LOCUS       sequence-33966-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pcdna3 flag - ULK1 wt
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct gccaccatgg actacaagga cgacgacgat aaggaattcg
      121 ttatcacaag tttgtacaaa aaagcaggct tcgagccggg ccgaggcggc gtcgagaccg
      181 tgggcaagtt cgagttctct cgcaaggacc tgattggaca cggcgccttc gcggtggtct
      241 tcaagggtcg acaccgcgag aagcacgacc tggaggtggc cgtcaaatgc attaacaaga
      301 agaaccttgc caagtcccaa acactgctgg gaaaggaaat caaaatcctg aaggaactaa
      361 agcacgaaaa catcgtggcg ctgtatgact tccaggaaat ggctaattct gtctacctgg
      421 tcatggagta ttgtaatggt ggagacctgg ctgactacct gcacactatg cgcacactga
      481 gtgaagacac tgtcaggctt ttcctacagc agatcgctgg cgccatgcgg ctgctgcaca
      541 gcaagggcat catccaccgg gacctgaagc cccaaaacat cctgctgtcc aaccctgggg
      601 gccgccgggc caaccccagc aacatccgag tcaagattgc tgactttgga ttcgctcggt
      661 acctccagag caacatgatg gcggccacac tctgtggttc tcctatgtac atggctcctg
      721 aggtcattat gtcccagcac tacgatggaa aggctgacct gtggagcatt ggcaccattg
      781 tctaccagtg tct
//
