LOCUS       sequence-339578-       10668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, C1 Cloning Vector, Excitation:434, 
            Emission:474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10668)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-MyosinIIB-C-18
FEATURES             Location/Qualifiers
     source          1..10668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7396..7517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7433..7452)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7487..7506
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7524..7979)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7661..7682)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7873..7892
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8006..8110
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8112..8469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8112..8303
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8135..8155)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8320..8455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8504..9298
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8504..9298
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8558..8577)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9168..9187
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9486..9505)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9530..9577
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9906..10494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10395..10414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac atcagcgaca acgtctatat caccgccgac
     1021 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggaggtag cgcatccagt gggggtgcgt caggcagtgg cagcggatct
     1321 ggcgcgcaga gaactggact cgaggatcca gagaggtatc tctttgtgga cagggctgtc
     1381 atctacaacc ctgccactca agctgattgg acagctaaaa agctagtgtg gattccatca
     1441 gaacgccatg gttttgaggc agctagtatc aaagaagaac ggggagatga agttatggtg
     1501 gagttggcag agaatggaaa gaaagcaatg gtcaacaaag atgatattca gaagatgaac
     1561 ccacctaagt tttccaaggt ggaggatatg gcagaattga catgcttgaa tgaagcttcc
     1621 gttttacata atctgaagga tcgctactat tcaggactaa tctatactta ttctggactc
     1681 ttctgtgtag ttataaaccc ttacaagaat cttccaattt actctgagaa tattattgaa
     1741 atgtacagag ggaagaagcg tcatgagatg cctccacaca tctatgctat atctgaatct
     1801 gcttacagat gcatgcttca agatcgtgag gaccagtcaa ttctttgcac gggtgagtca
     1861 ggtgctggga agacagaaaa tacaaagaaa gttattcagt accttgccca tgttgcttct
     1921 tcacataaag gaagaaagga ccataatatt cctggggaac ttgaacggca gcttttgcaa
     1981 gcaaatccaa ttctggaatc atttggaaat gcgaagactg tgaaaaatga taactcatct
     2041 cgttttggca aatttattcg gatcaacttt gatgtaactg gctatatcgt tggggccaac
     2101 attgaaacat accttctgga aaagtctcgt gctgttcgtc aagcaaaaga tgaacgtact
     2161 tttcatatct tttaccagtt gttatctgga gcaggagaac acctaaagtc tgatttgctt
     2221 cttgaaggat ttaataacta caggtttctc tccaatggct atattcctat tccgggacag
     2281 caagacaaag ataatttcca ggagaccatg gaagcaatgc acataatggg cttctcccat
     2341 gaagagattc tgtcaatgct taaagtagta tcttcagtgc tacagtttgg aaatatttct
     2401 ttcaaaaagg agagaaatac tgatcaagct tccatgccag aaaatacagt tgcgcagaag
     2461 ctctgccatc ttcttgggat gaatgtgatg gagtttactc gggccatcct gactccccgg
     2521 atcaaggtcg gccgagacta tgtgcaaaaa gcccagacca aagaacaggc agattttgca
     2581 gtagaagcat tggcaaaagc tacctatgag cggctctttc gctggctcgt tcatcgcatc
     2641 aataaagctc tggataggac caaacgtcag ggagcatctt tcattggaat cctggatatt
     2701 gctggatttg aaatttttga gctgaactcc tttgaacaac tttgcatcaa ctacaccaat
     2761 gagaagctgc agcagctgtt caaccacacc atgtttatcc tagaacaaga ggaataccag
     2821 cgcgaaggca tcgagtggaa cttcatcgat ttcgggctgg atctgcagcc atgcatcgac
     2881 ctaatagaga gacctgcgaa ccctcctggt gtactggccc ttttggatga agaatgctgg
     2941 ttccctaaag ccacagataa aacctttgtt gaaaaactgg ttcaagagca aggttcccac
     3001 tccaagtttc agaaacctcg acaattaaaa gacaaagctg atttttgcat tatacattat
     3061 gcagggaagg tggactataa ggcagatgag tggctgatga agaatatgga ccccctgaat
     3121 gacaacgtgg ccaccctttt gcaccagtca tcagacagat ttgtggcaga gctttggaaa
     3181 gatgtggacc gtatcgtggg tctggatcaa gtcactggta tgactgagac agcttttggc
     3241 tccgcatata aaaccaagaa gggcatgttt cgtaccgttg ggcaactcta caaagaatct
     3301 ctcaccaagc tgatggcaac tctccgaaac accaacccta actttgttcg ttgtatcatt
     3361 ccaaatcacg agaagagggc tggaaaattg gatccacacc tagtcctaga tcagcttcgc
     3421 tgtaatggtg tcctggaagg gatccgaatc tgtcgccagg gcttccctaa ccgaatagtt
     3481 ttccaggaat tcagacagag atatgagatc ctaactccaa atgctattcc taaaggtttt
     3541 atggatggta aacaggcctg tgaacgaatg atccgggctt tagaattgga cccaaacttg
     3601 tacagaattg gacagagcaa gatatttttc agagctggag ttctggcaca cttagaggaa
     3661 gaaagagatt taaaaatcac cgatatcatt atcttcttcc aggccgtttg cagaggttac
     3721 ctggccagaa aggcctttgc caagaagcag cagcaactaa gtgccttaaa ggtcttgcag
     3781 cggaactgtg ccgcgtacct gaaattacgg cactggcagt ggtggcgagt cttcacaaag
     3841 gtgaagccgc ttctacaagt gactcgccag gaggaagaac ttcaggccaa agatgaagag
     3901 ctgttgaagg tgaaggagaa gcagacaaag gtggaaggag agctggagga gatggagcgg
     3961 aagcaccagc agcttttaga agagaagaat atccttgcag aacaactaca agcagagact
     4021 gagctctttg ctgaagcaga agagatgagg gcaagacttg ctgctaaaaa gcaggaatta
     4081 gaagagattc tacatgactt ggagtctagg gttgaagaag aagaagaaag aaaccaaatc
     4141 ctccaaaatg aaaagaaaaa aatgcaagca catattcagg acctggaaga acagctagac
     4201 gaggaggaag gggctcggca aaagctgcag ctggaaaagg tgacagcaga ggccaagatc
     4261 aagaagatgg aagaggagat tctgcttctc gaggaccaaa attccaagtt catcaaagaa
     4321 aagaaactca tggaagatcg cattgctgag tgttcctctc agctggctga agaggaagaa
     4381 aaggcgaaaa acttggccaa aatcaggaat aagcaagaag tgatgatctc agatctagaa
     4441 gaacgcttaa agaaggaaga aaagactcgt caggaactgg aaaaggccaa aagaaaactc
     4501 gacggggaga cgaccgacct gcaggaccag atcgcagagc tgcaggcgca gattgatgag
     4561 ctcaagctgc agctggccaa gaaggaggag gagctgcagg gcgcactggc cagaggtgat
     4621 gatgaaacac tccataagaa caatgccctt aaagttgtgc gagagctaca agcccaaatt
     4681 gctgaacttc aggaagactt tgaatccgag aaggcttcac ggaacaaggc cgaaaagcag
     4741 aaaagggact tgagtgagga actggaagct ctgaaaacag agctggagga cacgctggac
     4801 accacggcag cccagcagga actacgtaca aaacgtgaac aagaagtggc agagctgaag
     4861 aaagctcttg aggaggaaac taagaaccat gaagctcaaa tccaggacat gagacaaaga
     4921 cacgcaacag ccctggagga gctctcagag cagctggaac aggccaagcg gttcaaagca
     4981 aatctagaga agaacaagca gggcctggag acagataaca aggagctggc gtgtgaggtg
     5041 aaggtcctgc agcaggtcaa ggctgagtct gagcacaaga ggaagaagct cgacgcgcag
     5101 gtccaggagc tccatgccaa ggtctctgaa ggcgacaggc tcagggtgga gctggcggag
     5161 aaagcaagta agctgcagaa tgagctagat aatgtctcca cccttctgga agaagcagag
     5221 aagaagggta ttaaatttgc taaggatgca gctagtcttg agtctcaact acaggataca
     5281 caggagcttc ttcaggagga gacacgccag aaactaaacc tgagcagtcg gatccggcag
     5341 ctggaagagg agaagaacag tcttcaggag cagcaggagg aggaggagga ggccaggaag
     5401 aacctggaga agcaagtgct ggccctgcag tcccagttgg ctgataccaa gaagaaagta
     5461 gatgacgacc tgggaacaat tgaaagtctg gaagaagcca agaagaagct tctgaaggac
     5521 gcggaggccc tgagccagcg cctggaggag aaggcactgg cgtatgacaa actggagaag
     5581 accaagaacc gcctgcagca ggagctggac gacctcacgg tggacctgga ccaccagcgc
     5641 caggtcgcct ccaacttgga gaagaagcag aagaagtttg accagctgtt agcagaagag
     5701 aagagcatct ctgctcgcta tgccgaagag cgggaccggg ccgaagccga ggccagagag
     5761 aaagaaacca aagccctgtc actggcccgg gccctcgagg aagccctgga ggccaaggag
     5821 gagtttgaga ggcagaacaa gcagctccga gcagacatgg aagacctcat gagctccaaa
     5881 gatgatgtgg gaaaaaacgt tcacgaactt gaaaaatcca aacgggccct agagcagcag
     5941 gtggaggaaa tgaggaccca gctggaggag ctggaagacg aactccaggc cacggaagat
     6001 gccaagcttc gtctggaggt caacatgcag gccatgaagg cgcagttcga gagagacctg
     6061 caaaccaggg atgagcagaa tgaagagaag aagcggctgc tgatcaaaca ggtgcgggag
     6121 ctcgaggcgg agctggagga tgagaggaaa cagcgggcgc ttgctgtagc ttcaaagaaa
     6181 aagatggaga tagacctgaa ggacctcgaa gcccaaatcg aggctgcgaa caaagctcgg
     6241 gatgaggtga ttaagcagct ccgcaagctc caggctcaga tgaaggatta ccaacgtgaa
     6301 ttagaagaag ctcgtgcatc cagagatgag atttttgctc aatccaaaga gagtgaaaag
     6361 aaattgaaga gtctggaagc agaaatcctt caattgcagg aggaacttgc ctcatctgag
     6421 cgagcccgcc gacacgccga gcaggagaga gatgagctgg cggacgagat caccaacagc
     6481 gcctctggca agtccgcgct gctggatgag aagcggcgtc tggaagctcg gatcgcacag
     6541 ctggaggagg agctggaaga ggagcagagc aacatggagc tgctcaacga ccgcttccgc
     6601 aagaccactc tacaggtgga cacactgaac gccgagctag cagccgagcg cagcgccgcc
     6661 cagaagagtg acaatgcacg ccagcaactg gagcggcaga acaaggagct gaaggccaag
     6721 ctgcaggaac tcgagggtgc tgtcaagtct aagttcaagg ccaccatctc agccctggag
     6781 gccaagattg ggcagctgga ggagcagctt gagcaggaag ccaaggaacg agcagccgcc
     6841 aacaaattag tccgtcgcac tgagaagaag ctgaaagaaa tcttcatgca ggttgaggat
     6901 gagcgtcgac acgcggacca gtataaagag cagatggaga aggccaacgc tcggatgaag
     6961 cagcttaaac gccagctgga ggaagcagaa gaagaagcga cgcgtgccaa cgcatctcgg
     7021 cgtaaactcc agcgggaact ggatgatgcc accgaggcca acgagggcct gagccgcgag
     7081 gtcagcaccc tgaagaaccg gctgaggcgg ggtggcccca tcagcttctc ttccagccga
     7141 tctggccggc gccagctgca ccttgaagga gcttccctgg agctctccga cgatgacaca
     7201 gaaagtaaga ccagtgatgt caacgagacg cagccacccc agtcagagta aggtaccgcg
     7261 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     7321 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     7381 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7441 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7501 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     7561 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     7621 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7681 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7741 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7801 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     7861 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     7921 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8101 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     8161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8221 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8461 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     8521 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     8581 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     8641 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     8701 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     8761 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     8821 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     8881 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     8941 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     9001 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     9061 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     9121 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     9181 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     9241 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     9301 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     9361 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9421 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     9481 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     9541 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     9601 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     9661 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     9721 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     9781 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9841 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9901 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9961 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10021 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10081 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10141 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10201 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10261 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10321 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10381 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10441 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10501 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10561 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
    10621 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
