LOCUS       sequence-339765-        1251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression and purification, E2 enzyme (UBC9) for 
            SUMOylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: mCherry-E2
FEATURES             Location/Qualifiers
     source          1..1251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..714
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(152..170)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     420..439
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     614..637
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ggcgaattcg tgagcaaggg cgaggaggac aacatggcca tcatcaagga gttcatgcgc
       61 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      121 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
      181 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      241 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      301 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      361 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      421 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      481 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      541 gacgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      601 aacgtcaaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
      661 tacgagcgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccgga
      721 ggaggtagtg gcggatcagg tgggagtgga gggtctatgt cggggatcgc cctcagcaga
      781 ctcgcccagg agaggaaagc atggaggaaa gaccacccat ttggtttcgt ggctgtccca
      841 acaaaaaatc ccgatggcac gatgaacctc atgaactggg agtgcgccat tccaggaaag
      901 aaagggactc cgtgggaagg aggcttgttt aaaatacgga tgcttttcaa agatgattat
      961 ccatcttcgc caccaaaatg taaattcgaa ccaccattat ttcacccgaa tgtgtaccct
     1021 tcggggacag tgtgcctgtc catcttagag gaggacaagg actggaggcc agccatcaca
     1081 atcaaacaga tcctattagg aatacaggaa cttctaaatg aaccaaatat ccaagaccca
     1141 gctcaagcag aggcctacac gatttactgc caaaacagag tggagtacga gaaaagggtc
     1201 cgagcacaag ccaagaagtt tgcgccctca ctcgagaacc tgtattttca g
//
