LOCUS       sequence-33951-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pDONR221 - myc ULK1 wt
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             90..119
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc ttcaccatgg aacaaaagct aatctccgag gaagacttgg
      121 gaggaataga gccgggccga ggcggcgtcg agaccgtggg caagttcgag ttctctcgca
      181 aggacctgat tggacacggc gccttcgcgg tggtcttcaa gggtcgacac cgcgagaagc
      241 acgacctgga ggtggccgtc aaatgcatta acaagaagaa ccttgccaag tcccaaacac
      301 tgctgggaaa ggaaatcaaa atcctgaagg aactaaagca cgaaaacatc gtggcgctgt
      361 atgacttcca ggaaatggct aattctgtct acctggtcat ggagtattgt aatggtggag
      421 acctggctga ctacctgcac actatgcgca cactgagtga agacactgtc aggcttttcc
      481 tacagcagat cgctggcgcc atgcggctgc tgcacagcaa gggcatcatc caccgggacc
      541 tgaagcccca aaacatcctg ctgtccaacc ctgggggccg ccgggccaac cccagcaaca
      601 tccgagtcaa gattgctgac tttggattcg ctcggtacct ccagagcaac atgatggcgg
      661 ccacactctg tggttctcct atgtacatgg ctcctgaggt cattatgtcc cagcactacg
      721 atggaaaggc tgacctgtgg agcattggca ccattgtcta ccagtgtctg acagggaagg
      781 ccccttttca ggccagcagc cctcaggatt tgcgcctgtt ttatgagaag aacaagacac
      841 tagttcctgc catcccccgg gagacatcag ctcccctgcg gcagctgct
//
