LOCUS       sequence-339761-        1695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression and purification, low affinity SUMOylation 
            substrate that can recruited to FRB with rapamycin, point mutation 
            F562A reduces affinity for E2, increasing the Km.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: FKBP-EGFP-RanGAP*
FEATURES             Location/Qualifiers
     source          1..1695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(10..12,13..330)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKL"
     CDS             join(373..375,376..1086)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(415..436)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(676..695)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1023..1044
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcgaattca tgggggtaca agttgaaaca attagccctg gggacggacg tactttccca
       61 aaacgcggtc agacctgtgt ggtccactat actgggatgc ttgaggacgg taagaaattc
      121 gactcaagtc gtgaccgtaa caaacccttc aagttcatgt taggtaaaca ggaagtgatt
      181 cgtgggtggg aggaaggagt ggcccagatg tcagtaggcc aacgtgctaa gttgacaatt
      241 tcgcccgact acgcctacgg agcaacgggt catccgggaa ttattccacc acacgcaaca
      301 ttagtattcg acgtcgagtt gttgaaattg aacgagggag gtagtggcgg atcaggtggg
      361 agtggagggt ctgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      421 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      541 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      601 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      661 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      721 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      781 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      841 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      901 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      961 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1021 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1081 tacaagtccg gactcagatc tcctcagcag cgagggcagg gagagaagtc agccacgccc
     1141 tcacggaaga ttctggaccc taacactggg gagccagctc ccgtgctgtc ctccccacct
     1201 cctgcagacg tctccacctt cctggctttt ccctctccag agaagctgct gcgcctaggg
     1261 cccaagagct ccgtgctgat agcccagcag actgacacgt ctgaccccga gaaggtggtg
     1321 tctgccttcc taaaggtgtc atctgtgttc aaggacgaag ctactgtgag gatggcagtg
     1381 caggatgcag tagatgccct gatgcagaag gctttcaact cctcgtcctt caactccaac
     1441 accttcctca ccaggctcct cgtgcacatg ggtctgctca agagtgaaga caaggtcaag
     1501 gccattgcca acctgtacgg ccccctgatg gcgctgaacc acatggtgca gcaggactat
     1561 ttccccaagg cccttgcacc cctgctgctg gcggccgtga ccaagcccaa cagcgccctg
     1621 gaatcctgct ccttcgcccg ccacagtctg ctgcagacgc tgtacaaggt cggatccgaa
     1681 aacctgtatt ttcag
//
