LOCUS       sequence-339753-        5348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2
            - EGFP fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Schanda K, Peschl P, Lerch M, Seebacher B, Mindorf S, Ritter N, 
            Probst M, Hegen H, Di Pauli F, Wendel EM, Lechner C, Baumann M, 
            Mariotto S, Ferrari S, Saiz A, Farrell M, Leite MIS, Irani SR, 
            Palace J, Lutterotti A, Kumpfel T, Vukusic S, Marignier R, Waters P,
            Rostasy K, Berger T, Probst C, Hoftberger R, Reindl M
  TITLE     Differential Binding of Autoantibodies to MOG Isoforms in 
            Inflammatory Demyelinating Diseases.
  JOURNAL   Neurol Neuroimmunol Neuroinflamm. 2021 Jun 15;8(5). pii: 8/5/e1027. 
            doi: 10.1212/NXI.0000000000001027. Print 2021 Jul.
  PUBMED    34131067
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurol 
            Neuroimmunol Neuroinflamm."; date: "2021-06-15"; volume: "8(5). pii:
            8/5/e1027. doi"; pages: " 10.1212/NXI.0000000000001027. Print 2021 
            Jul"
COMMENT     Sequence Label: pEGFP-N1 hMOG-alpha2-EGFP
FEATURES             Location/Qualifiers
     source          1..5348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        123..314
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     271..291
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(316..420)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      447..902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(534..553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     744..765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    909..1030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(920..939)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     974..993
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(1153..1866,1867..1869,1870..1872))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1156..1866,1867..1869,1870..1872))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1198..1219)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1547..1566
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1806..1827
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1869..1878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2598..2801)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2627..2647)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2802..3105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3280..3868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3360..3379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4197..4244
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4269..4288
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4476..5270)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4476..5270)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4587..4606)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5197..5216
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(5305..5348,1..314))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5319..5348,1..106)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
       61 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
      121 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
      181 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
      241 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
      301 tctttccgcc tcaggactct tcctttttca atattattga agcatttatc agggttattg
      361 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      421 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
      481 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      541 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      601 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      661 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      721 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      781 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
      841 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
      901 ttacgcctta agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      961 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1021 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1081 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga
     1141 gtcgcggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     1201 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     1261 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     1321 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     1381 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     1441 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     1501 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     1561 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     1621 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     1681 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     1741 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     1801 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     1861 cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg actgcagaat
     1921 tcggaaggga tttcgtagct cttcaaggaa ttgcccaaaa gtccggtgga gattctctat
     1981 ctctgctcga agttttcctc tcagtctgta ctgcaggcag aggaagacga ggccaacagt
     2041 gatctgcagg aggagcacag gcagcaccgc gaggagaacc agcactccag ggctcaccca
     2101 gtagaaagga tcttctactt tcaattccat tgctgcctcc tcttggtaag aatgatctcg
     2161 gaagaagcag gtgaaacctc cttcatctga gaaccttaca ttccggatcc tgagagtcac
     2221 ctttccctca ccaatagcat ctttcagcag ctctgtccgg ccccgatatt caggtgcctg
     2281 gtctccatct tggtccttgc catttctgta gagatgaacc accctagaga agggggggcg
     2341 gtaccacccc acctccatgc ctgtagcgtt cttcccagga gatatgcgac atggcaattc
     2401 cacttcatcc ccgaccagag cccggatagg gtgtcttggt cctatcactc tgaactgccc
     2461 tgcatagctg gaagacactt ggaggaggag gaggaggagg aaggagcaga ggcagctggg
     2521 cagagagggt cttgataagc ttgccatctc gagatctgag tccggtagcg ctagcggatc
     2581 tgacggttca ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc
     2641 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
     2701 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     2761 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
     2821 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     2881 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     2941 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     3001 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     3061 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     3121 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactaatgca tggcggtaat
     3181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3721 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3961 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4021 gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc
     4081 acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg
     4141 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
     4201 aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc
     4261 ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc
     4321 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     4381 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     4441 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4501 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4561 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4621 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4681 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
     4741 ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4801 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4861 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4921 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     4981 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     5041 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc
     5101 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     5161 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     5221 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     5281 cctcatcctg tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca
     5341 ctacttct
//
