LOCUS       sequence-33957-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pcdna6.2- myc ULK1 wt
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             133..162
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gactctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagttaagc tgagcatcaa caagtttgta caaaaaagca
      121 ggcttcacca tggaacaaaa gctaatctcc gaggaagact tgggaggaat agagccgggc
      181 cgaggcggcg tcgagaccgt gggcaagttc gagttctctc gcaaggacct gattggacac
      241 ggcgccttcg cggtggtctt caagggtcga caccgcgaga agcacgacct ggaggtggcc
      301 gtcaaatgca ttaacaagaa gaaccttgcc aagtcccaaa cactgctggg aaaggaaatc
      361 aaaatcctga aggaactaaa gcacgaaaac atcgtggcgc tgtatgactt ccaggaaatg
      421 gctaattctg tctacctggt catggagtat tgtaatggtg gagacctggc tgactacctg
      481 cacactatgc gcacactgag tgaagacact gtcaggcttt tcctacagca gatcgctggc
      541 gccatgcggc tgctgcacag caagggcatc atccaccggg acctgaagcc ccaaaacatc
      601 ctgctgtcca accctggggg ccgccgggcc aaccccagca acatccgagt caagattgct
      661 gactttggat tcgctcggta cctccagagc aacatgatgg cggccacact ctgtggttct
      721 cctatgtaca tggctcctga ggtca
//
