LOCUS       sequence-339514-        8768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coselection for HDR in human cells. Vector for dual expression of 
            ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two 
            independent U6 promoters. Cloning of oligos using BbsI sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Levesque S, Mayorga D, Fiset JP, Goupil C, Duringer A, Loiselle A, 
            Bouchard E, Agudelo D, Doyon Y
  TITLE     Marker-free co-selection for successive rounds of prime editing in 
            human cells.
  JOURNAL   Nat Commun. 2022 Oct 7;13(1):5909. doi: 10.1038/s41467-022-33669-z.
  PUBMED    36207338
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-7"; volume: "13(1):5909. doi"; pages: " 
            10.1038/s41467-022-33669-z"
COMMENT     Sequence Label: pX330_ATP1A1_G7_Dual_sgRNA
FEATURES             Location/Qualifiers
     source          1..8768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     misc_RNA        complement(501..576)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(595..843)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     rep_origin      complement(905..1493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(985..1004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1664..2455,2456..2524))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2287..2306
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2525..2629)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2697..2715
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2753..2775)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2875..2894
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2911..3366)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3048..3069)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3260..3279
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(3533..3608)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3629..3877)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    3888..4095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4084..4101
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4129..4176)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(4177..8277)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4177..8277)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8302..8322)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8329..8394)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      8394..8403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8415..8642
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
ORIGIN
        1 cccccccccc cgcccccatc gctgcacaaa ataattaaaa aataaataaa tacaaaattg
       61 ggggtgggga ggggggggag atggggagag tgaagcagaa cgtggggctc acctcgacca
      121 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
      181 tgtactgggc acaatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
      241 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
      301 attgacgtca atggaaagtc cctattggcg ttactattga cgtcaatggg cgggggtcgt
      361 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgggtacc tctagagcca
      421 tttgtctgca gaattggcgc acgcgctaaa aacggactag ccttatttta acttgctatt
      481 tctagctcta aaacaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
      541 agccttattt taacttgcta tttctagctc taaaacaggt cttctcgaag acccggtgtt
      601 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      661 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      721 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattccaat
      781 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      841 ctcacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1321 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1441 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1501 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1561 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1621 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1681 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1741 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1801 cgcggcttcc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1861 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1921 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1981 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2041 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2101 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2161 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2221 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2281 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2341 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2401 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2461 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2521 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2581 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2641 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2701 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     2761 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     2821 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     2881 cagagcagat tgtactgaga gtgcaccata aaattgtaaa cgttaatatt ttgttaaaat
     2941 tcgcgttaaa tttttgttaa atcagctcat tttttaacca atagaccgaa atcggcaaaa
     3001 tcccttataa atcaaaagaa tagcccgaga tagagttgag tgttgttcca gtttggaaca
     3061 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3121 gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta
     3181 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3241 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctaag gcgctggcaa
     3301 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3361 gcgcgtacta tggttgcttt gacgtatgcg gtgtgaaata ccgcacagat gcgtaaggag
     3421 aaaataccgc atcaggcgcc cctgcagggc catttgtctg cagaattggc gcacgcgcta
     3481 aaaacggact agccttattt taacttgcta tttctagctc taaaacaaaa aagcaccgac
     3541 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc
     3601 tctaaaacca ctactatcga tctgtgacgg tgtttcgtcc tttccacaag atatataaag
     3661 ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata
     3721 attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta
     3781 atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc ttgtatcgta
     3841 tatgcaaata tgaaggaatc atgggaaata ggccctcacg cggccgctcc ccagcatgcc
     3901 tgctattctc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
     3961 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
     4021 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
     4081 aactagaagg cacagtcgag gctgatcagc gagctctagg aattcttact ttttcttttt
     4141 tgcctggccg gcctttttcg tggccgccgg ccttttgtcg cctcccagct gagacaggtc
     4201 gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt ccagcacctc
     4261 tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga aggcggcagg
     4321 ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct ctctgatggg
     4381 cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt cggccaggat
     4441 cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt gcttgtgctg
     4501 ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct tctcatagtg
     4561 gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt ttcccttctg
     4621 cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga acagggagta
     4681 cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg cttccagaaa
     4741 gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca gcagctcttt
     4801 cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca gcacagaata
     4861 ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct tctttctggc
     4921 gatcagctta tcgctgttcc tcttgggcag gatagactct ttgctgaagc cgcctgtctg
     4981 cacctcggtc tttttcacga tattcacttg gggcatgctc agcactttcc gcacggtggc
     5041 aaaatcccgg cccttatccc acacgatctc cccggtttcg ccgtttgtct cgatcagagg
     5101 ccgcttccgg atctcgccgt tggccagggt aatctcggtc ttgaaaaagt tcatgatgtt
     5161 gctgtagaag aagtacttgg cggtagcctt gccgatttcc tgctcgctct tggcgatcat
     5221 cttccgcacg tcgtacacct tgtagtcgcc gtacacgaac tcgctttcca gcttagggta
     5281 ctttttgatc agggcggttc ccacgacggc gttcaggtag gcgtcgtggg cgtggtggta
     5341 gttgttgatc tcgcgcactt tgtaaaactg gaaatccttc cggaaatcgg acaccagctt
     5401 ggacttcagg gtgatcactt tcacttcccg gatcagcttg tcattctcgt cgtacttagt
     5461 gttcatccgg gagtccagga tctgtgccac gtgctttgtg atctgccggg tttccaccag
     5521 ctgtctcttg atgaagccgg ccttatccag ttcgctcagg ccgcctctct cggccttggt
     5581 cagattgtcg aactttctct gggtaatcag cttggcgttc agcagctgcc gccagtagtt
     5641 cttcatcttc ttcacgacct cttcggaggg cacgttgtcg ctcttgcccc ggttcttgtc
     5701 gcttctggtc agcaccttgt tgtcgatgga gtcgtccttc agaaagctct gaggcacgat
     5761 atggtccaca tcgtagtcgg acagccggtt gatgtccagt tcctggtcca cgtacatatc
     5821 ccgcccattc tgcaggtagt acaggtacag cttctcgttc tgcagctggg tgttttccac
     5881 ggggtgttct ttcaggatct ggctgcccag ctctttgatg ccctcttcga tccgcttcat
     5941 tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg ttctctctgg ccatttcgat
     6001 cacgatgttc tcgggcttgt gccggcccat cactttcacg agctcgtcca ccaccttcac
     6061 tgtctgcagg atgcccttct taatggcggg gctgccggcc agattggcaa tgtgctcgtg
     6121 caggctatcg ccctggccgg acacctgggc tttctggatg tcctctttaa aggtcaggct
     6181 gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag ccgtcggact tcaggaaatc
     6241 caggattgtc ttgccggact gcttgtcccg gatgccgttg atcagcttcc ggctcagcct
     6301 gccccagccg gtgtatctcc gccgcttcag ctgcttcatc actttgtcgt cgaacaggtg
     6361 ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc tcaaacagtg tcagggtcag
     6421 cacgatatct tccagaatgt cctcgttttc ctcattgtcc aggaagtcct tgtccttgat
     6481 aattttcagc agatcgtggt atgtgcccag ggaggcgttg aaccgatctt ccacgccgga
     6541 gatttccacg gagtcgaagc actcgatttt cttgaagtag tcctctttca gctgcttcac
     6601 ggtcactttc cggttggtct tgaacagcag gtccacgatg gcctttttct gctcgccgct
     6661 caggaaggcg ggctttctca ttccctcggt cacgtatttc actttggtca gctcgttata
     6721 cacggtgaag tactcgtaca gcaggctgtg cttgggcagc accttctcgt tgggcaggtt
     6781 cttatcgaag ttggtcatcc gctcgatgaa gctctgggcg gaagcgccct tgtccaccac
     6841 ttcctcgaag ttccaggggg tgatggtttc ctcgctcttt ctggtcatcc aggcgaatct
     6901 gctgtttccc ctggccagag ggcccacgta gtaggggatg cggaaggtca ggatcttctc
     6961 gatcttttcc cggttgtcct tcaggaatgg gtaaaaatct tcctgccgcc gcagaatggc
     7021 gtgcagctct cccaggtgga tctggtgggg gatgctgccg ttgtcgaagg tccgctgctt
     7081 ccgcagcagg tcctctctgt tcagcttcac gagcagttcc tcggtgccgt ccatcttttc
     7141 caggatgggc ttgatgaact tgtagaactc ttcctggctg gctccgccgt caatgtagcc
     7201 ggcgtagccg ttcttgctct ggtcgaagaa aatctctttg tacttctcag gcagctgctg
     7261 ccgcacgaga gctttcagca gggtcaggtc ctggtggtgc tcgtcgtatc tcttgatcat
     7321 agaggcgctc aggggggcct tggtgatctc ggtgttcact ctcaggatgt cgctcagcag
     7381 gatggcgtcg gacaggttct tggcggccag aaacaggtcg gcgtactggt cgccgatctg
     7441 ggccagcagg ttgtccaggt cgtcgtcgta ggtgtccttg ctcagctgca gtttggcatc
     7501 ctcggccagg tcgaagttgc tcttgaagtt gggggtcagg cccaggctca gggcaatcag
     7561 gtttccgaac aggccattct tcttctcgcc gggcagctgg gcgatcagat tttccagccg
     7621 tctgctcttg ctcagtctgg cagacaggat ggccttggcg tccacgccgc tggcgttgat
     7681 ggggttttcc tcgaacagct ggttgtaggt ctgcaccagc tggatgaaca gcttgtccac
     7741 gtcgctgttg tcggggttca ggtcgccctc gatcaggaag tggccccgga acttgatcat
     7801 gtgggccagg gccagataga tcagccgcag gtcggccttg tcggtgctgt ccaccagttt
     7861 ctttctcagg tggtagatgg tggggtactt ctcgtggtag gccacctcgt ccacgatgtt
     7921 gccgaagatg gggtgccgct cgtgcttctt atcctcttcc accaggaagg actcttccag
     7981 tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct cttgcagata
     8041 gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca gccgggtggc
     8101 ctcggctgtt tcgccgctgt cgaacagcag ggctccgatc aggttcttct tgatgctgtg
     8161 ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact cgtcggtgat
     8221 cacggcccag cccacagagt tggtgccgat gtccaggccg atgctgtact tcttgtcggc
     8281 tgctgggact ccgtggatac cgaccttccg cttcttcttt ggggccatct tatcgtcatc
     8341 gtctttgtaa tcaatatcat gatccttgta gtctccgtcg tggtccttat agtccatggt
     8401 ggcaccggtc caacctgaaa aaaagtgatt tcaggcaggt gctccaggta attaaacatt
     8461 aataccccac caaccaacca tcccttaaac ccttacctct tgctcagcta attacagccc
     8521 ggaggagaag ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg
     8581 ggcgggcggc gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact
     8641 cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa
     8701 ggaaactttc ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg
     8761 ccccgccc
//
