LOCUS       sequence-33965-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pLKO mouse ULK2 shRNA 93
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    127..244
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        293..803
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     722..740
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(825..844)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tttatatatc ttgtggaaag gacgaaacac cggcgccatc tgaacatgat gttactcgag
       61 taacatcatg ttcagatggc gtttttaatt ctcgacctcg agacaaatgg cagtattcat
      121 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      181 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      241 ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg
      301 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
      361 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
      421 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
      481 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
      541 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
      601 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
      661 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
      721 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
      781 gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc gagtacaagc
      841 ccacggtgcg cctcgccacc cgcgacgac
//
