LOCUS       sequence-339613-       10444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Nup62-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10444)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV Nup62-V5
FEATURES             Location/Qualifiers
     source          1..10444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5013..5054
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5191..5740
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5335..5352)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5562..5581
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5743..6342
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5743..5762)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6239..6259
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6366..6954
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6419..6439)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6837..6848)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7162..7795
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7924..7940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7924..7940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7937..7959)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7948..7964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7972..7978,7979..7996,7997..8002))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8017..8038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8155..8172)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8326..8911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8406..8425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9082..9873,9874..9942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9705..9724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9943..10047)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10095..10229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10132..10151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10186..10205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg agtggattta
     3421 actttggagg caccggggct cctgctggcg gctttacatt tgggaccgca aagactgcga
     3481 ccaccacacc tgccactggc ttttccttct ctgcttctgg caccggcacc ggagggttta
     3541 attttgggac tccctcccag ccagctgcga ccaccccttc caccagcctc ttctcactca
     3601 ccacacagac cccaaccaca cagaccccag gattcaactt tggaacaaca cctgcttctg
     3661 gaggaacagg cttctccctg gggatcagca ccccaaagct cagcctgagt aacgcagctg
     3721 ccacaccagc cacggccaac actggcagct ttgggcttgg cagcagcacc ctcaccaatg
     3781 ccatctcaag tggcagcacc tccaaccagg ggacagcccc cactggcttt gtctttggct
     3841 cttctaccac ctctgctccg tccaccggct ccactgggtt ctcattcacc agtggcagtg
     3901 catcccagcc tggagcctct ggcttcagcc ttggctctgt gggtagctct gcccagccca
     3961 cagcactgtc tggctctccc ttcacaccag ccactctggt gactactaca gcaggagcta
     4021 cacagccagc tgctgctgca cccactgctg ccaccaccag tgcagggtct acactctttg
     4081 cttccatagc tgctgctcct gcctcatcca gtgctacagg gctctccctc ccagctccgg
     4141 tgacaactgc agcaactcct agtgctggga ctttgggctt cagcctcaag gcccctggag
     4201 cagctcctgg tgcctccacc accagcacca ccactaccac taccaccact actaccactg
     4261 ctgctgccgc tgccgcctct accacaacca ctggctttgc cttaagtctg aaacccctgg
     4321 tgtcagctgg ccccagcagt gtagcagcta ctgctctgcc tgcctccagc acagcagctg
     4381 ggactgccac aggtccagcg atgacctatg cacagctgga aagcttgatc aacaagtgga
     4441 gtctggagct ggaagatcag gagcggcact tcctgcagca ggccacacag gtcaatgcct
     4501 gggaccgcac actgattgag aatggggaga agatcaccag cctgcaccga gaggtggaaa
     4561 aggtgaagct ggatcagaag cgactggacc aggagctgga ctttatcctg tcgcagcaga
     4621 aggagctgga agacctcctg agcccattgg aggagtcagt gaaggagcaa agtggcacca
     4681 tataccttca gcacgctgat gaggagcgtg agaagaccta caagctggct gagaacattg
     4741 atgctcagct caagcgcatg gcacaggacc tcaaggatat catcgagcac ctgaacatgg
     4801 ctggtggccc tgcagacacc agtgacccat tgcaacagat ctgcaagatc ctcaatgcac
     4861 acatggactc tcttcagtgg gtggaccaga gctctgccct gctgcagagg agggtggagg
     4921 aggctagccg tgtatgtgag ggccggcgca aggaacagga gcgcagtctg cgcattgcct
     4981 ttgacggtgg gccgggcggg ggtggcgggg gaggtaagcc tatccctaac cctctcctcg
     5041 gtctcgattc tacgtgagtt taaacttcga aacgcgtgaa ttcctcgagg gcggccgctc
     5101 tagagtcgac gggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc
     5161 tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     5221 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5281 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5341 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5401 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5461 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5521 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5581 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5641 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5701 accacgggga cgtggttttc ctttgaaaaa cacgataata ccatgaccga gtacaagccc
     5761 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5821 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5881 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5941 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6001 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6061 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6121 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6181 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6241 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6301 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacccgggcg gggcgcgtct
     6361 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6421 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6481 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6541 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6601 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6661 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6721 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6781 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6841 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6901 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6961 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7021 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gtttttccag
     7081 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7141 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7201 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7261 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7321 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     7381 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     7441 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     7501 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     7561 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     7621 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7681 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7741 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7801 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7861 gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc
     7921 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7981 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8041 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8101 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8161 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8221 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8281 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8341 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8401 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8461 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8521 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8581 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8641 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8701 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8761 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8821 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca
     8881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9061 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9121 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9181 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9241 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9301 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9361 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9421 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9481 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9541 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9601 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9661 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9721 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9781 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9841 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9901 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9961 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10021 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10081 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    10261 ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc
    10321 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    10381 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10441 tagt
//
