LOCUS       sequence-339574-        4845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A3_STOP
FEATURES             Location/Qualifiers
     source          1..4845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2402..2501)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2518..2537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2519..2537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2542..2558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2542..2558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2660..2679)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2671..3480
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2738..2757)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3627..4215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4116..4135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4369..4386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4545..4572)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4664..4750)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4799..4821
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4813..4830
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4814..4830
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgat cgagcccttc
      121 ggcaaccagt acatcgtggc ccggcccgtg tactctacca acgccttcga ggagaatcac
      181 aagaagaccg gcagacacca caagacattt ctggatcacc tgaaggtgtg ctgtagctgc
      241 tcccctcaga aggccaagag gatcgtgctg tccctgttcc ctatcgcctc ttggctgcca
      301 gcctaccgcc tgaaggagtg gctgctgagc gatatcgtgt ctggcatcag caccggaatc
      361 gtggccgtgc tgcagggact ggcctttgcc ctgctggtgg acatcccccc cgtgtacggc
      421 ctgtatgcct ccttctttcc agccatcatc tatctgttct ttggcacatc ccggcacatc
      481 tctgtgggcc ctttcccaat cctgtctatg atggtgggac tggccgtgag cggagccgtg
      541 tccaaggccg tgccagacag aaatgccacc acactgggcc tgcccaacaa tagcaacaat
      601 agctccctgc tggacgatga gagggtgcgc gtggcagcag cagcctctgt gaccgtgctg
      661 agcggcatca tccagctggc cttcggcatc ctgcggatcg gctttgtggt catctacctg
      721 tctgagagcc tgatctccgg ctttaccaca gcagcagcag tgcacgtgct ggtgtctcag
      781 ctgaagttca tctttcagct gaccgtgcca agccacacag atcccgtgtc catcttcaag
      841 gtgctgtatt ccgtgttttc tcagatcgag aagaccaaca tcgccgacct ggtgacagcc
      901 ctgatcgtgc tgctggtggt gagcatcgtg aaggagatca atcagaggtt caaggacaag
      961 ctgcccgtgc ctatcccaat cgagtttatc atgaccgtga tcgcagcagg cgtgtcctac
     1021 ggctgcgatt tcaagaaccg ctttaaggtg gccgtggtgg gcgacatgaa tcctggcttc
     1081 cagccaccca tcacaccaga tgtggagacc ttccagaaca cagtgggcga ctgttttgga
     1141 atcgcaatgg tggccttcgc cgtggccttt agcgtggcct ccgtgtattc tctgaagtac
     1201 gattatcctc tggacggcaa ccaggagctg atcgccctgg gcctgggcaa tatcgtgtgc
     1261 ggcgtgttcc ggggatttgc aggatctacc gccctgtcca gatctgccgt gcaggagagc
     1321 accggaggca agacacagat cgcaggactg atcggcgcca tcatcgtgct gatcgtggtg
     1381 ctggcaatcg gcttcctgct ggcacccctg cagaagtccg tgctggccgc cctggccctg
     1441 ggaaacctga agggcatgct gatgcagttt gccgagatcg gccggctgtg gagaaaggat
     1501 aagtacgact gtctgatctg gatcatgacc ttcatcttta caatcgtgct gggactggga
     1561 ctgggactgg cagccagcgt ggcctttcag ctgctgacca tcgtgttccg gacacagttt
     1621 cctaagtgct ccaccctggc caatatcggc agaacaaaca tctacaagaa taagaaggat
     1681 tactacgaca tgtacgagcc agagggcgtg aagatcttca ggtgtccctc tcctatctac
     1741 tttgccaaca tcggcttctt tcggagaaag ctgatcgatg ccgtgggctt cagcccactg
     1801 cgcatcctgc ggaagagaaa taaggccctg aggaagatcc gcaagctgca gaagcaggga
     1861 ctgctgcagg tgaccccaaa gggctttatc tgtaccgtgg acacaatcaa ggattccgac
     1921 gaggagctgg ataacaatca gatcgaggtg ctggaccagc caatcaacac cacagatctg
     1981 cccttccaca tcgactggaa cgacgatctg cccctgaata tcgaggtgcc taagatcagc
     2041 ctgcactccc tgatcctgga tttctctgcc gtgagctttc tggacgtgtc tagcgtgagg
     2101 ggcctgaaga gcatcctgca ggagttcatc cgcatcaagg tggacgtgta catcgtgggc
     2161 accgacgatg actttatcga gaagctgaat agatatgagt tctttgacgg cgaggtgaag
     2221 tcctctatct tctttctgac catccacgat gccgtgctgc acatcctgat gaagaaggac
     2281 tacagcacat ccaagttcaa cccctcccag gagaaggatg gcaagatcga ctttaccatc
     2341 aacacaaatg gcggcctgag gaatcgcgtg tatgaggtgc ctgtggagac aaagttctaa
     2401 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2461 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2521 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2581 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2641 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2701 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2761 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2821 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2881 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2941 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     3001 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3061 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3121 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3181 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3241 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     3301 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3361 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3421 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3481 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3541 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4261 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4321 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4381 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4441 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4501 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4561 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4621 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4681 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4741 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4801 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
