LOCUS       sequence-339760-        1332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression and purification, low affinity SUMOylation 
            substrate, point mutation F562A reduces affinity for E2, increasing 
            the Km.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: EGFP-RanGAP*
FEATURES             Location/Qualifiers
     source          1..1332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(10..12,13..723)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDLNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(313..332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     660..681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcgaattca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acttaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aagtccggac tcagatctcc tcagcagcga gggcagggag agaagtcagc cacgccctca
      781 cggaagattc tggaccctaa cactggggag ccagctcccg tgctgtcctc cccacctcct
      841 gcagacgtct ccaccttcct ggcttttccc tctccagaga agctgctgcg cctagggccc
      901 aagagctccg tgctgatagc ccagcagact gacacgtctg accccgagaa ggtggtgtct
      961 gccttcctaa aggtgtcatc tgtgttcaag gacgaagcta ctgtgaggat ggcagtgcag
     1021 gatgcagtag atgccctgat gcagaaggct ttcaactcct cgtccttcaa ctccaacacc
     1081 ttcctcacca ggctcctcgt gcacatgggt ctgctcaaga gtgaagacaa ggtcaaggcc
     1141 attgccaacc tgtacggccc cctgatggcg ctgaaccaca tggtgcagca ggactatttc
     1201 cccaaggccc ttgcacccct gctgctggcg gccgtgacca agcccaacag cgccctggaa
     1261 tcctgctcct tcgcccgcca cagtctgctg cagacgctgt acaaggtcgg atccgaaaac
     1321 ctgtattttc ag
//
