LOCUS       sequence-339550-       10658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CCR6 hLOV receiver construct in DIPG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Cho KF, Gillespie SM, Kalogriopoulos NA, Quezada MA, Jacko M, Monje 
            M, Ting AY
  TITLE     A light-gated transcriptional recorder for detecting cell-cell 
            contacts.
  JOURNAL   Elife. 2022 Mar 21;11:e70881. doi: 10.7554/eLife.70881.
  PUBMED    35311648
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-21"; volume: "11:e70881. doi"; pages: " 
            10.7554/eLife.70881"
COMMENT     Sequence Label: pPB EF1a CCR6-hLOV-TEVcs-Gal4
FEATURES             Location/Qualifiers
     source          1..10658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1367..1484
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1531..1742
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1755..2023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        2048..2110
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             3737..3757
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             3770..3793
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3794..4231
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4232..4576
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    4592..5180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4645..4665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5063..5074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5187..6398
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6235..6254
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6421..7215
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6421..7215
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6475..6494)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7085..7104
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7228..7349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7265..7284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7319..7338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8112..8146
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8637..8653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8637..8653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8650..8672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8661..8677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8685..8691,8692..8709,8710..8715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8730..8751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8868..8885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9039..9627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9119..9138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9798..10589,10590..10658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10421..10440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagaactttt aaaagaaaag
     1381 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1441 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta
     1501 gcctcgaggg atcatccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     1561 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
     1621 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     1681 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     1741 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     1801 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     1861 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     1921 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     1981 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
     2041 cgaattcttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg
     2101 acccgcttaa aagatccagc caccaccggt cgccaccgcc accatgagcg gagagagcat
     2161 gaacttcagt gatgtgttcg attccagtga ggactacttc gtgtccgtca atacatccta
     2221 ctacagcgta gattctgaga tgctgctttg ctccctgcag gaggtgcgcc agttctcccg
     2281 actcttcgtt cccatcgcgt attcactcat ctgcgtcttt ggcctcctgg ggaatatctt
     2341 ggtagtcatc acatttgctt tttataaaaa ggccagatcc atgacagacg tctacctgct
     2401 taatatggcc attgcagaca tcctgttcgt cctgacgctc ccattctggg ctgtgagtca
     2461 cgctaccggg gcctgggtgt tcagtaatgc aacctgcaag ctgttgaagg gaatttatgc
     2521 catcaacttt aattgcggta tgctgctgct gacttgcata agtatggaca gatacatcgc
     2581 catagtacag gcaacaaaat ccttcagact gaggtctcga acgctcccta ggtctaaaat
     2641 catttgtctt gttgtatggg gcttgagcgt gattatttcc agcagtactt tcgtgtttaa
     2701 ccagaagtac aacacgcagg gcagcgatgt gtgtgagccc aagtaccaga cggtatctga
     2761 gcctatcagg tggaagctgt tgatgctggg tttggaattg ctgttcggct tttttatccc
     2821 actcatgttt atgatttttt gttatacctt catcgtgaag accctggtcc aggcacaaaa
     2881 ctctaagcga cacaaggcca tccgggtaat catcgctgtt gtgctggtat ttctggcttg
     2941 ccagatccct cataatatgg tccttctggt aacggcagcc aacctgggca aaatgaatcg
     3001 ctcatgtcag tcagaaaagc ttatcgggta caccaaaacg gttacggagg ttctggcgtt
     3061 cctgcattgt tgccttaacc ctgtgctgta cgcatttata ggtcagaagt ttcgaaacta
     3121 cttcctgaag attctcaaag acctctggtg tgtaagaaga aagtataaaa gtagtggatt
     3181 ttcatgcgca ggacgctact ccgaaaacat ttccagacag accagtgaga cggccgataa
     3241 cgataacgcc tcctccttta ccatgactag tgagctggcc gagaagctgg ccggcctgga
     3301 catcaacggt ggagctagcg agtttcgggc aaccacactg gaacggatcg agaaaagctt
     3361 cgtgattact gatccgagac tgcctgacaa cccaatcatt tttgtgagcg attccttcct
     3421 gcagctgaca gaatattctc gggaagagat cctggggcgc aattgccgtt ttctgcaggg
     3481 acccgagaca gaccgtgcca ctgttcggaa aatcagagat gctattgaca accagactga
     3541 agtgaccgtt cagctgatca attataccaa gagcggcaag aagttctgga acgtgttcca
     3601 cctgcagccg atgcgcgatt ataagggcga cgtccagtac ttcattggcg tgcagctgga
     3661 tggcaccgaa cgtcttcatg gcgccgctga gcgtgaggcg gtctgcctgg tcaaaaagac
     3721 agcctttcag attgctgaaa atctctactt ccagatgggc ggaggatccg actacaagga
     3781 tgacgacgat aagaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa
     3841 aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga
     3901 gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt
     3961 ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct
     4021 tgacatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt
     4081 tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga
     4141 tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag
     4201 taacaaaggt caaagacagt tgactgtatc ggccaatttt aatcaaagtg ggaatattgc
     4261 tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac
     4321 tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc atgataactt
     4381 catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc
     4441 acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag ggatgtttaa
     4501 taccactaca atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa
     4561 cccaaaaaaa gagtaaagcg gccgcgtcga caatcaacct ctggattaca aaatttgtga
     4621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4981 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5161 cctttgggcc gcctccccgc ggatccggcc tccgcgccgg gttttggcgc ctcccgcggg
     5221 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     5281 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     5341 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     5401 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     5461 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     5521 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     5581 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     5641 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     5701 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     5761 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     5821 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     5881 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     5941 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     6001 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     6061 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     6121 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     6181 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     6241 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     6301 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     6361 ccgctaaatt ctggccgttt ttggcttttt tgttagacgc tagcggattc tagagccacc
     6421 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6481 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6541 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6601 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6661 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6721 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6781 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6841 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6901 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     6961 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7021 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7081 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     7141 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     7201 gacgagttct tctgagttaa ctaactaaac ttgtttattg cagcttataa tggttacaaa
     7261 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7321 ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa
     7381 ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc ctgtttaata
     7441 tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc aaccaggttg
     7501 cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag ccaggtttag
     7561 ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt ccccgtatcc
     7621 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
     7681 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
     7741 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
     7801 attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca agaaaatata
     7861 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     7921 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     7981 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     8041 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     8101 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca
     8161 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     8221 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     8281 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     8341 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     8401 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     8461 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     8521 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     8581 gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca
     8641 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8701 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8761 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8821 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8881 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8941 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9661 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9721 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9781 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9841 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9901 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9961 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10021 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10081 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10141 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10201 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10261 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10321 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10381 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10441 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10501 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10561 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10621 aagggaataa gggcgacacg gaaatgttga atactcat
//
