LOCUS       sequence-339678-        5032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a positive control reporter with 3' UTR sequences perfectly 
            complementary to miR-124.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Li P, Chen Y, Juma CA, Yang C, Huang J, Zhang X, Zeng Y
  TITLE     Differential Inhibition of Target Gene Expression by Human 
            microRNAs.
  JOURNAL   Cells. 2019 Jul 30;8(8). pii: cells8080791. doi: 
            10.3390/cells8080791.
  PUBMED    31366019
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2019-07-30"; volume: "8(8). pii: cells8080791. doi"; pages: "
            10.3390/cells8080791"
COMMENT     Sequence Label: pCMV-luc-miR-124
FEATURES             Location/Qualifiers
     source          1..5032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     256..275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1019..1398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1403..1606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1557..1577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1676..3328
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1742..1760)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(3701..3719)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3787..3891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3892..3960,3961..4752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4110..4129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
       61 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      361 cagcaacgga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
      421 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
      481 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
      541 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
      601 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
      661 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
      721 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
      781 gagaagaatc ataatgggga aggccatcca gcctcgcgtc ggggagcttt ttgcaaaagc
      841 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
      901 cggcctctgc ataaataaaa aaaattagtc agccatgagc ttggcccatt gcatacgttg
      961 tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga
     1021 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1081 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1141 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1201 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1261 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1321 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1381 gtcatcgcta ttaccatgca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1441 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1501 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1561 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaagtc
     1621 aagcttaagt gaccagctac agtcggaaac catcagcaag aggtcattgt tcaccatgga
     1681 agacgccaaa aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc
     1741 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     1801 tacagatgca catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg
     1861 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     1921 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     1981 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca
     2041 gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     2101 attaccaata atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca
     2161 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     2221 accagagtcc tttgatcgtg acaaaacaat tgcactgata atgaattcct ctggatctac
     2281 tgggttacct aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc
     2341 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     2401 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     2461 cgtcttaatg tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat
     2521 tcaaagtgcg ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga
     2581 caaatacgat ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga
     2641 agtcggggaa gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct
     2701 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     2761 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     2821 gggcgttaat cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt
     2881 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     2941 catagcttac tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat
     3001 taaatacaaa ggatatcagg tggcccccgc tgaattggaa tcgatattgt tacaacaccc
     3061 caacatcttc gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc
     3121 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     3181 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     3241 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     3301 caagaagggc ggaaagtcca aattgtaact ctagacaaga ggtcattgtt caccatgggt
     3361 tgatccgcta gcggcattca ccgcgtgcct taatggcatt caccgcgtgc cttactcgag
     3421 tgaccttcag accttggcac tggaggtggc ccggcagaag cgcggcatcg tggatcagtg
     3481 ctgcaccagc atctgctctc tctaccaact ggagaactac tgcaactagg cccaccacta
     3541 ccctgtccac ccctctgcaa tgaataaaac ctttgaaaga gcactacaag ttgtgtgtac
     3601 atgcgtgcat gtgcatatgt ggtgcggggg gaacatgagt ggggctggct ggagtggcga
     3661 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     3721 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3841 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3901 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3961 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4021 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4081 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     4141 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4201 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4261 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4321 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4381 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     4441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4801 catttttaat ttaaaaggat ctaggctgct gcttgcaaac aaaaaaacca ccgctaccag
     4861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4921 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gt
//
