LOCUS       sequence-339567-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 P.2/B.1.1.248 Spike Protein lacking 18 C-terminal
            amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 P.2/B.1.1.248
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3929..4517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3982..4002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4400..4411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4570..4691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4581..4600)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4635..4654
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4806..4852
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4882..5018
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5063..5134
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5179..5375
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5226..5361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5389..6183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5443..5462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6053..6072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6194..6418
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6614..7202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6694..6713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7948..8016,8017..8808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8166..8185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8821..8957
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9109..9180
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9185..9231)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9249..9297
                     /note="synthetic polyadenylation signal"
     enhancer        9378..9681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9683..9885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9836..9856
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          join(10019..10462,1..32)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
       61 tggcccatca ctttggcaaa ggcggccgcc atgtttgtgt tcctcgttct cctgcccctc
      121 gtttcttccc aatgcgtgaa cctcacaact agaacgcagc tcccacccgc ttacacgaac
      181 tcttttactc gtggagtata ctatcccgat aaagtcttta ggtccagtgt ccttcattct
      241 actcaagacc ttttcctgcc attcttctca aatgtcacct ggttccatgc tatccacgtt
      301 tctggcacca acggaactaa acgatttgat aatcccgtct tgccatttaa cgatggcgta
      361 tactttgcct caacggagaa gtccaacatt atccggggat ggatatttgg caccaccctg
      421 gactccaaga cacagtctct cctcattgtg aacaacgcca ccaatgtcgt catcaaagtc
      481 tgtgaatttc aattttgtaa cgatcccttt ctcggggttt attatcataa gaataacaag
      541 tcttggatgg agtcagagtt ccgcgtttac agttccgcta acaactgtac ttttgagtac
      601 gtttcccaac catttctcat ggatcttgaa gggaagcaag gcaacttcaa gaatctccgc
      661 gaatttgtgt ttaagaatat agacggctac ttcaaaattt atagcaagca taccccgata
      721 aatctggttc gggatctccc tcaaggcttc tccgcgttgg aacccctcgt ggacttgccg
      781 attggcatca acatcacccg gttccaaacc cttcttgccc ttcatcgaag ctacctcacg
      841 cctggcgaca gtagtagtgg ttggacagcg ggcgcagcag cctactatgt gggatatttg
      901 caaccacgca cctttcttct caaatataac gaaaacggaa caattaccga tgccgtagac
      961 tgtgccctcg atcccctgtc cgaaactaaa tgcacactca agtccttcac agttgagaaa
     1021 ggcatatacc aaaccagtaa cttccgcgtc caacccaccg aatccattgt tcgattccct
     1081 aacatcacca atttgtgtcc tttcggtgaa gtatttaatg ccactcgatt tgcatcagtt
     1141 tatgcctgga atcgcaagcg aatatcaaat tgtgttgcag attatagtgt cctgtataat
     1201 agcgcatcat tcagcacttt taagtgttat ggggtcagcc ctacgaaatt gaatgacttg
     1261 tgttttacca acgtttacgc agattctttc gtcatacgcg gcgacgaagt ccgacagatt
     1321 gcaccggggc aaaccggaaa gatagcagac tataattata agttgccgga tgacttcacc
     1381 ggctgcgtta tagcatggaa ctccaataat ctcgattcaa aagtcggcgg caactataat
     1441 tatctttacc gcctgtttcg caagtcaaat ctgaagccct ttgaacgtga catctccacc
     1501 gaaatttatc aagctggcag caccccatgt aatggagtga aagggtttaa ctgttatttt
     1561 ccccttcaat catacggatt tcaaccaaca aatggggtgg gctatcaacc atatcgggtg
     1621 gtagtcttga gctttgagct gctccacgca ccggctacag tttgcggacc gaagaaaagc
     1681 actaacctgg tgaagaataa atgtgtgaac ttcaacttta atgggctcac cggaacaggg
     1741 gtgctgacag aatcaaacaa gaaatttctc ccttttcaac aatttggtag agacattgcc
     1801 gataccacag atgccgtccg cgatccgcaa acactggaaa tcctcgatat aacaccctgt
     1861 agcttcggcg gggtcagcgt gataactccc ggaacgaaca catccaacca agtggctgtc
     1921 ctgtatcaag gtgtcaactg taccgaagtc cctgtggcga tacacgccga ccaactgaca
     1981 cccacctgga gagtctactc aacaggttcc aatgtctttc aaactagagc gggttgtctc
     2041 attggcgctg agcatgtcaa taattcatac gaatgtgata ttcctattgg ggcaggaatc
     2101 tgtgcgtctt atcaaactca aacgaactca ccacggcgcg ctcgctcagt agccagccag
     2161 tccataatcg cctataccat gagtctcggg gctgagaact ccgtggctta ttcaaacaat
     2221 tccatcgcaa tcccaactaa ctttaccatt agcgttacca cggaaatctt gcccgtcagc
     2281 atgactaaga cgagcgtaga ctgtacgatg tatatctgtg gggattccac cgagtgtagc
     2341 aacctcttgc ttcaatacgg gtctttctgt actcaactca accgagcatt gaccggaatt
     2401 gcggtcgaac aagacaagaa tacccaagag gtgttcgcac aagtaaagca aatatacaaa
     2461 acgccgccaa tcaaagattt tggcgggttt aattttagcc aaatactgcc agacccatct
     2521 aagccttcaa aacgcagctt tatcgaggat ctgctgttca acaaagttac gttggctgac
     2581 gcgggtttca ttaagcagta tggggattgt ttgggagaca ttgcggcccg cgaccttatc
     2641 tgtgctcaaa aattcaatgg gttgacagtt ctgccccctc tgctcactga tgagatgatt
     2701 gcacagtata catcagcatt gttggcagga accattacat ccggctggac ttttggcgcg
     2761 ggtgcggccc ttcaaatacc tttcgctatg caaatggctt acagattcaa tgggattggt
     2821 gtcacgcaaa atgtattgta tgaaaatcag aagctgatcg ctaatcaatt taacagcgcg
     2881 attggcaaga tccaagattc actgtcaagc acagcgtccg ccctcggcaa actccaagat
     2941 gttgtgaatc aaaacgccca ggccctgaat accctcgtga agcaactgag ttctaacttc
     3001 ggcgcaatct ccagcgtcct caacgacata ttgtccagac ttgataaggt ggaagccgag
     3061 gtgcaaattg atcggctcat cacaggcagg ctccagtcac tccaaactta tgtgacacaa
     3121 cagttgatcc gggctgctga gatacgagca agcgccaacc tggccgccac taaaatgtca
     3181 gagtgcgtgc tcgggcagtc caaacgggtt gacttttgtg gcaagggcta tcacttgatg
     3241 tccttccccc aatccgcccc acatggagtg gtctttctcc acgtcactta tgtccctgct
     3301 caagagaaga actttacgac agctccagca atctgtcacg atggcaaagc gcactttccc
     3361 agggaaggcg ttttcgtcag taatggcacg cattggtttg tgacccaaag gaatttctat
     3421 gaaccccaaa tcataaccac tgacaataca tttgtctcag gcaactgtga tgtggtcatt
     3481 gggatcgtga acaacacggt atatgatccc ctgcaacccg agttggatag cttcaaagag
     3541 gaattggata aatactttaa gaatcatact tcacccgatg ttgatctcgg ggacatatca
     3601 ggaataaatg caagtttcgt caacattcag aaagagattg atcgccttaa tgaggtcgcc
     3661 aagaacttga atgaatcttt gatcgacttg caagaactgg gcaaatacga acaatatatt
     3721 aaatggccct ggtacatttg gttgggcttt atcgccggac ttatagctat cgttatggtc
     3781 acaattatgt tgtgctgtat gactagctgt tgttcctgct tgaaaggatg ttgcagttgc
     3841 ggatcttgtt gtaaataaag gatcctctag aagttgtctc ctcctgcact gactgactga
     3901 tacaatcgat ttctggatcc gcaggcctaa tcaacctctg gattacaaaa tttgtgaaag
     3961 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4021 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4081 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4141 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4201 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4261 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4321 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4381 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4441 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4501 ttgggccgcc tccccgcctg tctagcttga ctgactgaga tacagcgtac cttcagctca
     4561 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     4621 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     4681 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     4741 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tattggccca tctctatcgg
     4801 tatcgtagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgtgcccctc
     4861 gggccggatt gctatctacc ggcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc
     4921 gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac
     4981 ttccatacgt gtcctcctta ccagaaattt atccttaagg tcgtcagcta tcctgcaggc
     5041 gatctctcga tttcgatcaa gacattcctt taatggtctt ttctggacac cactaggggt
     5101 cagaagtagt tcatcaaact ttcttccctc cctaatctca ttggttacct tgggctatcg
     5161 aaacttaatt aagcgatctg catctcaatt agtcagcaac catagtcccg cccctaactc
     5221 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat cgctgactaa
     5281 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5341 gaggaggctt ttttggaggc ctaggctttt gcaaaggagg tagccaacat gattgaacaa
     5401 gatggattgc acgcaggttc tcccgccgct tgggtggaga ggctattcgg ctatgactgg
     5461 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5521 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca ggacgaggca
     5581 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5641 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5701 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5761 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5821 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5881 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc
     5941 gtcgtgaccc acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6001 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6061 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6121 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6181 tagtatgtaa gccctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6241 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6301 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6361 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggtt
     6421 aattaaccag tcaagtcagc tacttggcga gatcgacttg tctgggtttc gactacgctc
     6481 agaattgcgt cagtcaagtt cgatctggtc cttgctattg cacccgttct ccgattacga
     6541 gtttcattta aatcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6601 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6661 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6721 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6781 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6841 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6901 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6961 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7021 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7081 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7141 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7201 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7261 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7321 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7381 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcat
     7441 ttaaatttcc gaactctcca aggccctcgt cggaaaatct tcaaaccttt cgtccgatcc
     7501 atcttgcagg ctacctctcg aacgaactat cgcaagtctc ttggccggcc ttgcgccttg
     7561 gctattgctt ggcagcgcct atcgccaggt attactccaa tcccgaatat ccgagatcgg
     7621 gatcacccga gagaagttca acctacatcc tcaatcccga tctatccgag atccgaggaa
     7681 tatcgaaatc ggggcgcgcc tggtgtaccg agaacgatcc tctcagtgcg agtctcgacg
     7741 atccatatcg ttgcttggca gtcagccagt cggaatccag cttgggaccc aggaagtcca
     7801 atcgtcagat attgtactca agcctggtca cggcagcgta ccgatctgtt taaacctaga
     7861 tattgatagt ctgatcggtc aacgtataat cgagtcctag cttttgcaaa catctatcaa
     7921 gagacaggat cagcaggagg ctttcgcatg agtattcaac atttccgtgt cgcccttatt
     7981 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8041 aaagatgctg aagatcagtt gggtgcgcga gtgggttaca tcgaactgga tctcaacagc
     8101 ggtaagatcc ttgagagttt tcgccccgaa gaacgctttc caatgatgag cacttttaaa
     8161 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8221 cgcatacact attctcagaa tgacttggtt gagtattcac cagtcacaga aaagcatctt
     8281 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8341 gcggccaact tacttctgac aacgattgga ggaccgaagg agctaaccgc ttttttgcac
     8401 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8461 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacctt gcgtaaacta
     8521 ttaactggcg aactacttac tctagcttcc cggcaacagt tgatagactg gatggaggcg
     8581 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8641 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8701 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8761 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaacc gattctaggt
     8821 gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc
     8881 cagattcagc aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac
     8941 cagaaattta tccttaagat cccgaatcgt ttaaactcga ctctggctct atcgaatctc
     9001 cgtcgtttcg agcttacgcg aacagccgtg gcgctcattt gctcgtcggg catcgaatct
     9061 cgtcagctat cgtcagctta cctttttggc agcgatcgcg gctcccgaca tcttggacca
     9121 ttagctccac aggtatcttc ttccctctag tggtcataac agcagcttca gctacctctc
     9181 aattcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct atcaatcgtt
     9241 gcgttacaca cacaaaaaac caacacacat ccatcttcga tggatagcga ttttattatc
     9301 taactgctga tcgagtgtag ccagatctag taatcaatta cggggtcatt agttcatagc
     9361 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     9421 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     9481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     9541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     9601 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     9661 ttagtcatcg ctattaccat gctgatgcgg ttttggcagt acatcaatgg gcgtggatag
     9721 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     9781 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     9841 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     9901 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     9961 tccagcctcc cctcgaagct tacatgtggt accgagctcg gatcctgaga acttcagggt
    10021 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
    10081 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
    10141 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
    10201 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
    10261 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
    10321 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
    10381 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
    10441 ccaagctagg cccttttgct aa
//
