LOCUS       sequence-339767-        1632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression and purification, E2 enzyme (UBC9) for 
            SUMOylation, can be recruited to FRB with rapamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: CyPet-FKBP-E2
FEATURES             Location/Qualifiers
     source          1..1632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(313..332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     660..681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(730..732,733..1050)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKL"
ORIGIN
        1 ggcgaattcg tgagcaaggg cgaggagctg ttcggcggga ttgtgcccat cctggtcgag
       61 ctggaaggcg acgtaaacgg ccacaagttc tcggtatccg gggagggaga gggagacgct
      121 acctatggga aacttacatt aaaatttatt tgcacgacag ggaagctgcc ggtgccttgg
      181 cctactctgg ttaccaccct gacatggggc gtccaatgtt tcagccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgtg atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctatatcagc cataacgtct atatcaccgc cgacaagcag
      481 aagaacggca tcaaggccaa cttcaaggct cgccacaaca tcaccgacgg gtcggttcaa
      541 ctggccgacc actatcagca aaatacacct attggcgacg gccccgtgat tctgcccgac
      601 aaccactacc tgagcaccca gtccgcgctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc atggcatgga cgagctgtac
      721 aagcaattga tgggggtaca agttgaaaca attagccctg gggacggacg tactttccca
      781 aaacgcggtc agacctgtgt ggtccactat actgggatgc ttgaggacgg taagaaattc
      841 gactcaagtc gtgaccgtaa caaacccttc aagttcatgt taggtaaaca ggaagtgatt
      901 cgtgggtggg aggaaggagt ggcccagatg tcagtaggcc aacgtgctaa gttgacaatt
      961 tcgcccgact acgcctacgg agcaacgggt catccgggaa ttattccacc acacgcaaca
     1021 ttagtattcg acgtcgagtt gttgaaattg aacgagggag gtagtggcgg atcaggtggg
     1081 agtggagggt ctctgagaag ccgtgctcag gcttcgaact ctgcagttga cggcaccatg
     1141 tcggggatcg ccctcagcag actcgcccag gagaggaaag catggaggaa agaccaccca
     1201 tttggtttcg tggctgtccc aacaaaaaat cccgatggca cgatgaacct catgaactgg
     1261 gagtgcgcca ttccaggaaa gaaagggact ccgtgggaag gaggcttgtt taaactacgg
     1321 atgcttttca aagatgatta tccatcttcg ccaccaaaat gtaaattcga accaccatta
     1381 tttcacccga atgtgtaccc ttcggggaca gtgtgcctgt ccatcttaga ggaggacaag
     1441 gactggaggc cagccatcac aatcaaacag atcctattag gaatacagga acttctaaat
     1501 gaaccaaata tccaagaccc agctcaagca gaggcctaca cgatttactg ccaaaacaga
     1561 gtggagtacg agaaaagggt ccgagcacaa gccaagaagt ttgcgccctc actcgagaac
     1621 ctgtattttc ag
//
