LOCUS       sequence-339489-        6784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FepA D12C, I14P . Please note that mutation numbers are not 
            based on start codon of insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6784)
  TITLE     Postle Lab Plasmids
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKP2185
FEATURES             Location/Qualifiers
     source          1..6784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..57
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(237..1115)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1142..1426
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1349..1368
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(3783..3800)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3953..4039
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4131..4158
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4177..4268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4269..4337,4338..5129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4487..4506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5171..5626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5258..5277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5468..5489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5737..6325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6226..6245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6479..6496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6662..6684
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
       61 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct
      121 gctcatgttt gacagcttat catcgatgca taatgtgcct gtcaaatgga cgaagcaggg
      181 attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat
      241 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
      301 ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca
      361 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
      421 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
      481 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
      541 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
      601 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
      661 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
      721 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
      781 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
      841 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
      901 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
      961 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
     1021 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
     1081 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
     1141 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
     1201 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
     1261 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
     1321 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
     1381 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg
     1441 gctagcagga ggaattcacc atgaacaaga agattcattc cctggccttg ttggtcaatc
     1501 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgact
     1561 gtactcccgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
     1621 ccgcagatga aatccgcaaa aacccggttg cccgcgatgt gtcgaagatc atccgtacca
     1681 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
     1741 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
     1801 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatactt
     1861 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
     1921 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggcg
     1981 agtggcacgg ctcctgggac gcatatttca atgcgccaga acataaagag gaaggtgcca
     2041 ccaaacgcac taactttagc ctgaccggtc cgctgggcga cgaattcagc ttccgtttgt
     2101 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
     2161 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggta atcaacaaag
     2221 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
     2281 gttacagccg ccagggtaac ctgtatgcgg gcgacaccca gaataccaac tccgattcct
     2341 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tacgcgctga
     2401 cctggaacgg tggctgggat aacggcgtga ccaccagcaa ctgggtgcag tacgaacaca
     2461 cccgtaactc gcgtattccg gaaggtctgg cgggcggtac cgaagggaaa tttaacgaaa
     2521 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
     2581 acctgccgat tgatttcctc gttaaccaga cgctgacgct gggtacggag tggaatcagc
     2641 aacggatgaa ggacttaagt tccaacaccc aggcactgac cggaacgaat accggtggcg
     2701 ctattgatgg cgtgagtacc accgaccgta gcccgtattc aaaagcagaa attttctcgc
     2761 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
     2821 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
     2881 taggcgatga cttcacgctg aaaatgggca tcgcccgtgc ttataaagcg ccgagcctgt
     2941 accagactaa cccgaactac attctctaca gtaaaggtca gggttgctat gccagcgcgg
     3001 gcggctgcta tctgcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
     3061 ttggtctgga gttcaaacgc gacgggtggc tggcgggcgt cacctggttc cgtaacgatt
     3121 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
     3181 tctatcagtg ggataacgtg ccgaaagcgg tggttgaagg tctggaagga tcgttaaacg
     3241 taccggttag cgaaacggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
     3301 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta tacgttgaac tcaacgctga
     3361 gctggcaggc acgggaagat ttgtcgatgc aaacgacctt cacctggtac ggcaagcagc
     3421 agccgaagaa gtacaactat aaaggtcagc cagcggttgg accggaaacc aaagaaatta
     3481 gtccttacag cattgttggc ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
     3541 ccggcggcgt ggacaatctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
     3601 cgggcgattt ggcaggggcc aactatatcg ccggtgccgg ggcgtatacc tataacgagc
     3661 cgggacgtac gtggtatatg agcgtaaaca cccacttctg atcatggtac ccggggatcc
     3721 tctagagtcg acctgcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc
     3781 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     3841 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     3901 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     3961 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     4021 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     4081 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     4141 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     4201 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4261 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4321 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4381 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4441 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4501 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4561 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4621 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4681 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4741 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4801 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4861 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4921 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4981 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5041 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5101 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5161 agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5221 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5281 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5341 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     5401 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5461 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     5521 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5581 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaaa ggatctaggt
     5641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5881 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6481 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     6541 gtgcggtatt tcacaccgca tagggtcatg gctgcgcccc gacacccgcc aacacccgct
     6601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagaag
     6721 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     6781 gcgc
//
