LOCUS       sequence-339588-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  large-stokes shift red fluorescent protein mBeRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Melzer S, Newmark ER, Mizuno GO, Hyun M, Philson AC, Quiroli E, 
            Righetti B, Gregory MR, Huang KW, Levasseur J, Tian L, Sabatini BL
  TITLE     Bombesin-like peptide recruits disinhibitory cortical circuits and 
            enhances fear memories.
  JOURNAL   Cell. 2021 Oct 28;184(22):5622-5634.e25. doi: 
            10.1016/j.cell.2021.09.013. Epub 2021 Oct 4.
  PUBMED    34610277
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09.013"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5622-5634.e25"
COMMENT     Sequence Label: pcDNA3.1-mBeRFP
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7..717
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERSTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRDY
                     MALKLVGGGHLICNAKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETSVEQHEVAV
                     ARYCDLPSKLGHKL"
     CDS             7..717
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERSTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRDY
                     MALKLVGGGHLICNAKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETSVEQHEVAV
                     ARYCDLPSKLGHKL"
ORIGIN
        1 gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac
       61 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc
      121 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
      181 ttcgacatcc tggctaccag cttcatgtac ggcagcaaga ccttcatcaa ccacacccag
      241 ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga gagatccacc
      301 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
      361 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag
      421 aagaaaacac tcggctggga ggccagcacc gagatgctgt accccgctga cggcggcctg
      481 gaaggcagag actacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacgcc
      541 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat
      601 gtggacagaa gactggaaag aatcaaggag gccgacaaag agacctccgt cgagcagcac
      661 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa actttaa
//
