggccagcgat cgatgatggg ctgagacggg ctgcgtatga
     1801 acgaggcgtc aaagtgcgcc tgctcatcag ctgctgggga cactctgatc catcgatgcg
     1861 gtccttcctg ctctccctgg ctgcacttca tgacaaccat actcactccg acatccaggt
     1921 gaaactgttt gtggtcccta cggatgagtc ccaggcccga atcccctatg cccgtgtcaa
     1981 ccacaacaag tacatggtga ctgaacgtgc ctcatacatt ggaacctcca actggtctgg
     2041 aagctacttc acggagacag caggcacctc cctgctggtg acacagaacg ggcatggtgg
     2101 cttgcgcagt cagctggagg ctgttttcct gagagactgg gaatccccat acagccacga
     2161 tctcgacacc tcagccaaca gtgtgggcaa tgcctgccgc ctgctttaat ctagaagctg
     2221 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2281 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2341 gcaataaaca agttggatat cctcgagact agtgggcccg tttaaacaca tgtgtttttc
     2401 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2461 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2521 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2581 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2641 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2701 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2761 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2821 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2881 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2941 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3001 ttttctacgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg
     3061 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
     3121 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
     3181 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
     3241 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     3301 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     3361 aacggcactg gtcaacttgg ccatactctt cctttttcaa tattattgaa gcatttatca
     3421 gggttattgt ctcatgagcg gatacataac gcgtcgcgag gccatatggg ttaacccatg
     3481 gccgtt
//
