LOCUS       sequence-339564-        4589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30 encoding Wzz mutant  i161.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Papadopoulos M, Morona R
  TITLE     Mutagenesis and chemical cross-linking suggest that Wzz dimer 
            stability and oligomerization affect lipopolysaccharide O-antigen 
            modal chain length control.
  JOURNAL   J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. 
            Epub 2010 May 7.
  PUBMED    20453100
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01134-09"; journalName: "J Bacteriol"; date: "2010-07"; 
            volume: "192"; issue: "13"; pages: "3385-93"
COMMENT     Sequence Label: pMPRMA44
FEATURES             Location/Qualifiers
     source          1..4589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..54
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    98..114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             133..144
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             163..180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1404..1498
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1542..2201
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1608..1627)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2266..2352
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2399..2421)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2507..2647
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2562..2581
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2662..2679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2833..3421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2913..2932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3592..4383,4384..4452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4215..4234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4453..4557)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcacct cgagaaatca taaaaaattt atttgctttg tgagcggata acaatttcac
      121 acagaattca ttaaagagga gaaattaact atgagaggat cgcatcacca tcaccatcac
      181 ggatccgcat gcgagctcag agtagaaaat aataatgttt ctgggcaaaa ccatgacccg
      241 gaacagattg atttgattga tttactagtg cagttgtggc gtggcaagat gacaattatc
      301 atttccgtca ttgtggctat tgccctggct attggttatt tggcagtagc gaaggagaaa
      361 tggacgtcaa cagcaattat cactcagccc gacgtggggc aaattgctgg ctataacaat
      421 gccatgaatg ttatctatgg tcaggctgca ccgaaagtat cggatttgca ggagacgtta
      481 attggtcgct tcagttctgc cttctctgca ttagcagaaa cgctggataa tcaggaagag
      541 ccagaaaaac ttaccatcga accttctgtt aagaaccagc aattaccatt gactgtttct
      601 tatgttgggc aaactgcaga gggcgcacaa atgaagttgg cccaatacat tcagcaagtt
      661 gatgataaag tgaatcaaga tgcggccgca caagagctag aaaaggatct caaggacaac
      721 attgctctgg gacggaaaaa cttgcaggac tctttaagaa cccaggaagt ggtcgcgcag
      781 gagcagaaag atctgcgtat ccgtcagatt caggaagcgt tgcagtatgc gaatcaggcg
      841 caggtgacaa agccacaggt tcagcagact gaagatgtga cgcaagatac gttgttcctt
      901 ctagggagcg aagcgctgga gtcgatgatt aagcatgaag cgactcgtcc gttggtgttc
      961 tcaccaaact actatcagac acgtcaaaac ctgttggata ttgaaaaatt aaagtttgat
     1021 gatcttgata ttcatgctta ccgctatgtg atgaaaccga cgttacctat tcgtcgcgat
     1081 agtccgaaaa aggcaatcac cttgattctg gcagtgcttc tgggcggcat ggttggcgcg
     1141 gggattgtgt tggggcgtaa cgctctgcgt aattacaacg cgaagtaata ttattgtgca
     1201 tttaagagaa acgggcaggg tggtgacacc atgcccgttt tttttgccgg atgcgatgct
     1261 ggcgcatctt atccggccta cgtgtgttga gataatgtgt aggcacgata agtttgcgca
     1321 tcgggcaatg gctccgggtg tgacaacaac atcacacctg ctccccgggt cgacctgcag
     1381 ccaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact
     1441 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     1501 aagctagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     1561 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     1621 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     1681 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     1741 tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     1801 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1861 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1921 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     1981 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     2041 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     2101 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     2161 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     2221 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
     2281 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     2341 agtaggacaa atccgccctc tagagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     2401 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     2461 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     2521 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     2581 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     2641 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3241 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3661 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4561 atttccccga aaagtgccac ctgacgtct
//
