LOCUS       sequence-339636-        7821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Root Tip-Specific Expressing Anthocyanin Marker for Direct 
            Identification of Transgenic Tissues by the Naked Eye in Symbiotic 
            Studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ruan Y, Chen K, Su Y, Jiang S, Xu P, Murray JD
  TITLE     A Root Tip-Specific Expressing Anthocyanin Marker for Direct 
            Identification of Transgenic Tissues by the Naked Eye in Symbiotic 
            Studies.
  JOURNAL   Plants (Basel). 2021 Mar 23;10(3). pii: plants10030605. doi: 
            10.3390/plants10030605.
  PUBMED    33806858
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plants 
            (Basel)."; date: "2021-03-23"; volume: "10(3). pii: plants10030605. 
            doi"; pages: " 10.3390/plants10030605"
COMMENT     Sequence Label: pL1M-pMtRC-MtLAP1-Adh-R1
FEATURES             Location/Qualifiers
     source          1..7821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1149
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    1305..1329
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4928..4952
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      5044..5675
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5762..5866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5867..5935,5936..6727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6085..6104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6898..7486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7387..7406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc ggggttttcc
       61 gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc
      121 cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa
      181 ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg tcgtcttgaa
      241 caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac tatgacgacc
      301 aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc
      361 gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt gttcgatatt
      421 gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc
      481 accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc
      541 aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga tgacgaactg
      601 gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc
      661 acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta ttacacgaag
      721 gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt
      781 gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa
      841 acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac
      901 tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg acggatgttc
      961 gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg
     1021 tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa
     1081 gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct ggtgcattgc
     1141 aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagcagccag cgcctgatct
     1201 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc
     1261 cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
     1321 atcctgtcaa acactgatag tttaaaccac ttcgtgcaga agacaattgc ggagttgagt
     1381 gtacaaattc tgcaggcatg gctgttgttt tgtcttgtag cgtagacaga aaacagagta
     1441 gtggaggtag tggttgtaca cttgtactta gtcaactcta ttaacgagag acaagtgctc
     1501 agtgctatcc atatatccgt tgatacctcc ctttcaattc ttcgttcttc ttggataaga
     1561 ctgaattgcg aatcagctac tttgaatctt cagtttgatt aaaggatcaa acgtagcttc
     1621 tggtgaagat attgcttctg atgaaaactt ttgcttctga cgaccttctg catctgatga
     1681 cgtcattact tcagaagcac ttcagcttca ggagcagttt tcttcagatg ctcagaattt
     1741 cttttacatt ccattgttct tccaagacct attgaaataa cttgagtagc ctttggtcct
     1801 gtacacttga acaattatta gtaataacca attgacaatt tttaatacct tgttatcatc
     1861 aaaacttaat aaggttcatt gtgaaacaca ttttgttcca acacaattca caaaaactat
     1921 ttctgaattt atgcagggtg cataaggcat aaccgcaacc gggcaaaatg gggtgtccac
     1981 gaaacaatta tcaaataata aaacaagaaa attgaggttc attacaaata gagaaatgat
     2041 atttgtacaa ttatttttgg acaactctct ctcatactca catcatgttt ctattttctc
     2101 tttattgatt ttgtttgtgt taatacatca ttttcttcat aaaattttag ttgtctcaaa
     2161 aagggttaaa tatatttttg gtccctataa atataccaac ttttcgtttt agtccctcta
     2221 aaaaatttct tcaactttta gtccctataa aattttcaat cactactttt gatccctatt
     2281 tttaagttta ctttagtatt ttttaatgaa attgtgcaga tatgtgtaga ataatgtaaa
     2341 aatatttccc aaaacaaatt agaatttttt aactaaacat aaatttaata tgaattttaa
     2401 ccatcaaaaa tataaaaatt catattaaat tcatgttttg ttaaaaaatt ctaaattttt
     2461 ttgagagaga ttcttttaat attatgaatt tttctgtaaa atcttattca aaaatatgaa
     2521 ttctacatga gttaggatgg accaaaagtg aagatggaaa atttttgagg gactaaaagt
     2581 tgaaggaaaa ttttataggg actaaaacaa aaggttgata tatttatagg gaccaaaaac
     2641 atatttaacc ctctcaaaaa ttgtatataa aaggttgttc aaataacaca actcttataa
     2701 taaagctgtg atataagtac actcacgctt taagtacacc attttaattt ttaacaccat
     2761 gtatacgtga cagttttgtt ttgtctcatc taaccaaaaa ataaaataaa ataaaataaa
     2821 tagaaatgtc tttaagtcat tgtttaaaca ttcaaaagta gtacctttac attaaaaatt
     2881 gtataaacat gacttttttt taatgtaatt atagtaatca aactatatat tgatcactat
     2941 tattgaaaag gaaaaatcca ttgaaaaaca aatgaatgaa agatgctttc aaattttaat
     3001 acttgcatgc caattcaact tatcaaatga gtttgaatga aaagatgctt ttaagatgca
     3061 tcaataacta tgatgtgcgg ttgacttgta tgtaaagtag tcgttggtgg agctgttcag
     3121 aacaatataa agttcacatg ccatcaattc aggctttctt gttatcaact ctacacataa
     3181 aatcacgtta ctaaagctaa attcattgct aatactctca ccggcaaagg ttacaataag
     3241 cgaatcttaa gtcacaatct acaaataaat tagttcttca acattatcgt agtcataaat
     3301 tagttggcac ttttaacact attaagcttt gaatagccct catatatatc atcatatcaa
     3361 gcaaccacaa actttagaag cgtatttaga aaaaagatac aaacaaatct taagccgaac
     3421 ttctagctct aggccaacat gcatatacca aattctctct atatattttc ttcaatacat
     3481 gtatctatcc atcaatctat aaatcctcta catcatagaa gtaattgaaa atctctaaat
     3541 aagcaaagga aagtaataaa agataaaatc aagagagttc accaagaatg atggagaata
     3601 ccggaggtgt gagaaaaggc gcatggactt acaaggaaga tgagctactc aaggcttgca
     3661 ttaacacgta cggtgaagga aaatggaatt tagttcctca gagatctggt atggtatatg
     3721 ttcttattct actatttatt tggcttatag ttctagttta gttgaaaccc tatattggag
     3781 caaaccctat aacgatgcat taattcttta acataaatat cttggcaaaa tttgttacac
     3841 ttgacacaca ggattgaata gatgcagaaa aagttgtaga ttgaggtggt taaattactt
     3901 aagccccaac atcaacagag gaagattttc tgaggatgaa gaagatttga tcctaaggtt
     3961 acacaaacta ctaggaaata ggtaatctta catagttaaa ttgaaattaa agttgtttct
     4021 gtgtgctagc tgctgatcta tttcagctat ttacttactg atatacatat gtatatagat
     4081 ggtcattgat tgctggaagg cttccgggta gaacagctaa tgatgtgaag aactattggc
     4141 acacaaattt ggcaaagaaa gtggtttcag aaaaggaaga agagaaagaa aacgataaac
     4201 ctaaggaaac catgaaagct catgaagtta ttaaacctcg tcctataact ttgtcaagtc
     4261 attcaaattg gttgaagggg aaaaatagta ttcctagaga tcttgattac tcagagaata
     4321 tggcttcaaa tcaaattggt agagagtgtg cttctacttc aaaaccagat ctaggtaatg
     4381 ccccaatacc atgtgaaatg tggtgtgaca gtttgtggaa cttgggagaa catgtagaca
     4441 gtgagaaaat tgggtcatgc tcttcattac aagaggagaa cttaatggag tttccaaatg
     4501 ttgatgatga ctccttttgg gatttcaacc tttgtgattt gaattctctt tgggatctac
     4561 cttgagctta gccattctct cgcagatgat gttcactttc tgttttactt cctttatgca
     4621 ttcacagcaa taaaagaaag aaatctccat cgcttttggt tttcttctct gtcttaagtt
     4681 agtcgttttc gtgtctaatc tattacttat cattgtaata gactcttctt ctattgagat
     4741 ttgaatataa actaaaacac attccatttt actgtgttct caacattcag aatgcaaacg
     4801 gactaaccgt agtgactcgc gctggatccg aattcggcat tgtcttcaca gagtggggcc
     4861 cactgcatcc accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     4921 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     4981 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtcagatca ggatctcctt
     5041 tgcgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
     5101 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg
     5161 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
     5221 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
     5281 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca
     5341 gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
     5401 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
     5461 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
     5521 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
     5581 tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg
     5641 gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg
     5701 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gccaattcgt
     5761 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5821 acaataacct tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6541 tggctggttt attgctgata aatctggagc cggtgagcgt ggttctcgcg gtatcattgc
     6601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6781 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7021 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7501 ggccttttta cggttcctgg cagatcctag atgtggcgca acgatgccgg cgacaagcag
     7561 gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc ccacggcaag
     7621 tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac caagtacgag
     7681 aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga ttatctggac
     7741 accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc gtgagtcggg
     7801 gcaatcccgc aaggagggtg a
//
