LOCUS       sequence-339568-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal 
            amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.1.298
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          656..1131
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5022..5610
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5075..5095)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5493..5504)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5663..5784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5674..5693)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5728..5747
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      5899..5945
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      5975..6111
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6156..6227
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        6272..6468
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6319..6454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6482..7276
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6536..6555)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7146..7165
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7287..7511
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7707..8295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7787..7806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9041..9109,9110..9901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9259..9278)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      9914..10050
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    10202..10273
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10278..10324)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10342..10390
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatgct gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctcccct cgaagcttac atgtggtacc gagctcggat cctgagaact tcagggtgag
      661 tctatgggac ccttgatgtt ttctttcccc ttcttttcta tggttaagtt catgtcatag
      721 gaaggggaga agtaacaggg tacacatatt gaccaaatca gggtaatttt gcatttgtaa
      781 ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
      841 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
      901 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
      961 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     1021 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     1081 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc
     1141 aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag gcggccgcca tgtttgtgtt
     1201 cctcgttctc ctgcccctcg tttcttccca atgcgtgaac ctcacaacta gaacgcagct
     1261 cccacccgct tacacgaact cttttactcg tggagtatac tatcccgata aagtctttag
     1321 gtccagtgtc cttcattcta ctcaagacct tttcctgcca ttcttctcaa atgtcacctg
     1381 gttccatgct atctctggca ccaacggaac taaacgattt gataatcccg tcttgccatt
     1441 taacgatggc gtatactttg cctcaacgga gaagtccaac attatccggg gatggatatt
     1501 tggcaccacc ctggactcca agacacagtc tctcctcatt gtgaacaacg ccaccaatgt
     1561 cgtcatcaaa gtctgtgaat ttcaattttg taacgatccc tttctcgggg tttattatca
     1621 taagaataac aagtcttgga tggagtcaga gttccgcgtt tacagttccg ctaacaactg
     1681 tacttttgag tacgtttccc aaccatttct catggatctt gaagggaagc aaggcaactt
     1741 caagaatctc cgcgaatttg tgtttaagaa tatagacggc tacttcaaaa tttatagcaa
     1801 gcataccccg ataaatctgg ttcgggatct ccctcaaggc ttctccgcgt tggaacccct
     1861 cgtggacttg ccgattggca tcaacatcac ccggttccaa acccttcttg cccttcatcg
     1921 aagctacctc acgcctggcg acagtagtag tggttggaca gcgggcgcag cagcctacta
     1981 tgtgggatat ttgcaaccac gcacctttct tctcaaatat aacgaaaacg gaacaattac
     2041 cgatgccgta gactgtgccc tcgatcccct gtccgaaact aaatgcacac tcaagtcctt
     2101 cacagttgag aaaggcatat accaaaccag taacttccgc gtccaaccca ccgaatccat
     2161 tgttcgattc cctaacatca ccaatttgtg tcctttcggt gaagtattta atgccactcg
     2221 atttgcatca gtttatgcct ggaatcgcaa gcgaatatca aattgtgttg cagattatag
     2281 tgtcctgtat aatagcgcat cattcagcac ttttaagtgt tatggggtca gccctacgaa
     2341 attgaatgac ttgtgtttta ccaacgttta cgcagattct ttcgtcatac gcggcgacga
     2401 agtccgacag attgcaccgg ggcaaaccgg aaagatagca gactataatt ataagttgcc
     2461 ggatgacttc accggctgcg ttatagcatg gaactccaat aatctcgatt caaaagtcgg
     2521 cggcaactat aattatcttt tccgcctgtt tcgcaagtca aatctgaagc cctttgaacg
     2581 tgacatctcc accgaaattt atcaagctgg cagcacccca tgtaatggag tggaagggtt
     2641 taactgttat tttccccttc aatcatacgg atttcaacca acaaatgggg tgggctatca
     2701 accatatcgg gtggtagtct tgagctttga gctgctccac gcaccggcta cagtttgcgg
     2761 accgaagaaa agcactaacc tggtgaagaa taaatgtgtg aacttcaact ttaatgggct
     2821 caccggaaca ggggtgctga cagaatcaaa caagaaattt ctcccttttc aacaatttgg
     2881 tagagacatt gccgatacca cagatgccgt ccgcgatccg caaacactgg aaatcctcga
     2941 tataacaccc tgtagcttcg gcggggtcag cgtgataact cccggaacga acacatccaa
     3001 ccaagtggct gtcctgtatc aaggtgtcaa ctgtaccgaa gtccctgtgg cgatacacgc
     3061 cgaccaactg acacccacct ggagagtcta ctcaacaggt tccaatgtct ttcaaactag
     3121 agcgggttgt ctcattggcg ctgagcatgt caataattca tacgaatgtg atattcctat
     3181 tggggcagga atctgtgcgt cttatcaaac tcaaacgaac tcaccacggc gcgctcgctc
     3241 agtagccagc cagtccgtaa tcgcctatac catgagtctc ggggctgaga actccgtggc
     3301 ttattcaaac aattccatcg caatcccaac taactttacc attagcgtta ccacggaaat
     3361 cttgcccgtc agcatgacta agacgagcgt agactgtacg atgtatatct gtggggattc
     3421 caccgagtgt agcaacctct tgcttcaata cgggtctttc tgtactcaac tcaaccgagc
     3481 attgaccgga attgcggtcg aacaagacaa gaatacccaa gaggtgttcg cacaagtaaa
     3541 gcaaatatac aaaacgccgc caatcaaaga ttttggcggg tttaatttta gccaaatact
     3601 gccagaccca tctaagcctt caaaacgcag ctttatcgag gatctgctgt tcaacaaagt
     3661 tacgttggct gacgcgggtt tcattaagca gtatggggat tgtttgggag acattgcggc
     3721 ccgcgacctt atctgtgctc aaaaattcaa tgggttgaca gttctgcccc ctctgctcac
     3781 tgatgagatg attgcacagt atacatcagc attgttggca ggaaccatta catccggctg
     3841 gacttttggc gcgggtgcgg cccttcaaat acctttcgct atgcaaatgg cttacagatt
     3901 caatgggatt ggtgtcacgc aaaatgtatt gtatgaaaat cagaagctga tcgctaatca
     3961 atttaacagc gcgattggca agatccaaga ttcactgtca agcacagcgt ccgccctcgg
     4021 caaactccaa gatgttgtga atcaaaacgc ccaggccctg aataccctcg tgaagcaact
     4081 gagttctaac ttcggcgcaa tctccagcgt cctcaacgac atattgtcca gacttgataa
     4141 ggtggaagcc gaggtgcaaa ttgatcggct catcacaggc aggctccagt cactccaaac
     4201 ttatgtgaca caacagttga tccgggctgc tgagatacga gcaagcgcca acctggccgc
     4261 cactaaaatg tcagagtgcg tgctcgggca gtccaaacgg gttgactttt gtggcaaggg
     4321 ctatcacttg atgtccttcc cccaatccgc cccacatgga gtggtctttc tccacgtcac
     4381 ttatgtccct gctcaagaga agaactttac gacagctcca gcaatctgtc acgatggcaa
     4441 agcgcacttt cccagggaag gcgttttcgt cagtaatggc acgcattggt ttgtgaccca
     4501 aaggaatttc tatgaacccc aaatcataac cactgacaat acatttgtct caggcaactg
     4561 tgatgtggtc attgggatcg tgaacaacac ggtatatgat cccctgcaac ccgagttgga
     4621 tagcttcaaa gaggaattgg ataaatactt taagaatcat acttcacccg atgttgatct
     4681 cggggacata tcaggaataa atgcaagtgt cgtcaacatt cagaaagaga ttgatcgcct
     4741 taatgaggtc gccaagaact tgaatgaatc tttgatcgac ttgcaagaac tgggcaaata
     4801 cgaacaatat attaaatggc cctggtacat ttggttgggc tttatcgccg gacttatagc
     4861 tatcgttatc gtcacaatta tgttgtgctg tatgactagc tgttgttcct gcttgaaagg
     4921 atgttgcagt tgcggatctt gttgtaaata aaggatcctc tagaagttgt ctcctcctgc
     4981 actgactgac tgatacaatc gatttctgga tccgcaggcc taatcaacct ctggattaca
     5041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5581 gtcggatctc cctttgggcc gcctccccgc ctgtctagct tgactgactg agatacagcg
     5641 taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac cacaactaga
     5701 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     5761 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     5821 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtattggc
     5881 ccatctctat cggtatcgta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     5941 ttttgtgccc ctcgggccgg attgctatct accggcattg gcgcagaaaa aaatgcctga
     6001 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc
     6061 ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta aggtcgtcag
     6121 ctatcctgca ggcgatctct cgatttcgat caagacattc ctttaatggt cttttctgga
     6181 caccactagg ggtcagaagt agttcatcaa actttcttcc ctccctaatc tcattggtta
     6241 ccttgggcta tcgaaactta attaagcgat ctgcatctca attagtcagc aaccatagtc
     6301 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6361 catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6421 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg aggtagccaa
     6481 catgattgaa caagatggat tgcacgcagg ttctcccgcc gcttgggtgg agaggctatt
     6541 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6601 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6661 ccaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6721 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6781 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6841 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6901 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6961 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc ggatgcccga
     7021 cggcgaggat ctcgtcgtga cccacggcga tgcctgcttg ccgaatatca tggtggaaaa
     7081 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     7141 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     7201 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     7261 tgacgagttc ttctagtatg taagccctgt gccttctagt tgccagccat ctgttgtttg
     7321 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     7381 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     7441 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     7501 gggctctatg gttaattaac cagtcaagtc agctacttgg cgagatcgac ttgtctgggt
     7561 ttcgactacg ctcagaattg cgtcagtcaa gttcgatctg gtccttgcta ttgcacccgt
     7621 tctccgatta cgagtttcat ttaaatcatg tgagcaaaag gccagcaaaa ggccaggaac
     7681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8161 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8221 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8281 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8341 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8401 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8461 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8521 tccatagttg catttaaatt tccgaactct ccaaggccct cgtcggaaaa tcttcaaacc
     8581 tttcgtccga tccatcttgc aggctacctc tcgaacgaac tatcgcaagt ctcttggccg
     8641 gccttgcgcc ttggctattg cttggcagcg cctatcgcca ggtattactc caatcccgaa
     8701 tatccgagat cgggatcacc cgagagaagt tcaacctaca tcctcaatcc cgatctatcc
     8761 gagatccgag gaatatcgaa atcggggcgc gcctggtgta ccgagaacga tcctctcagt
     8821 gcgagtctcg acgatccata tcgttgcttg gcagtcagcc agtcggaatc cagcttggga
     8881 cccaggaagt ccaatcgtca gatattgtac tcaagcctgg tcacggcagc gtaccgatct
     8941 gtttaaacct agatattgat agtctgatcg gtcaacgtat aatcgagtcc tagcttttgc
     9001 aaacatctat caagagacag gatcagcagg aggctttcgc atgagtattc aacatttccg
     9061 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9121 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcg cgagtgggtt acatcgaact
     9181 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgct ttccaatgat
     9241 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9301 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt caccagtcac
     9361 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9421 gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga aggagctaac
     9481 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9541 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9601 cttgcgtaaa ctattaactg gcgaactact tactctagct tcccggcaac agttgataga
     9661 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9721 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9781 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9841 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9901 accgattcta ggtgcattgg cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata
     9961 ctcccacata tgccagattc agcaacggat acggcttccc caacttgccc acttccatac
    10021 gtgtcctcct taccagaaat ttatccttaa gatcccgaat cgtttaaact cgactctggc
    10081 tctatcgaat ctccgtcgtt tcgagcttac gcgaacagcc gtggcgctca tttgctcgtc
    10141 gggcatcgaa tctcgtcagc tatcgtcagc ttaccttttt ggcagcgatc gcggctcccg
    10201 acatcttgga ccattagctc cacaggtatc ttcttccctc tagtggtcat aacagcagct
    10261 tcagctacct ctcaattcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
    10321 gctatcaatc gttgcgttac acacacaaaa aaccaacaca catccatctt cgatggatag
    10381 cgattttatt atctaactgc tgatcgagtg tagccagatc tagtaatcaa ttacggggtc
    10441 attagttcat agccca
//
