LOCUS       sequence-339756-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression and purification, mixed with polyPRM to form 
            condensates, can bind FKBP in the presence of rapamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: FRB-polySH33
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..285
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
ORIGIN
        1 ggcgaattca tgttagagat gtggcatgaa ggacttgagg aagcttcccg cctttacttt
       61 ggtgaacgta atgttaaggg catgttcgaa gtattggaac ccctgcacgc gatgatggaa
      121 cgtgggccac agacattgaa agaaacaagc tttaaccaag cttacggacg tgatctgatg
      181 gaggcgcaag agtggtgccg taagtatatg aagtccggta acgtaaaaga cctgacacaa
      241 gcatgggatt tgtactacca tgtttttcgc cgtatctcga agcaagttga tggaggtagt
      301 ggcggatcag gtgggagtgg agggtctcat atggacctca acatgcccgc ttatgtgaaa
      361 tttaactaca tggctgagag agaggatgaa ttatcattga taaaggggac aaaggtgatc
      421 gtcatggaga aaagcagtga tgggtggtgg cgtggtagct acaatggaca agttggatgg
      481 ttcccttcaa actatgtaac tgaagaaggt gacagtcctt tggcgtctgg tgcgggtggt
      541 tctgaaggtg gtggttctga aggtggtacc tctggtgcga ccgacctcaa catgcccgct
      601 tatgtgaaat ttaactacat ggctgagaga gaggatgaat tatcattgat aaaggggaca
      661 aaggtgatcg tcatggagaa aagcagtgat gggtggtggc gtggtagcta caatggacaa
      721 gttggatggt tcccttcaaa ctatgtaact gaagaaggtg acagtccttt ggcatccgga
      781 gcaggaggct ccgaaggagg aggctccgag ggaggaacat ccggagcaac agacctcaac
      841 atgcccgctt atgtgaaatt taactacatg gctgagagag aggatgaatt atcattgata
      901 aaggggacaa aggtgatcgt catggagaaa agcagtgatg ggtggtggcg tggtagctac
      961 aatggacaag ttggatggtt cccttcaaac tatgtaactg aagaaggtga cagtcctttg
     1021 ggaggcggat ccgaaaacct gtattttcag
//
