LOCUS       sequence-339545-        5396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3
            - EGFP fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Schanda K, Peschl P, Lerch M, Seebacher B, Mindorf S, Ritter N, 
            Probst M, Hegen H, Di Pauli F, Wendel EM, Lechner C, Baumann M, 
            Mariotto S, Ferrari S, Saiz A, Farrell M, Leite MIS, Irani SR, 
            Palace J, Lutterotti A, Kumpfel T, Vukusic S, Marignier R, Waters P,
            Rostasy K, Berger T, Probst C, Hoftberger R, Reindl M
  TITLE     Differential Binding of Autoantibodies to MOG Isoforms in 
            Inflammatory Demyelinating Diseases.
  JOURNAL   Neurol Neuroimmunol Neuroinflamm. 2021 Jun 15;8(5). pii: 8/5/e1027. 
            doi: 10.1212/NXI.0000000000001027. Print 2021 Jul.
  PUBMED    34131067
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurol 
            Neuroimmunol Neuroinflamm."; date: "2021-06-15"; volume: "8(5). pii:
            8/5/e1027. doi"; pages: " 10.1212/NXI.0000000000001027. Print 2021 
            Jul"
COMMENT     Sequence Label: pEGFP-N1 hMOG-alpha3-EGFP
FEATURES             Location/Qualifiers
     source          1..5396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..249
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     206..226
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(251..355)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      382..837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(469..488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     679..700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    844..965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(855..874)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     909..928
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(1088..1801,1802..1804,1805..1807))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1091..1801,1802..1804,1805..1807))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1133..1154)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1482..1501
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1741..1762
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1804..1813
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2581..2784)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2610..2630)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2785..3088
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3263..3851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3343..3362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4180..4227
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4252..4271
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4459..5253)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4459..5253)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4570..4589)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5180..5199
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(5288..5396,1..249))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5302..5396,1..41)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
       61 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
      121 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
      181 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt
      241 ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      361 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg
      841 ccttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      901 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      961 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1021 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
     1081 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     1141 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     1201 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     1261 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     1321 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     1381 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     1441 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     1501 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     1561 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     1621 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     1681 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     1741 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     1801 tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcgga
     1861 agggatttcg tagctcttca aggaattgcc ctgtcttttt tggttcctta acctggggtc
     1921 ctagaacacc aaaggactca aaagtccggt ggagattctc tatctctgct cgaagttttc
     1981 ctctcagtct gtactgcagg cagaggaaga tgaggccaac agtgatctgc aggaggagca
     2041 caggcagcac cgcgaggaga accagcactc cagggctcac ccagtagaaa ggatcttcta
     2101 ctttcaattc cattgctgcc tcctcttggt aagaatgatc tcggaagaag caggtgaaac
     2161 ctccttcatc tgagaacctt acattccgga tcctgagagt cacctttccc tcaccaatag
     2221 catctttcag cagctctgtc cggccccgat attcaggtgc ctggtctcca tcttggtcct
     2281 tgccatttct gtagagatga accaccctag agaagggggg gcggtaccac cccacctcca
     2341 tgcctgtagc gttcttccca ggagatatgc gacatggcaa ttccacttca tccccgacca
     2401 gagcccggat agggtgtctt ggtcctatca ctctgaactg ccctgcatag ctggaagaca
     2461 cttggaggag gaggaggagg aggaaggagc agaggcagct gggcagagag ggtcttgata
     2521 agcttgccat ctcgagatct gagtccggta gcgctagcgg atctgacggt tcactaaacc
     2581 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     2641 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
     2701 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
     2761 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
     2821 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     2881 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     2941 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     3001 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     3061 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     3121 ccccgtaatt gattactatt aataactaat gcatggcggt aatacggtta tccacagaat
     3181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg
     4021 cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt
     4081 ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat
     4141 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt
     4201 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct
     4261 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc
     4321 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     4381 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     4441 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     4501 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     4561 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     4621 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     4681 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc
     4741 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     4801 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     4861 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     4921 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     4981 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     5041 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
     5101 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     5161 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     5221 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     5281 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     5341 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatgg
//
