LOCUS       sequence-339559-       12686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a modularized single-plasmid glycosylase base editing (C-to-G) tool 
            for Sinorhizobium meliloti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12686)
  AUTHORS   Wang L, Xiao Y, Wei X, Pan J, Duanmu D
  TITLE     Highly Efficient CRISPR-Mediated Base Editing in Sinorhizobium 
            meliloti.
  JOURNAL   Front Microbiol. 2021 Jun 18;12:686008. doi: 
            10.3389/fmicb.2021.686008. eCollection 2021.
  PUBMED    34220774
REFERENCE   2  (bases 1 to 12686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-06-18"; volume: "12:686008. doi"; pages: " 
            10.3389/fmicb.2021.686008. eCollection 2021"
COMMENT     Sequence Label: pKm-HGBE
FEATURES             Location/Qualifiers
     source          1..12686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1423..1868)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     terminator      1869..1940
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_RNA        complement(1998..2073)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(2115..2420)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2217..2236)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2762..3421)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3336..3355
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3475..3481,3482..3499,3500..3505))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(3616..4239)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(join(4453..8526,8527..8529,8530..8556))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      9696..9790
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9889..9908)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9900..10715
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            10866..10974
                     /note="incP origin of transfer"
     CDS             complement(10985..11647)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      11648..12419
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(12524..12610)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttatttatta actgttaatt gtccttgttc aaggatgctg tctttgacaa cagatgtttt
       61 cttgcctttg atgttcagca ggaagctcgg cgcaaacgtt gattgtttgt ctgcgtagaa
      121 tcctctgttt gtcatatagc ttgtaatcac gacattgttt cctttcgctt gaggtacagc
      181 gaagtgtgag taagtaaagg ttacatcgtt aggatcaaga tccattttta acacaaggcc
      241 agttttgttc agcggcttgt atgggccagt taaagaatta gaaacataac caagcatgta
      301 aatatcgtta gacgtaatgc cgtcaatcgt catttttgat ccgcgggagt cagtgaacag
      361 ataccatttg ccgttcattt taaagacgtt cgcgcgttca atttcatctg ttactgtgtt
      421 agatgcaatc agcggtttca tcactttttt cagtgtgtaa tcatcgttta gctcaatcat
      481 accgagagcg ccgtttgcta actcagccgt gcgtttttta tcgctttgca gaagtttttg
      541 actttcttga cggaagaatg atgtgctttt gccatagtat gctttgttaa ataaagattc
      601 ttcgccttgg tagccatctt cagttccagt gtttgcttca aatactaagt atttgtggcc
      661 tttatcttct acgtagtgag gatctctcag cgtatggttg tcgcctgagc tgtagttgcc
      721 ttcatcgatg aactgctgta cattttgata cgtttttccg tcaccgtcaa agattgattt
      781 ataatcctct acaccgttga tgttcaaaga gctgtctgat gctgatacgt taacttgtgc
      841 agttgtcagt gtttgtttgc cgtaatgttt accggagaaa tcagtgtaga ataaacggat
      901 ttttccgtca gatgtaaatg tggctgaacc tgaccattct tgtgtttggt cttttaggat
      961 agaatcattt gcatcgaatt tgtcgctgtc tttaaagacg cggccagcgt ttttccagct
     1021 gtcaatagaa gtttcgccga ctttttgata gaacatgtaa atcgatgtgt catccgcatt
     1081 tttaggatct ccggctaatg caaagacgat gtggtagccg tgatagtttg cgacagtgcc
     1141 gtcagcgttt tgtaatggcc agctgtccca aacgtccagg ccttttgcag aagagatatt
     1201 tttaattgtg gacgaatcaa attcagaaac ttgatatttt tcattttttt gctgttcagg
     1261 gatttgcagc atatcatggc gtgtaatatg ggaaatgccg tatgtttcct tatatggctt
     1321 ttggttcgtt tctttcgcaa acgcttgagt tgcgcctcct gccagcagtg cggtagtaaa
     1381 ggttaatact gttgcttgtt ttgcaaactt tttgatgttc atcgttcatg tctccttttt
     1441 tatgtactgt gttagcggtc tgcttcttcc agccctcctg tttgaagatg gcaagttagt
     1501 tacgcacaat aaaaaaagac ctaaaatatg taaggggtga cgccaaagta tacactttgc
     1561 cctttacaca ttttaggtct tgcctgcttt atcagtaaca aacccgcgcg atttactttt
     1621 cgacctcatt ctattagact ctcgtttgga ttgcaactgg tctattttcc tcttttgttt
     1681 gatagaaaat cataaaagga tttgcagact acgggcctaa agaactaaaa aatctatctg
     1741 tttcttttca ttctctgtat tttttatagt ttctgttgca tgggcataaa gttgcctttt
     1801 taatcacaat tcagaaaata tcataatatc tcatttcact aaataatagt gaacggcagg
     1861 tatatgtgca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1921 gtttgtcggt gaacgctctc tctagatact agagtcacac tggcccgggc catgggtatg
     1981 gacagatctc aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
     2041 cttattttaa cttgctattt ctagctctaa aactgagacc ctgcagactg gctgtgtata
     2101 agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
     2161 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg acaccggcac actggccata
     2221 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
     2281 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
     2341 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
     2401 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat
     2461 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
     2521 acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc
     2581 atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
     2641 atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
     2701 aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccagatc
     2761 tttacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2821 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2881 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2941 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     3001 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     3061 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     3121 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     3181 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     3241 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     3301 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     3361 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     3421 ttttagcttc cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat
     3481 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgcggccgcg gtctccaggc
     3541 aggcacatac cgtgtcgacc caatcgtgtc aaccgcgtgc cggggatttg tttaaactca
     3601 gccgctgccg cgcgagacgg ccgggctctt cgtggtgtgg aggatcttga cctggatcat
     3661 gatgctcagc tcgctgcggc gcttctccgc gcgcttcagg gtcttctcca gccagcggtt
     3721 ctcgttgagc tggttatggc tcgactggat gaagatcttg cggcagcact ggtagtgctc
     3781 gctgaccatg acgttcaggc cgacgccgtt gtcgcggagg ttccacaggc cgatctggtt
     3841 gcgcgcgttc ttctcgtaat agagcttgca ggcccagatc ttcagggtat ggccgttgcc
     3901 gcggagctcc tggttatacc actccaggat cttctcggcg cagtccgcgc acggcgacca
     3961 cgacgaatac cagttgatcg tgaactggcc cgggttgtcg cggagatact cctcgacctt
     4021 gcggatgctg aagatctcgg cgtggatgcc gcgctcggtg ccgctctgcg gcttgttgac
     4081 cgcgtagccc cagaagcagg cgcgacgctc gccgcggcgc ttgagctcga agaggacata
     4141 gcagcgatgg ctgaccgact tcttgttgtt gaagaactgc ttcttgaacg tgtagatgtc
     4201 cagcttctcg tggatgcgga catactccgc gtcggtcatg cggctgcccg agtacttctc
     4261 gacatacggg accgggatca tgccgcgctt gccctcgctg tcctccgcgt tccaccactg
     4321 ctcctccggc ttgtcgcgga tgcggaggat gtcgcccttc ttgaacggca gatcttcttc
     4381 gtcgttgccg ttgaagtcga acagcgcgcg gacatactcg gccgagccgc cgccgcccga
     4441 gccgccgcca ccgtcgccgc ccagctggct caggtcgatg cgcgtctcgt agaggccggt
     4501 gatcgactga tggatgagcg tcgcgtccag gacttccttc gtcgaggtgt agcgcttgcg
     4561 gtcgatcgtg gtgtcgaaat acttgaacgc ggccggggcg cccaggttgg tcagcgtgaa
     4621 gagatggatg atgttctccg cctgctcgcg gatcggcttg tcgcgatgct tgttgtaggc
     4681 gctgaggacc ttgtccaggt tcgcgtcggc caggatgacg cgcttgctga actccgagat
     4741 ctgctcgatg atctcgtcga gataatgctt gtgctgctcg acgaacagct gcttctgctc
     4801 gttgtcctcc gggctgccct tgagcttctc gtagtgcgac gccagataga ggaagttgac
     4861 gtacttcgac ggcagggcga gctcgttgcc cttctggagc tcgccggcgc tagcgagcat
     4921 gcgcttgcgg ccgttctcca gctcgaagag cgagtacttc gggagcttga tgatcaggtc
     4981 cttcttgact tccttatagc ccttcgcctc caggaagtcg atcgggttct tctcgaacga
     5041 cgagcgctcc atgatcgtga tgccgagcag ctccttgacg ctcttgagct tcttcgactt
     5101 gcccttctcg accttcgcga cgaccaggac cgagtaggcg accgtcgggc tgtcgaagcc
     5161 accatacttc ttcgggtccc agtccttctt gcgggcgatg agcttgtccg agttgcgctt
     5221 cggcaggatg ctctccttcg agaagccgcc cgtctggacc tcggtcttct tgacgatgtt
     5281 gacctgcggc atgctgagga ccttgcggac cgtggcgaag tcgcggccct tgtcccagac
     5341 gatctcgccc gtctcgccgt tcgtctcgat gagcgggcgc ttgcggatct cgccgttggc
     5401 gagggtgatc tcggtcttga agaagttcat gatgttcgaa tagaagaagt acttcgccgt
     5461 ggccttgccg atctcctgct cgctcttggc gatcatcttg cggacgtcat agaccttgta
     5521 gtcgccatag acgaactccg actccagctt cgggtacttc ttgatcagcg cggtgccgac
     5581 gaccgcgttg aggtaggcgt catgggcatg gtgatagttg ttgatctcgc ggaccttata
     5641 gaactggaag tccttgcgga agtcgctgac gagcttcgac ttcagcgtga tgaccttgac
     5701 ctcgcggatc agcttgtcgt tctcgtcata cttggtgttc atgcgcgagt cgaggatctg
     5761 cgcgacgtgc ttcgtgatct ggcgcgtctc gaccagctgg cgcttgatga agcccgcctt
     5821 gtccagctcc gagaggccgc cgcgctccgc cttggtgagg ttgtcgaact tgcgctgggt
     5881 gatcagcttg gcgttgagca gctggcgcca gtagttcttc atcttcttga cgacttcctc
     5941 gctcgggacg ttgtccgact tgccgcggtt cttgtccgag cgcgtgagga ccttgttgtc
     6001 gatgctgtcg tccttcagga acgactgcgg gacgatatgg tcgacgtcat agtcgctgag
     6061 gcggttgatg tccagctcct ggtcgacgta catgtcgcgg ccgttctgga ggtaatacag
     6121 atagagcttc tcgttctgca gctgggtgtt ctcgaccggg tgctccttga ggatctgcga
     6181 gcccagctcc ttgatgcctt cctcgatgcg cttcatgcgc tcgcggctgt tcttctggcc
     6241 cttctgcgtg gtctggttct cgcgggccat ctcgatgacg atgttctccg gcttatggcg
     6301 gcccatgacc ttgacgagct cgtcgacgac cttgacggtc tgcaggatgc ccttcttgat
     6361 ggccggcgag ccggccaggt tggcgatgtg ttcgtgcagg ctgtcgccct ggccgctgac
     6421 ctgggccttc tggatgtctt ccttgaacgt gaggctgtcg tcgtggatca gctgcatgaa
     6481 gttgcggttg gcgaagccgt cgctcttcag gaagtcgagg atggtcttgc ccgactgctt
     6541 gtcgcggatg ccgttgatga gcttgcgcga caggcggccc cagccggtgt agcggcgacg
     6601 cttcagctgc ttcatgacct tgtcgtcgaa gaggtgggca taggtcttca ggcgctcctc
     6661 gatcatctcg cggtcctcga acagcgtgag ggtcaggacg atgtcctcca ggatatcctc
     6721 gttctcctcg ttgtcgagga agtccttgtc cttgatgatc ttgagcaggt catgataggt
     6781 gcccaggctg gcgttgaagc ggtcctcgac gccgctgatc tcgaccgagt cgaagcactc
     6841 gatcttcttg aagtagtctt ccttcagctg cttgaccgtg accttgcggt tggtcttgaa
     6901 gagcaggtcg acgatcgcct tcttctgctc gccgctgagg aaggccggct tgcgcatgcc
     6961 ttcggtgaca tacttgacct tcgtcagctc gttgtagacg gtgaagtact catagagcag
     7021 gctgtgcttc gggaggacct tctcgttcgg caggttcttg tcgaagttcg tcatgcgctc
     7081 gatgaagctc tgggcgctgg cgcccttgtc gacgacttcc tcgaagttcc acggcgtgat
     7141 cgtctcctcg ctcttgcggg tcatccacgc gaagcgcgag ttgccgcgtg ccagcgggcc
     7201 gacataatac gggatgcgga acgtcaggat cttctcgatc ttctcgcggt tgtccttgag
     7261 gaacggatag aagtcttcct ggcgacgcag gatcgcatgc agctcgccga gatggatctg
     7321 gtgcgggatc gagccgttgt cgaaggtgcg ctgcttgcgg agcaggtcct cgcggttgag
     7381 cttgacgagc agctcctccg tgccgtccat cttctccagg atcggcttga tgaacttgta
     7441 gaactcttcc tggcttgcgc cgccgtcgat gtagcccgca tagccgttct tgctctggtc
     7501 gaagaagatc tccttatact tctccgggag ctgctggcgg accagggcct tcaggagcgt
     7561 gaggtcctga tggtgctcgt cgtagcgctt gatcatgctc gccgacagcg gggccttcgt
     7621 gatctcggtg ttgacgcgga ggatgtcgct gagcaggatc gcgtccgaca ggttcttcgc
     7681 ggccaggaag aggtccgcat actggtcgcc gatctgggcg agcaggttgt cgaggtcgtc
     7741 gtcgtaggtg tccttcgaca gctggagctt cgcgtcctcg gccaggtcga agttgctctt
     7801 gaagttcggg gtcaggccca ggctcagggc gatgaggttg ccgaagaggc cgttcttctt
     7861 ctcgcccggg agctgggcga tcaggttctc caggcggcgc gacttgctca ggcgggccga
     7921 gaggatggcc ttggcgtcga cgccgctggc gttgatcggg ttctcctcga acagctggtt
     7981 atacgtctgg accagctgga tgaagagctt gtcgacgtcc gagttgtccg ggttgaggtc
     8041 gccctcgatc aggaaatggc cgcggaactt gatcatgtgc gcgagggcca ggtagatgag
     8101 gcgcaggtcg gccttgtcgg tgctgtcgac cagcttcttg cggagatgat agatcgtcgg
     8161 gtacttctcg tgatacgcga cctcgtcgac gatgttgccg aagatcggat ggcgctcgtg
     8221 cttcttatct tcttcgacga ggaagctctc ctccaggcga tggaagaacg agtcgtcgac
     8281 cttggccatc tcgttcgaga agatctcctg gagatagcag atgcggttct tgcgacgcgt
     8341 gtagcggcga cgggcggtgc gcttcaggcg ggtggcctcc gccgtctcgc cgctgtcgaa
     8401 cagcagggcg ccgatgaggt tcttcttgat gctgtggcgg tcggtgttgc ccaggacctt
     8461 gaacttcttc gacgggacct tatactcgtc ggtgatgacc gcccagccga cgctgttcgt
     8521 gccgatggcc aggccgatgc tgtacttctt gtccatctcc gtgcccggcg tctccgagcc
     8581 gctgagctcc ttccagtcga tcggcttctt gccgctcttc tgcaggagct cgttcgtctt
     8641 cgagaaatgg cggcagccga agaagccgcg gtagaccgac agcggcgacg ggtgggccgt
     8701 ctgcaggaca tggtggcgct tgcggtcgat ggccgagccc ttcttctggg catacgagcc
     8761 ccacaggagg aagaccaggc cgttgctgtt ctggttgagc cacgagacga cggcgtcggt
     8821 gaactgctcc cagccgcgct ccttatggct gttggcctgg tgggcgcgga ccgtgaggac
     8881 ggcgttcagg agcaggacgc cctgcttggc ccagccgctg aggtcgccat ggcccggatg
     8941 gacgaagtcc tcgatgtccg tgctcagctc cttgtagatg ttctccagcg acggcggcgg
     9001 cgggaccggg cgctggacgc tgaagcacag gccatgggcc tggttcgggc catgatacgg
     9061 gtcctggccg aggatgacga ccttgacgtc cttgatgtcg cacatctggg tccaggtgaa
     9121 gacctggtgc ggcggcggat agacggtata gtgcttgcgc tcctcggcga cgaagcccat
     9181 cagcttgatg aaatacggct tgccgaactc gccgctgagg tgcttcttcc acgactcgcc
     9241 gaacatctcg agctccagtg ccctgtcgcc tttcttcctt caaagcgatc agtggtcaac
     9301 cccggacagg ctccgacgcg cgggctgcgg caaaaggcct ctcggcccca gacctcggtc
     9361 attctgcgcg gcgcgtgtcg tgcctggcaa gcaggaaagg cgatggaaaa tgacgacaca
     9421 ttttcaaacc ttccgccgta atcaggcgcg gcatcacacc gagtccgagt gcccagtttt
     9481 gaaccattcc aaaatggctg gcaaaacttc attctcccga acatcgatcg aagtcaagca
     9541 attgcggaaa aaggacggcc gctgccgccc tttttcgata ccaaagttgg cctgtcaggc
     9601 ggccgccacg gcgcgtttcg tcatcacctt gacgagattg gcgcgatagg cggcgcttgc
     9661 atgaatatcg gaaagcaggt cggtacccgc tcttggactc ctgttgatag atccagtaat
     9721 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
     9781 tggtgagaat ccaggcaatt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     9841 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     9901 tgagccatat tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc aacatggatg
     9961 cggatctgta tggctataaa tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt
    10021 atcgtctgta tggcaaaccg gatgcgccgg aactgtttct gaaacatggc aaaggcagcg
    10081 tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc
    10141 tgccgaccat taaacatttt attcgcaccc cggatgatgc gtggctgctg accaccgcga
    10201 ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc ggatagcggc gaaaacattg
    10261 tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt
    10321 ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac aacggcctgg
    10381 tggatgcgag cgattttgat gatgaacgta acggctggcc ggtggaacag gtgtggaaag
    10441 aaatgcataa actgctgccg tttagcccgg atagcgtggt gacccacggc gattttagcc
    10501 tggataacct gattttcgat gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg
    10561 gcattgcgga tcgttatcag gatctggcca ttctgtggaa ctgcctgggc gaatttagcc
    10621 cgagcctgca aaaacgtctg tttcagaaat atggcattga taatccggat atgaacaaac
    10681 tgcaatttca tctgatgctg gatgaatttt tctaataatt aattgaacta cttttccgct
    10741 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
    10801 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
    10861 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
    10921 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc
    10981 ggccctaccg gcgcggcagc gttacccgtg tcggcggctc caacggctcg ccatcgtcca
    11041 gaaaacacgg ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc cattcctccg
    11101 tttcggtcaa ggctggcagg tctggttcca tgcccggaat gccgggctgg ctgggcggct
    11161 cctcgccggg gccggtcggt agttgctgct cgcccggata cagggtcggg atgcggcgca
    11221 ggtcgccatg ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac
    11281 tgaacaccga caggcgcaac tggtcgcggg gctggcccca cgccacgcgg tcattgacca
    11341 cgtaggccga cacggtgccg gggccgttga gcttcacgac ggagatccag cgctcggcca
    11401 ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc ttggaaagag
    11461 tcttttggct gaccaccacg gcgttctggt ggcccatctg cgcgacgagg tgatgcagca
    11521 gcattgccgc cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt
    11581 ccgtttggac ccagtgaccg ggcttgttct tggcttgaat gccgatttct ctggactgcg
    11641 tggccatgct tatctccatg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca
    11701 gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta
    11761 cagattttct ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg
    11821 tacccccctt ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt
    11881 ctttggtgcc caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc
    11941 cctccggcaa aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg
    12001 cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca
    12061 ggcgggcggg cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg
    12121 cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt cctcttggtg
    12181 tccaaccggc aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta
    12241 aaaaaattga tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga
    12301 aggctgggta gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc
    12361 atcagcttgt ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct
    12421 cgcggccatc gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
    12481 agcccggaga gcaagcccgt agggggttac tctagggcgg cggatttgtc ctactcagga
    12541 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
    12601 gttttatttg atgcctgccg aattcggatc cggagtaaca ggagtccaag agcgggtacg
    12661 tacccatcgg catttctttt gcgttt
//
