LOCUS       sequence-339527-       12425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For light regulated release of GluA1 from the endoplasmic reticulum.
            Driven by synapsin promoter. Thrombin site is included between GFP 
            and GluA1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Bourke AM, Schwartz SL, Bowen AB, Kleinjan MS, Winborn CS, Kareemo 
            DJ, Gutnick A, Schwarz TL, Kennedy MJ
  TITLE     zapERtrap: A light-regulated ER release system reveals unexpected 
            neuronal trafficking pathways.
  JOURNAL   J Cell Biol. 2021 Sep 6;220(9). pii: 212461. doi: 
            10.1083/jcb.202103186. Epub 2021 Jul 9.
  PUBMED    34241635
REFERENCE   2  (bases 1 to 12425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-09-6"; volume: "220(9). pii: 212461. doi"; 
            pages: " 10.1083/jcb.202103186. Epub 2021 Jul 9"
COMMENT     Sequence Label: DHFR-GFP-th-GluA1
FEATURES             Location/Qualifiers
     source          1..12425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2395..2842
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2958..3431
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             join(3453..3455,3456..4166)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3453..3455,3456..4166)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3495..3516)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3756..3775)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4103..4124
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4167..4184
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     misc_feature    6880..7468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6933..6953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7351..7362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7471..7487)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7472..7488)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7993..8173
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8199..8216)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8205..8429
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8475..8903
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8562..8581)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8772..8793
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8912..8932)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8917..9246
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9097..9232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9159..9178
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9294..9341
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9360..9734
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9864..9985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9901..9920)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9955..9974
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10034..10050)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10034..10050)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10047..10069)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10058..10074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10082..10088,10089..10106,10107..10112))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10127..10148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10265..10282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10436..11024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10516..10535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11195..11986,11987..12055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11818..11837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12056..12160)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12235..12254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaagtgtct agactgcaga gggccctgcg tatgagtgca
     2401 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
     2461 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
     2521 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
     2581 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
     2641 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
     2701 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
     2761 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
     2821 cgtgtcgtgc ctgagagcgc agtcgagaaa ccaccatgcc gtacatcttt gcctttttct
     2881 gcaccggttt tctaggtgcg gttgtgggtg ccaatgcgcg aggtggaggc ggtggaagag
     2941 ggggtggagg aacgcgtatc agtctgattg cggcgttagc ggtagatcgc gttatcggca
     3001 tggaaaacgc catgccgtgg aacctgcctg ccgatctcgc ctggtttaaa cgcaacacct
     3061 taaataaacc cgtgattatg ggccgccata cctgggaatc aatcggtcgt ccgttgccag
     3121 gacgcaaaaa tattatcctc agcagtcaac cgggtacgga cgatcgcgta acgtgggtga
     3181 agtcggtgga tgaagccatc gcggcgtgtg gtgacgtacc agaaatcatg gtgattggcg
     3241 gcggtcgcgt ttatgaacag ttcttgccaa aagcgcaaaa actgtatctg acgcatatcg
     3301 acgcagaagt ggaaggcgac acccatttcc cggattacga gccggatgac tgggaatcgg
     3361 tattcagcga attccacgat gctgatgcac agaactctca cagctattgc tttgagattc
     3421 tggagcggcg gtctgccgga tccgcagagc aggtgagcaa gggcgaggag ctgttcaccg
     3481 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3541 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3601 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3661 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3721 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3781 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3841 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3901 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3961 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4021 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4081 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4141 ctctcggcat ggacgagctg tataagcttg ttcctcgagg aagcagtgct ggtagtttcc
     4201 ccaacaatat ccagataggg gggttatttc caaaccaaca atcacaggaa catgcggctt
     4261 ttaggtttgc tttgtcacaa ctcacggagc cccccaagct gcttccccag atcgatattg
     4321 tgaacatcag cgacagcttt gagatgactt accgtttctg ttcccagttc tccaaaggag
     4381 tctatgccat ctttggattt tatgaacgaa ggactgtcaa catgctgacc tccttctgtg
     4441 gggccctcca tgtgtgcttc attactccaa gttttcctgt tgacacatcc aatcaatttg
     4501 tccttcagct acgcccggaa ctacaggaag ctctcattag cattatcgac cattacaagt
     4561 ggcaaacctt tgtctacatt tatgatgctg accggggcct gtcagtcctg cagagagtct
     4621 tggatacagc cgcagagaag aactggcagg taacggctgt caacattctg acaaccaccg
     4681 aggaaggata ccggatgctc tttcaggacc tggagaagaa aaaggagagg ctggtggtgg
     4741 ttgactgtga atcagaacgc ctcaacgcca tcctgggcca gatcgtgaag ctagaaaaga
     4801 atggcatcgg gtaccactac atcctcgcca atctgggctt catggacatt gacttaaata
     4861 agttcaagga gagcggagcc aatgtgacag gtttccagct ggtgaactac acagacacga
     4921 tcccagccag aatcatgcag caatggagga caagtgactc ccgagaccat accagggtgg
     4981 actggaagag gccaaagtac acttctgctc tcacctatga tggtgtcaag gtgatggctg
     5041 aggccttcca aagcctgcgg aggcagagga ttgacatatc ccgccggggg aatgctgggg
     5101 actgtctggc taacccagct gtgccctggg gtcaagggat cgacatccag agagccctgc
     5161 agcaggtgcg cttcgaaggt ttgacaggaa atgtgcagtt caacgagaaa gggcgccgga
     5221 ccaattacac cctccacgtg atcgaaatga aacatgatgg aatccgaaag attggttact
     5281 ggaatgaaga cgataaattt gtccccgcag ccaccgacgc tcaggctgga ggggacaact
     5341 caagcgtcca gaataggacc tacatcgtca ctactatcct cgaagatcct tacgtgatgc
     5401 ttaaaaagaa tgccaaccag tttgagggca atgaccgcta tgagggctac tgtgtggagc
     5461 tggctgcaga gatcgccaag cacgtgggct actcctaccg acttgagatt gtcagcgacg
     5521 gcaaatatgg agcccgggat cccgacacaa aggcttggaa tggcatggtg ggagaactgg
     5581 tctatggaag agcagacgtg gctgtggctc ccttgaccat aaccttggtc cgggaggaag
     5641 tcatcgactt ctccaagcca ttcatgagtt tgggaatctc cattatgatt aagaagccac
     5701 agaagtccaa gccaggtgtc ttctcctttc ttgacccttt ggcctatgag atctggatgt
     5761 gtatagtgtt tgcctacatt ggagtgagcg tcgtcctctt cctggtcagc cgtttcagcc
     5821 cctacgaatg gcacagcgaa gagtttgaag agggacgaga ccagacaacc agtgaccagt
     5881 caaatgagtt tggcatattc aacagcctgt ggttctccct gggggccttc atgcagcaag
     5941 gatgtgacat ttcccccagg tccctgtccg gacgcatcgt cggcggcgtc tggtggttct
     6001 tcactttgat catcatctcc tcgtacacag ccaacctggc tgccttcctg actgtggaga
     6061 ggatggtgtc tcccattgag agtgcagagg acctggcaaa gcagacggaa attgcttatg
     6121 ggacattgga agcaggctcc actaaggagt tcttcaggag atctaaaatc gctgtgtttg
     6181 agaagatgtg gacatacatg aagtctgcag aaccatccgt gtttgttcgg accacagagg
     6241 aaggcatgat cagagtgaga aaatctaaag gcaaatacgc ctacctcctg gagtccacca
     6301 tgaatgagta tattgagcaa cgaaagccct gtgacaccat gaaagtggga ggtaacttgg
     6361 attccaaagg ctatggcatt gcgacaccca aggggtccgc cctgagaaat ccagtaaacc
     6421 tggcagtgtt aaaactgaac gagcaggggc ttttggacaa attgaaaaac aaatggtggt
     6481 acgacaaggg cgagtgcggc agcgggggag gtgactccaa ggacaagacc agcgctttga
     6541 gcctcagcaa tgtggcaggc gtgttctaca tcctgattgg agggctggga ctggccatgc
     6601 tggttgcctt aatcgagttc tgctacaaat cccgtagcga gtcgaagcgg atgaagggtt
     6661 tctgtttgat cccacagcaa tccatcaatg aagccatacg gacatcgacc ctcccccgga
     6721 acagtggggc aggagccagc ggaggaggcg gcagtggaga gaatggccgg gtggtcagcc
     6781 aggacttccc caagtccatg caatccattc cctgcatgag tcacagttca gggatgccct
     6841 tgggagccac aggattgtaa cgatatcaag cttatcgata atcaacctct ggattacaaa
     6901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7261 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7381 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7441 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     7501 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     7561 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     7621 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7681 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7741 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     7801 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     7861 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     7921 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     7981 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8041 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8101 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8161 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8221 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8281 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8341 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8401 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8461 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8521 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8581 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8641 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8701 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8761 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8821 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8881 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     8941 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9001 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9061 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9121 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9181 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9241 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9301 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9361 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9421 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     9481 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     9541 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     9601 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     9661 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     9721 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     9781 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     9841 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     9901 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9961 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    10021 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10081 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10141 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10201 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10261 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10321 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10381 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10441 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10501 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10561 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10621 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10681 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10741 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10801 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10861 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10921 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10981 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11041 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11101 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11161 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11221 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11281 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11341 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11401 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11461 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11521 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11581 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11641 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11701 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11761 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11821 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11881 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11941 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12001 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12061 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12121 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12181 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
    12241 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
    12301 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
    12361 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    12421 gcgtt
//
