LOCUS       sequence-33954-e         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pQCXIN- myc ULK1 K46I
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(443..472)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(481..505)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    546..564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    567..585
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gcaggatgtt ttggggcttc aggtcccggt ggatgatgcc cttgctgtgc agcagccgca
       61 tggcgccagc gatctgctgt aggaaaagcc tgacagtgtc ttcactcagt gtgcgcatag
      121 tgtgcaggta gtcagccagg tctccaccat tacaatactc catgaccagg tagacagaat
      181 tagccatttc ctggaagtca tacagcgcca cgatgttttc gtgctttagt tccttcagga
      241 ttttgatttc ctttcccagc agtgtttggg acttggcaag gttcttcttg ttaatgcata
      301 tgacggccac ctccaggtcg tgcttctcgc ggtgtcgacc cttgaagacc accgcgaagg
      361 cgccgtgtcc aatcaggtcc ttgcgagaga actcgaactt gcccacggtc tcgacgccgc
      421 ctcggcccgg ctctattcct cccaagtctt cctcggagat tagcttttgt tccatggtga
      481 agcctgcttt tttgtacaaa cttgttgata tctgcagaat tccaccacac tggactagtc
      541 gacgatctct atcactgata gggagatctc tatcactgat agggagagct ct
//
