LOCUS       sequence-339683-        5033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a positive control reporter with 3' UTR sequences perfectly 
            complementary to miR-122.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Li P, Chen Y, Juma CA, Yang C, Huang J, Zhang X, Zeng Y
  TITLE     Differential Inhibition of Target Gene Expression by Human 
            microRNAs.
  JOURNAL   Cells. 2019 Jul 30;8(8). pii: cells8080791. doi: 
            10.3390/cells8080791.
  PUBMED    31366019
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2019-07-30"; volume: "8(8). pii: cells8080791. doi"; pages: "
            10.3390/cells8080791"
COMMENT     Sequence Label: pCMV-luc-miR-122
FEATURES             Location/Qualifiers
     source          1..5033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        438..641
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     592..612
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             711..2363
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(777..795)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(2737..2755)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2823..2927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2928..2996,2997..3788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3146..3165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4324..4343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg
       61 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      421 cgctattacc atgcatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      481 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      541 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      601 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aagtcaagct
      661 taagtgacca gctacagtcg gaaaccatca gcaagaggtc attgttcacc atggaagacg
      721 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
      781 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
      841 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
      901 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
      961 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     1021 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     1081 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     1141 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     1201 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     1261 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     1321 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     1381 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     1441 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     1501 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     1561 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     1621 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     1681 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     1741 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     1801 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     1861 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     1921 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     1981 cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     2041 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     2101 tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg
     2161 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     2221 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     2281 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     2341 agggcggaaa gtccaaattg taactctaga caagaggtca ttgttcacca tgggttgatc
     2401 cgctagcaaa caccattgtc acactccaca aacaccattg tcacactcca ctcgagtgac
     2461 cttcagacct tggcactgga ggtggcccgg cagaagcgcg gcatcgtgga tcagtgctgc
     2521 accagcatct gctctctcta ccaactggag aactactgca actaggccca ccactaccct
     2581 gtccacccct ctgcaatgaa taaaaccttt gaaagagcac tacaagttgt gtgtacatgc
     2641 gtgcatgtgc atatgtggtg cggggggaac atgagtgggg ctggctggag tggcgatgat
     2701 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     2761 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     2821 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2881 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2941 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3001 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3061 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3121 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     3181 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3241 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3301 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3361 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3421 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     3481 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3541 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3601 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3661 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3721 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3781 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3841 tttaatttaa aaggatctag gctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3961 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacggaga
     4441 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     4501 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     4561 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     4621 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     4681 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     4741 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     4801 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     4861 aatggggaag gccatccagc ctcgcgtcgg ggagcttttt gcaaaagcct aggcctccaa
     4921 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
     4981 aaataaaaaa aattagtcag ccatgagctt ggcccattgc atacgttgta tcc
//
