LOCUS       sequence-339512-       13660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoAMP4 circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Zhao EM, Lalwani MA, Chen JM, Orillac P, Toettcher JE, Avalos JL
  TITLE     Optogenetic Amplification Circuits for Light-Induced Metabolic 
            Control.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1143-1154. doi: 
            10.1021/acssynbio.0c00642. Epub 2021 Apr 9.
  PUBMED    33835777
REFERENCE   2  (bases 1 to 13660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00642"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1143-1154"
COMMENT     Sequence Label: EZ-L580
FEATURES             Location/Qualifiers
     source          1..13660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(64..95)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(266..513)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     479..497
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(1402..1422)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1452..1849)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(2512..2531)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2513..2531)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2538..2555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2538..2554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2547..2569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2695..3150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2782..2801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2992..3013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3506..3525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3625..3647
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3685..3703)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3787..3891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3892..3960,3961..4752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4110..4129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4923..5511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5412..5431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5665..5682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5799..5820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5835..5840,5841..5858,5859..5865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5873..5889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5878..5900
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5897..5913
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5897..5913
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5934..5952
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6278..6465
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     terminator      complement(7928..8093)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8014..8038
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8667..9971)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYDVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     CDS             complement(join(11025..13660,1..7))
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 gcttcatgct agccatgatt ttagcggccg cggtggcttt accaacagta ccggattgcc
       61 aagctggaag tcgagcttcc attatatacc ctctagtgtc tcttaagcat ggactaaagg
      121 ctacctataa cgcatggact aaaggctacc tataacgcat ggactaaagg ctacctataa
      181 cgcatggact aaaggctacc tataacgcat ggactaaagg ctacctactc gaagctggcg
      241 aactcacgta cccggcgata aggccgcaaa ttaaagcctt cgagcgtccc aaaaccttct
      301 caagcaaggt tttcagtata atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa
      361 aaatttgaaa tataaataac gttcttaata ctaacataac tataaaaaaa taaataggga
      421 cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg gggggagggc
      481 gtgaatgtaa gcgtgacata actaattaca tgatcattac tcgaggtcga cttaaatacc
      541 tgcttctacg gcaattctaa ctaaatctgc actcgttttt aagtttaatt tttccattac
      601 caaacctctg tgcattttga cagttttttc tgaaagaccc agtctggcag ccacttcttt
      661 atttcttagg cctgatgcaa ccagagttgt gacttctaat tgacgtggtg aaagagtttt
      721 taacatctcc gcagcacgtt ctctacgggc catgcccatg ttcggttgat cgtcatccac
      781 ttccacttgg ctaccaagga agtatagtaa ttcatcatcg tcatcataga ttggtgccac
      841 caaaactgca ttacgaaaag gagtaccatc ctttttgtaa ttcaaaatct caaccaacac
      901 gggtttatgc tcacgaacac cttgacggat cttatctgtt agccagggtt cagtaccaga
      961 tccctgtaag aacctacaat tacgcccaac acattcttcc tcagaatacc cagttagatc
     1021 ggtaaaggcc tggttgatag caatcaaagg attatcagcc aatctgggat cactaacaac
     1081 acttgctatg ggtgaggcct cgatcaaatc caatacccat tgggctggag gttggacttc
     1141 aactctagta tcatctgctc cgaattcacc tccatattcg tcgataccaa gtgcatcagt
     1201 aaacatctgt tcaaattcga aatctgccat gtccaacgcg ccatacggag cagaatcatg
     1261 tggtgtaaat ccggggccgg gagaatcccc atcacctaac atgtccagat caaaatcatc
     1321 aagagcatcg gcatgtgcca tagctacatc ctcaccgtct aaatgtaatt cgtcaccaag
     1381 tgaaacgtcc gttggtgggg ctacttttct cttttttttc gggctagcca tgattttagc
     1441 ggccgccaaa acttagatta gattgctatg ctttctttct aatgagcaag aagtaaaaaa
     1501 agttgtaata gaacaagaaa aatgaaactg aaacttgaga aattgaagac cgtttattaa
     1561 cttaaatatc aatgggaggt catcgaaaga gaaaaaaatc aaaaaaaaaa ttttcaagaa
     1621 aaagaaacgt gataaaaatt tttattgcct ttttcgacga agaaaaagaa acgaggcggt
     1681 ctcttttttc ttttccaaac ctttagtacg ggtaattaac gacaccctag aggaagaaag
     1741 aggggaaatt tagtatgctg tgcttgggtg ttttgaagtg gtacggcgat gcgcggagtc
     1801 cgagaaaatc tggaagagta aaaaaggagt agaaacattt tgaagctatc agctgtttcc
     1861 cgggagctgg aactcccacc tgcaacatgc gggatcctga gtaaataagc ggccgccacc
     1921 gcggtggagc tcagatcttg acaccgatta tttaaagctg cagcatacga tatatataca
     1981 tgtgtatata tgtataccta tgaatgtcag taagtatgta tacgaacagt atgatactga
     2041 agatgacaag gtaatgcatc attctatacg tgtcattctg aacgaggcgc gctttccttt
     2101 tttctttttg ctttttcttt ttttttctct tgaactccag aaaaaaaata taaaagagat
     2161 ggaggaacgg gaaaaagtta gttgtggtga taggtggcaa gtggtattcc gtaagaacaa
     2221 caagaaaagc atttcatatt atggctgaac tgagcgaaca agtgcaaaat ttaagcatca
     2281 acgacaacaa cgagaatggt tatgttcctc ctcacttaag aggaaaacca agaagtgcca
     2341 gaaataacag tagcaactac aataacaaca acggcggcta caacggtggc cgtggcggtg
     2401 gcagcttctt tagcaacaac cgtcgtggtg gttacggcaa cggtggtttc ttcggtggaa
     2461 acaacggtgg cagcggccgg ccatttaaat gtttaaacgg ccacgctggc cccctatagt
     2521 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     2581 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     2641 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     2701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3121 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     3181 gtgcggtatt tcacaccgca tagggtaata actgatataa ttaaattgaa gctctaattt
     3241 gtgagtttag tattaggatc tctacccttg gcgaaaagtc ctctgccaac aatgatgata
     3301 tctgatccac cacttacaac ttcgtcgagg gttctgtact gctgacccaa tatgcgtata
     3361 tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa
     3421 tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg
     3481 aaaaggtggt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3541 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3601 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3661 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3721 tgataataat ggtttcttag acgtcacgcg ccgaggacgt caggtggcac ttttcgggga
     3781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3841 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3901 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3961 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4021 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4081 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4141 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4201 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4261 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4321 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4381 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5041 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5701 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5761 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5821 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5881 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cggaattaac
     5941 cctcactaaa gggaacaaaa gctgggccgg ccatttaaat gtttaaacgg ccacgctggc
     6001 cgctgcacgg tcctgttccc tagcatgtac gtgagcgtat ttccttttaa accacgacgc
     6061 tttgtcttca ttcaacgttt cccattgttt ttttctacta ttgctttgct gtgggaaaaa
     6121 cttatcgaaa gatgacgact ttttcttaat tctcgtttta agagcttggt gagcgctagg
     6181 agtcactgcc aggtatcgtt tgaacacggc attagtcagg gaagtcataa cacagtcctt
     6241 tcccgcaatt ttctttttct attactcttg gcctcctcta gtacactcta tattttttta
     6301 tgcctcggta atgattttca tttttttttt tccacctagc ggatgactct ttttttttct
     6361 tagcgattgg cattatcaca taatgaatta tacattatat aaagtaatgt gatttcttcg
     6421 aagaatatac taaaaaatga gcaggcaaga taaacgaagg caaagaggct ctatatgtat
     6481 ataaagctgc ggatgccttc tgatttaatc tagttctagt ttacttcagg tttacttaaa
     6541 gttgagataa ttgaagcaaa gagaagcatc gagcaaagga tggcgtttgt taagagggtt
     6601 acgcaggaga cgaatataca gctggcgctg gatcttgacg gtgggtctgt ttctgtacgg
     6661 gagagcatac tgggcaagga atatgctagt ggtgatgggc agaccatcca tgtgcacact
     6721 ggagttgggt ttttggacca catgttgact gcgctggcga agcatggcgg gtggtctctg
     6781 atcctggagt gtatagggga cttgcacatc gatgaccacc acactgttga ggactgtggg
     6841 attgcgctgg gccaagcgtt caaggaggcg cttggctccg tgcgtggtat caagaggttc
     6901 gggcatgggt ttgcaccact ggacgaggcg ctgagccgcg ctgtggttga cttctccaat
     6961 aggcctttcg ccgtggtgga gctgggcctg aagcgagaac gcataggcca gctatccaca
     7021 gagatgatcc cgcacttctt ggagagtttc gccactgagg cgcgtatcac catgcatgtg
     7081 gactgtctgc ggggcaccaa cgaccaccac cgctccgaat cagctttcaa ggcgctcgcc
     7141 atcgccatca gagaggcaag aacacctacg ggtcgcgatg acgttccatc cactaagggt
     7201 gtcctagcat agaacacagc ccacagctac caccatcaac aatatttata tagataacgt
     7261 acacatagaa atcacacaaa cagagtattt attcttaact acatgaacta ccatcagacc
     7321 gtctgggcca ctatataatg tgctattcat aaacgtgatc actctacgta gcaggcgacc
     7381 ccaggtgaaa atttttcagc gagctgccag attgtcaggt gaaaaactga aaaaaacttc
     7441 tgggcgatga gcttgtggcg ggaaattaag tatataaagc agttagtttc ttctgcttct
     7501 tgtggttctg ggtattcttg taatatttgg aataatagaa aaccaagact actgcctttc
     7561 ttttcatatt atagaggaag aaatacgcac gaacacgata tagaggtaaa ttttttgcaa
     7621 aaagatacca aactataaaa gaaatagaag actaaactac aaaaaataaa gaaagtaata
     7681 catatatcca atatgactga gttagcagag caagtttcca tgcctgaagg gcaacaaaga
     7741 cgtggtggtt acgttccacc tcacatccgt aacggtggtg gcagaagagg cgattctgct
     7801 cctcgtggtg gtttcaacaa cggtggcggc cgtggtggtt tcttcagaaa caacggtgac
     7861 cgtggtggat tcggctttgg gtacctccgg atatgttggc gcgccttaat ttattcgccg
     7921 gcgataagag cgacctcatg ctatacctga gaaagcaacc tgacctacag gaaagagtta
     7981 ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt
     8041 atttttttta taacttattt aataataaaa atcataaatc ataagaaatt cgctgatcat
     8101 tattattgga agtacaagtt ttcagaacca gccttcttgt acaaagaagt aatagattct
     8161 tgtgcacaag acataggaag ttctcgaacc gcttcatcga tattcacagc tgtttttttc
     8221 accaggtctt ctccctcttt cactgcggtt tcttcaatga cattgcgaac tggttctacg
     8281 tgcttgctcc cgtctagttg aactccaata aagtattgta cttctccctt ctgatcacgc
     8341 ataggttgca agtggaaaat gttccagaac ttctttccgc tcttggtgta gttgatgagc
     8401 tgaactgtca cttcggtttg gttatcaata gcatttcgaa tcttcttcac tgtggttaga
     8461 tcagtctctg gaccttgtag aaacctgcaa tttcttccaa gaatttcttc acggctatat
     8521 tccgtgagct ccaagaaact atcagatgca aaaataatgg gattatcggg aagcctagga
     8581 tcagtgataa taaaattctt ctcgatacgt tcgagtgtag tagctagatc aatacccttt
     8641 ctcatctgtc ctccagcact ctcgagtaaa ctataatgcg agatattgct aacgtttaat
     8701 gtggagccca tcatgttact tttataaacg ctctcgatta acctgtgtaa tatcagagca
     8761 tccatcaagg tgggaaagcc ttcgaaatcg aaaccttgca ttacaaattg tgagggtaat
     8821 tcaggaattt tctttgtatt gaagtggaag tcagagatag attgatacag tcgatgaata
     8881 ttaccgacaa tggcattata atttcgtaaa tgatatactt ccatgatttc tttacctgca
     8941 tcataccccg ggtctaaagg agcttgttgt ccattagcta gcgggaagtc gtttgctcta
     9001 gttccactgt agtaaaggac cagatttgaa atttctgcga atccggcatc gccttcaagt
     9061 ttcaaatctc ccttggtacc gtgaatatca atgaccaaat ttttggtaaa ttttttggta
     9121 ggtttgccac ctttgaaact gcatgacact ggaacattgc catttaacaa tgtgccttgg
     9181 aataaaagat gatccggtac tgtctttggg actcgctggc ccaatcggtt accacgctca
     9241 tctatcagct cttgctctgg aatattattg aaaaccattg catttatcct ggaaaagtac
     9301 gaacttgtca tgtattgtaa aatatcgatt gtgtgaccaa atgttgtggt taccagatct
     9361 acaccgttcc cgatttcata gatgtatttt ggtgatttaa caggcctttc gtagccgtac
     9421 caaccgccat ttccagcaat ctcgatagaa ttaatgtcgc cgatatagcc ttgagatatt
     9481 aattcttttg ctctcaaaat atatggtgat ttacgacctt gtaaagagat gatggtttga
     9541 accccacgtt cagcagcagc cttataaatg gattcggctt gatctagtga acatgcaagg
     9601 gcccattcta cgaaaagata cttgaggttc ggattatttt tggagaattc caagagaggc
     9661 ataacaacgt cataatgact ggccacttgg atagctatca ctatcatatc tatagtggaa
     9721 gatgatgcaa atgactctaa agtgggaaaa gcagtggcat tactcaattt tagacgctgg
     9781 atggtggcaa tagaagtctc aatttttgga ctgtataagg cagtgatttg aaattgtgac
     9841 gatagttgca gtatggcggg gtaatgtgtc ttgattgccc atcctttggc tgcgttgaga
     9901 ccgacgaatc cgactcttat gggagctgca ttaggcacgg ttgagaccga agatctcttg
     9961 ttgtagtcca tgctagccat gattttagcg gccgccaggt aattggacaa ataaatacgt
    10021 gtattaagtt tggattcttt ggtagatagc caatcgaggg aacaattaaa aaaaagaaag
    10081 aaataaaaga caagaagaag agcgaaaaag gggaaagtga tgaggaaaag aacaatagat
    10141 agatccagaa gatgatggta acgcgcaaac actaccgcta tatataccta caaaactctc
    10201 attctctcat ttcctgcttc gttcatttcg agtttccaag ggtatggtac ggtgctatct
    10261 gcccgtgctt cccctccttt gctttgctaa gaaaaatttt cttttgtcag cagcaacaac
    10321 acgctacgct ctgcgctgtg ctacgaagtg gtctctgtgg caacggggac attgtaccca
    10381 tgcccgctcg cagggaggtc tgggtgtggg gcgtgtgcga cctcgttgcc atggcgatcg
    10441 cgcgtgggtg tggtctttcc ccaaacggtt gcccgttgct ccggatttcg catagttgtc
    10501 gcgtaaacaa aaaacacgag agagggcgga cggcgctgaa tagtggaaga tcgcgcgatg
    10561 tcaagtaaat aagcttgtat ttgtatttgt atgtgtttat gtgtttgtgt ggttgagaat
    10621 tttattttcc tcctagtttt ttcttttttg agtgcggagg ggcagtgctc taaactttgc
    10681 ctgcttcttg ttcgaccccg ggtataccgg cgataaatat gatacacggt ccaatggata
    10741 aacatttttt atcaacacta tgatatataa atataatagt ttttcgtata tatattcctt
    10801 tttttggtca atttttgaaa ttttcgtaga aaagggagag acaaaacaca ttatatcaat
    10861 gaaaacgtac aaaaagtaga taaagtcagt gcttaaacac gtcttttcct taaaaatact
    10921 ttattatttt tattttattg agagggtggt ttaaaaatag aaatagagag agaggtacat
    10981 acataaacat acgcgcacaa aagcagagat gatcattact cgagctcttt ttttgggttt
    11041 ggtggggtat cttcatcatc gaatagatag ttatatacat catccattgt agtggtatta
    11101 aacatccctg tagtgattcc aaacgcgtta tacgcagttt ggtccgtcca accaggtgac
    11161 agtggttttg aattattacc atcatcaatt ttactagccg tgatttcatt attcatgaag
    11221 ttatcatgaa cgttagagga ggcaattggt tgtgaaagcg cttgagaatt tgtttgagtt
    11281 gttatgaggt tcggaccgtt gctactgtta gtgaaagtga aggacaatga gctatcagca
    11341 atattcccac tttgattaaa attggcgcca ccaaacaaag cagacggggt cagtggcact
    11401 aatgattgca gctgttgctg ttgccctaga aaaggcgtga ctgagcgatg cgaaggtgtg
    11461 cttcttggta ttgtcactgg agagttacga gagggtggac ggttagataa cagcttgact
    11521 agatcactga aacttgctcc tgatttcaat ggcacaggtg aaggccctac tgagccagga
    11581 gaaacatatt taacactgat attgttgaca ttttcctccg gaagagtagg gtattgggcg
    11641 atagttgcag aaccgacaat atttttaatg gcgctaccat tactattgtt ataactgata
    11701 tgcggtaatg ggattgcaca ctgtgataac agaaacggcg cacatacctc ttccagtact
    11761 tgaatgtatt tttcacaagt ctggatttta aaagtggcca gtttttttaa tagcatcaga
    11821 acagtgttaa tttgttgtaa taattgtgcg gtctcgttat tctcagcatt cgattttgag
    11881 tttgagagta gagtctttat gggtactagg actgcattga acaagtaata agaacaattc
    11941 caggcaaaat atggggtgac attatgattg tccatatagc tacttacaga cataacagtt
    12001 ctttgtgctg catcgcttaa catgatggag catcgtttaa cttcataact ttgatgatca
    12061 ttttgatcct gttctagttg tgactttttc tgggtaaaat tagtgaaaaa atctcttaat
    12121 acataaatga taagagacaa ctgtttccac ttcagttcga atcttgtaaa ggatagccaa
    12181 gggtgttcct tcaacaaatt ggttagagcg gtggtggaaa tatccatttg taaaaacttt
    12241 ggtgcctgtc tcgaaacctc ctcaatctca ttacaaatca tcaagcattt ttttgcacat
    12301 ataggacttt tttctgcagt tactgttttg tctagttcat agatttttgt gaaaacttgt
    12361 aagagccttg ctgtttcaat gatgccatga tatatggtgg gacctgttgt ggtacgctgc
    12421 acatcgtcga cagaagaagg gaaggagatt gtattctgag aaagctggat ggatcgacca
    12481 taaagcaggg acaattggat ctcccaagag tagacagacc accaaattcg gcgtctttgt
    12541 tccagaatgc tgctatcact gaaggacgag gggaggtccc tattcaagcc caatgatatg
    12601 gccattctta tggaaaagct gtgaaaatta tagctagtat ttgttttctg cctccactgt
    12661 gtatatcgcg acagaagatg tagggctgtc accaaaatta tggaacctga ctcgaagacc
    12721 ttgctcgtca aatgagattt agcattttga tagtaaaaaa catctatatc agtagattcc
    12781 ccctctatac accaggctcc aatggctaat atgcagttaa aaaggatttg ccattgatcc
    12841 ttcgacgcga tttcaatctg gttattatac aacatcatta gcgtcggtga gtgcacgata
    12901 gggcagtagg ggtgaaaatt attgagataa ctttgaagta aacgggatgt tgtggatcta
    12961 gaagccaacg tgtatctatc cgtaatcatg gtcgggagcc tgttaacgtt agagttcgtg
    13021 taattttccg gtttaaagcc aatagatcga agaatacata agagagaacc gtcgccaaag
    13081 aacccattat tgttggggtc cgttttcagg aagggcaagc catccgacat gtcatcctct
    13141 tcagaccaat caaatccatg aagagcatcc ctgggcataa aatccaacgg aattgtggag
    13201 ttatcatgat gagctgccga gtcaatcgat acagtcaact gtctttgacc tttgttacta
    13261 ctctcttccg atgatgatgt cgcacttatt ctatgctgtc tcaatgttag aggcatatca
    13321 gtctccactg aagccaatct atctgtgacg gcatctttat tcacattatc ttgtacaaat
    13381 aatcctgtta acaatgcttt tatatcctgt aaagaatcca ttttcaaaat catgtcaagg
    13441 tcttctcgag gaaaaatcag tagaaatagc tgttccagtc tttctagcct tgattccact
    13501 tctgtcagat gtgccctagt cagcggagac cttttggttt tgggagagta gcgacactcc
    13561 cagttgttct tcagacactt ggcgcacttc ggtttttctt tggagcactt gagcttttta
    13621 agtcggcaaa tatcgcatgc ttgttcgata gaagacagta
//
