LOCUS       sequence-339778-        7363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses arrestin receiver construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Cho KF, Gillespie SM, Kalogriopoulos NA, Quezada MA, Jacko M, Monje 
            M, Ting AY
  TITLE     A light-gated transcriptional recorder for detecting cell-cell 
            contacts.
  JOURNAL   Elife. 2022 Mar 21;11:e70881. doi: 10.7554/eLife.70881.
  PUBMED    35311648
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-21"; volume: "11:e70881. doi"; pages: " 
            10.7554/eLife.70881"
COMMENT     Sequence Label: pCDNA3 CMV myc-Arrestin-TEVp
FEATURES             Location/Qualifiers
     source          1..7363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..27)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16..32
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(40..46,47..64,65..70))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    85..106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(223..240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(394..982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(474..493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1153..1944,1945..2013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1776..1795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2014..2118)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2193..2212)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2384..2763
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2764..2967
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2918..2938
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3012..3031
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3012..3030
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3055..3084
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(5065..5083)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5066..5083)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5103..5120)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5109..5333
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5379..5807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5466..5485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5676..5697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5816..5836)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5821..6150
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6001..6136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6063..6082
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6217..7011
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6271..6290)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6881..6900
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7185..7306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7222..7241)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7276..7295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(join(7355..7363,1..8))
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(7355..7363,1..8))
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
       61 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      121 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      181 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      241 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      301 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acaaaccccc
      661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1081 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1141 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1201 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1261 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1321 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1381 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1441 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1501 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1561 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1621 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1681 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1741 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1801 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1861 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1921 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1981 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2041 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2101 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     2161 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     2221 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     2281 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     2341 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     2401 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2461 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2521 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2581 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2641 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2701 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2761 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2821 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2881 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2941 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     3001 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttgcggccg catggaacaa
     3061 aagcttattt ctgaagagga cttgggctcg ggcgggagta ccggtggtag tgggagcggt
     3121 ggtgaaaaac ccgggaccag ggtcttcaag aagtcgagcc ctaactgcaa gctcaccgtg
     3181 tacttgggca agcgtgactt tgtggatcac ttggacaaag tggatcctgt cgatggtgtg
     3241 gtgcttgtgg atcctgacta cttgaaggac cggaaagtgt ttgtgaccct cacctgtgcc
     3301 ttccgctatg gccgagaaga cctggatgta ctgggcctgt ctttccgcaa agatctgttc
     3361 atcgccacct accaggcctt cccccccatg cccaacccac ctcggccccc cacccgccta
     3421 caggaccgac tgctgaagaa gttgggccag cacgcccacc cctttttttt cacaataccc
     3481 cagaatttgc cttgctccgt cacactgcag ccaggaccgg aggacacagg gaaggcctgt
     3541 ggagtagact ttgagattcg agccttctgt gccaaatcta tagaagaaaa aagccacaaa
     3601 aggaactccg tgcggcttat catcagaaag gtacagtttg ctcctgagac acccggcccc
     3661 cagccatcag ctgaaaccac acgccacttc ctcatgtctg accggaggtc cctgcaccta
     3721 gaggcttccc tggacaaaga gctgtactac catggggaac ccctcaatgt caacgtccac
     3781 gtcaccaaca attctgccaa aaccgtcaag aagatcagag tgtctgtgag acagtatgcc
     3841 gacatttgcc tcttcagcac cgcgcagtac aagtgtcctg tggctcagct tgaacaagat
     3901 gaccaggtgt ctcccagttc cacattctgc aaggtgtaca ccataacccc gctgctcagt
     3961 gacaaccgag agaagcgtgg ccttgccctt gatgggcaac tcaagcacga agacaccaac
     4021 ctggcttcca gcaccattgt gaaggaggga gccaacaagg aggtgctggg aatcctagta
     4081 tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg gggatgtctc cgtggagcta
     4141 cctttcgtcc taatgcaccc caagccccac gaccacatca cccttccccg accccagtca
     4201 gccccccggg aaatagacat ccctgtggat accaacctca ttgaattcga taccaactat
     4261 gccacagacg acgacatcgt gtttgaggac tttgcgaggc ttcggctgaa ggggatgaag
     4321 gatgacgact gtgatgacca gttctgcggt ggaagtggat caggcagcgg tggatctgga
     4381 gaaagcttgt ttaagggacc acgtgattac aacccgatat cgagcaccat ttgtcatttg
     4441 acgaatgaat ctgatgggca cacaacatcg ttgtatggta ttggatttgg tcccttcatc
     4501 attacaaaca agcacttgtt tagaagaaat aatggaacac tgttggtcca atcactacat
     4561 ggtgtattca aggtcaagaa caccacgact ttgcaacaac acctcattga tgggagggac
     4621 atgataatta ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct gaaatttaga
     4681 gagccacaaa gggaagagcg catatgtctt gtgacaacca acttccaaac taagagcatg
     4741 tctagcatgg tgtcagacac tagttgcaca ttcccttcat ctgatggcat attctggaag
     4801 cattggattc aaaccaagga tgggcagtgt ggcagtccat tagtatcaac tagagatggg
     4861 ttcattgttg gtatacactc agcatcgaat ttcaccaaca caaacaatta tttcacaagc
     4921 gtgccgaaaa acttcatgga attgttgaca aatcaggagg cgcagcagtg ggttagtggt
     4981 tggcgattaa atgctgactc agtattgtgg ggtggccata aagttttcat ggtgtaactc
     5041 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     5101 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5161 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5221 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5281 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5341 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5401 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5461 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5521 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5581 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5641 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5701 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5761 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5821 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5881 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5941 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6001 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6061 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6121 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6181 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6241 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6301 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6361 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6421 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6481 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6541 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6601 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6661 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6721 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6781 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6841 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6901 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6961 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     7021 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7081 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7141 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7201 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7261 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7321 cgtcgacctc tagctagagc ttggcgtaat catggtcata gct
//
