LOCUS       sequence-339770-        1362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression and purification, low affinity SUMOylation 
            substrate with a relatively high Km, can be recruited to FRB with 
            rapamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: FKBP-EGFP-PML peptide
FEATURES             Location/Qualifiers
     source          1..1362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(10..12,13..330)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKL"
     CDS             join(373..375,376..1086)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(415..436)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(676..695)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1023..1044
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcgaattca tgggggtaca agttgaaaca attagccctg gggacggacg tactttccca
       61 aaacgcggtc agacctgtgt ggtccactat actgggatgc ttgaggacgg taagaaattc
      121 gactcaagtc gtgaccgtaa caaacccttc aagttcatgt taggtaaaca ggaagtgatt
      181 cgtgggtggg aggaaggagt ggcccagatg tcagtaggcc aacgtgctaa gttgacaatt
      241 tcgcccgact acgcctacgg agcaacgggt catccgggaa ttattccacc acacgcaaca
      301 ttagtattcg acgtcgagtt gttgaaattg aacgagggag gtagtggcgg atcaggtggg
      361 agtggagggt ctgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      421 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      541 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      601 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      661 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      721 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      781 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      841 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      901 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      961 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1021 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1081 tacaagggcg gctctggcgg gtcagggggg agtaaagtag atgtgattga tttaaccatc
     1141 gagtcgagct ctgacgaaga agaggaccca ccggcgaagc gtgggtccgc tggctcagcg
     1201 ggatctgcgg gaagtgctgg aagcgcgggt tccgctgggt cggcgggttc agccggttcg
     1261 gcggggagtg ccgggtcagc aggttcagcc agtcagaccc aatccccccg caaagtgatc
     1321 aagatggagt tcagaggaag gatccgaaaa cctgtatttc ag
//
