LOCUS       sequence-339572-        3033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An rrnB P1-based GFP ATP reporter  in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Deng Y, Beahm DR, Ionov S, Sarpeshkar R
  TITLE     Measuring and modeling energy and power consumption in living 
            microbial cells with a synthetic ATP reporter.
  JOURNAL   BMC Biol. 2021 May 17;19(1):101. doi: 10.1186/s12915-021-01023-2.
  PUBMED    34001118
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2021-05-17"; volume: "19(1):101. doi"; pages: " 
            10.1186/s12915-021-01023-2"
COMMENT     Sequence Label: HC-M
FEATURES             Location/Qualifiers
     source          1..3033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1058..1144
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1308..1896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1388..1407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1984..2078)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2108..2899,2900..2968))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2731..2750
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2969..3033,1..40))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
       61 cacctgacgt cgccaggagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa
      121 acgctcgaaa aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcagaaaatt
      181 attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac caaacattca
      241 ccgatatccg aacggaggta gttatgtcga aaggtgagga gttattcaca ggggtcgtgc
      301 ccattttagt ggaacttgac ggagatgtca acggtcacaa gtttagtgtg tctggcgagg
      361 gggaggggga tgccacgtat ggcaagttga ccttgaagtt tatctgtact accggaaaat
      421 tacccgtgcc gtggccgaca ttggtcacaa cttttgctta cggtcttcag tgcttcgctc
      481 gctaccccga tcatatgaag cagcatgatt ttttcaaaag cgcgatgccc gaggggtatg
      541 ttcaagaacg taccattttc ttcaaggacg atggcaacta caagactcgt gcagaggtca
      601 aatttgaggg tgacactctt gtaaatcgca ttgagttaaa gggtatcgat ttcaaagaag
      661 acggcaatat tcttgggcac aaattagaat acaactacaa cagccataac gtgtatatta
      721 tggctgacaa acaaaagaat ggaattaagg ttaattttaa gatccgccat aatattgagg
      781 acggatcagt tcagctggcg gatcactatc aacagaatac accgatcggt gacggccctg
      841 tgctgcttcc agacaaccac tatttatcca ctcagtccaa gttatctaaa gaccccaatg
      901 aaaaacgcga tcatatggtt ttgttagagt tcgtaacggc tgcaggaatc actcatggta
      961 tggacgagtt gcataaaagg cctgcagcaa acgacgaaaa ctacgcttta gcagcttaat
     1021 aacccggggg atcccatggt acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc
     1081 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     1141 aaatccgccg ccctagacct aggcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1201 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1261 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1321 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1741 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1921 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gactagtgct
     1981 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     2041 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt
     2101 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2161 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2221 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2281 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2341 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2401 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2461 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2521 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2581 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2641 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2701 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2761 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2821 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2881 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2941 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3001 atcagggtta ttgtctcatg agcggataca tat
//
