LOCUS       sequence-339655-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3xT7-tagged constitutive active form of ChREBP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Jang Y, Park YK, Lee JE, Wan D, Tran N, Gavrilova O, Ge K
  TITLE     MED1 is a lipogenesis coactivator required for postnatal adipose 
            expansion.
  JOURNAL   Genes Dev. 2021 Apr 22. pii: gad.347583.120. doi: 
            10.1101/gad.347583.120.
  PUBMED    33888555
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2021 Apr 22. pii: gad.347583.120. doi: 10.1101/gad.347583.120."
COMMENT     Sequence Label: pMSCVhyg-3xT7-CA-ChREBP
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1427..1436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1571..1576,1577..1600,1601..1636,1637..1660)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     promoter        3582..4081
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3617..3640)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4014..4033
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4099..5124
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4099..5124
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             5482..5996
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6158..6174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6182..6188,6189..6206,6207..6212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6227..6248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6365..6382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6536..7124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6616..6635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7295..8086,8087..8155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7918..7937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8156..8260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8328..8346
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8384..8406)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8506..8525
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8719..8741
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8869..8888
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctctc gacgaggcca ccatggcttc
     1441 tatgaccggc gggcagcaaa tgggcggctc ttcggccagc atggcttcta tgaccggtgg
     1501 ccaacagatg ggtggttcct cagctagaat ggcgagtatg accggtggcc aacagatggg
     1561 tggtagtgga agcgcgtgga gccaccctca gttcgagaag ggaggaggaa gcggcggagg
     1621 cagcggagga agcgcttgga gccacccgca gttcgagaaa ggagctagat cacccgggct
     1681 cgagcggcag cttctagacc tggactgctt cttgtccgat atctccgaca cactcttcac
     1741 catgacacag cccagtcctt cgtccctgca gctgccccca gaagatgctt atgttggcaa
     1801 tgctgacatg atccagccag acctgacgcc gctgcagccc agcctagatg acttcatgga
     1861 gatatcagat ttcttcaccg attaccgccc ccctcagaca cccacatctt caaactacat
     1921 agagtccccc agctttggcc ccatggctga ctccctcttc agcagtggga tcctggcccc
     1981 agagatgcca tctccagcct cgtcttcctc ctcctcaggg atgacccctc actcagggaa
     2041 tacacgccta caggctcgga acagctgctc tggacccttg gatcccaacc cctttctgag
     2101 ctctgaattc cttcttcctg aagaccctaa gaccaagatc ccacctgctc ctggaccgac
     2161 ccctctcctt ccattcccca cccctgtcaa ggtgcatggc ttagagccct gcaccccctc
     2221 tcccttcccc acaatggctc cgcccccctc tttgttgcca gaagaatctc tcctctctgc
     2281 caggttccca ttcacctcag gccctcctgc tccaggagtg tctacgcttc ctgcacccac
     2341 aacctttgtc cccaccccac agcccggccc tggtcccgtc cccttctctg tagaccatct
     2401 gccccatggg tacctggaac ccgtctttgg gcctcacttc actgtgcctc agggtatgca
     2461 acccaggtgc aagccctcct ccccatcccc tgggggacaa aaagcaagtc cacccacctt
     2521 ggcctctgcc accgccagtc ccactgccac tgccactgcc agagacaaca acccctgtct
     2581 tacacagctg ctcaggcttg gctgccctga gcaagcactg gagcctccga ctatgcccgg
     2641 taccctcctc cggcctccag agtccccgca ggatacagtt tctgaaatcc cccgtgcccg
     2701 tgcctttttc cccccaatcc cggcccctac accaccccgg ccacctccag gcccagccac
     2761 attggctcct cccaggtccc tggttgtccc caaagcagag cggctctcgc ctccagcctc
     2821 cagcggcagt gagcggcgac tatcagggga tctcaactcc atacaaccct cgggggcact
     2881 gagtgtccac ctgtctcccc ctcaaactgt cctaagccgg ggtcgtgtag acaacaacaa
     2941 gatggagaac cgacgtatca cacacatctc cgcggagcag aagaggcggt tcaatattaa
     3001 gctaggattc gacaccctgc acggacttgt cagcacgctc agtgcccagc ccagcctcaa
     3061 ggtgagcaaa gcaaccacgc ttcagaagac agcggagtac atcctgatgc tgcagcagga
     3121 acgggcagct atgcaggagg aggcgcagca gctgcgggat gaaatagagg agctcaacgc
     3181 tgccatcaac ttgtgccagc agcagctacc ggccaccggg gtgcccatca cacatcagcg
     3241 ctttgaccag atgcgggaca tgtttgatga ctatgtccgg acgcgcacgc tgcacaactg
     3301 gaagttctgg gtgttcagca tcctcatccg acctttattt gaatccttca atgggatggt
     3361 gtctaccgcc agcttgcaca gcctccgcca gacctcactg gcctggctgg aacagtactg
     3421 ttccctacct gctctccggc caactgtcct gaattccctt cgccaactca gcacttccac
     3481 aagcatcctg actgacccca gccttgtgcc tgagcaagcc acacgggcag tcacagaggg
     3541 caccctgggg agaccattat aatgagttaa cgaattctac cgggtagggg aggcgctttt
     3601 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     3661 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     3721 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     3781 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     3841 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     3901 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     3961 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     4021 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     4081 gacctgcatc ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     4141 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     4201 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     4261 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     4321 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     4381 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     4441 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     4501 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     4561 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     4621 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     4681 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     4741 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     4801 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     4861 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     4921 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     4981 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     5041 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     5101 actcgtccga gggcaaagga atagagtaga tgccgaccga acaagagctg atttcgagaa
     5161 cgcctcagcc agcaactcgc gcgagcctag caaggcaaat gcgagagaac ggccttacgc
     5221 ttggtggcac agttctcgtc cacagttcgc taagctcgct cggctgggtc gcgggagggc
     5281 cggtcgcagt gattcaggcc cttctggatt gtgttggtcc ccagggcacg attgtcatgc
     5341 ccacgcactc gggtgatctg actgatcccg cagattggag atcgccgccc gtgcctgccg
     5401 attgggtgca gatccgtcga cctgcagcca agcttatcga taaaataaaa gattttattt
     5461 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     5521 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     5581 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     5641 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     5701 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     5761 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     5821 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     5881 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     5941 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     6001 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     6061 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     6121 atggacagcg cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac
     6181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8281 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8341 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     8401 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     8461 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     8521 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     8581 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     8641 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     8701 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc
     8761 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     8821 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     8881 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     8941 gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     9001 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     9061 tttatttagt ctccagaaaa agggggg
//
