LOCUS       sequence-33952-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pDONR221 - myc ULK1 k46I
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             99..128
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct tcaccatgga acaaaagcta atctccgagg
      121 aagacttggg aggaatagag ccgggccgag gcggcgtcga gaccgtgggc aagttcgagt
      181 tctctcgcaa ggacctgatt ggacacggcg ccttcgcggt ggtcttcaag ggtcgacacc
      241 gcgagaagca cgacctggag gtggccgtca tatgcattaa caagaagaac cttgccaagt
      301 cccaaacact gctgggaaag gaaatcaaaa tcctgaagga actaaagcac gaaaacatcg
      361 tggcgctgta tgacttccag gaaatggcta attctgtcta cctggtcatg gagtattgta
      421 atggtggaga cctggctgac tacctgcaca ctatgcgcac actgagtgaa gacactgtca
      481 ggcttttcct acagcagatc gctggcgcca tgcggctgct gcacagcaag ggcatcatcc
      541 accgggacct gaagccccaa aacatcctgc tgtccaaccc tgggggccgc cgggccaacc
      601 ccagcaacat ccgagtcaag attgctgact ttggattcgc tcggtacctc cagagcaaca
      661 tgatggcggc cacactctgt ggttctccta tgtacatggc tcctgaggtc attatgtccc
      721 agcactacga tggaaaggct gacctgtgga gcattggcac cattgtctac cagtgtctga
      781 cagggaaggc cccttttcag gccagcagcc ctcaggattt gcgcctgttt tatgag
//
