LOCUS       sequence-339780-       10670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CCR6 eLOV receiver construct in DIPG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Cho KF, Gillespie SM, Kalogriopoulos NA, Quezada MA, Jacko M, Monje 
            M, Ting AY
  TITLE     A light-gated transcriptional recorder for detecting cell-cell 
            contacts.
  JOURNAL   Elife. 2022 Mar 21;11:e70881. doi: 10.7554/eLife.70881.
  PUBMED    35311648
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-21"; volume: "11:e70881. doi"; pages: " 
            10.7554/eLife.70881"
COMMENT     Sequence Label: pPB EF1a CCR6-eLOV-TEVcs-Gal4
FEATURES             Location/Qualifiers
     source          1..10670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1367..1484
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1531..1742
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1755..2023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        2048..2110
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             3749..3769
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             3782..3805
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3806..4243
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4244..4588
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    4604..5192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4657..4677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5075..5086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5199..6410
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6247..6266
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6433..7227
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6433..7227
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6487..6506)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7097..7116
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7240..7361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7277..7296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7331..7350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8124..8158
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8649..8665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8649..8665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8662..8684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8673..8689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8697..8703,8704..8721,8722..8727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8742..8763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8880..8897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9051..9639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9131..9150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9810..10601,10602..10670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10433..10452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagaactttt aaaagaaaag
     1381 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1441 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta
     1501 gcctcgaggg atcatccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     1561 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
     1621 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     1681 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     1741 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     1801 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     1861 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     1921 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     1981 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
     2041 cgaattcttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg
     2101 acccgcttaa aagatccagc caccaccggt cgccaccgcc accatgagcg gagagagcat
     2161 gaacttcagt gatgtgttcg attccagtga ggactacttc gtgtccgtca atacatccta
     2221 ctacagcgta gattctgaga tgctgctttg ctccctgcag gaggtgcgcc agttctcccg
     2281 actcttcgtt cccatcgcgt attcactcat ctgcgtcttt ggcctcctgg ggaatatctt
     2341 ggtagtcatc acatttgctt tttataaaaa ggccagatcc atgacagacg tctacctgct
     2401 taatatggcc attgcagaca tcctgttcgt cctgacgctc ccattctggg ctgtgagtca
     2461 cgctaccggg gcctgggtgt tcagtaatgc aacctgcaag ctgttgaagg gaatttatgc
     2521 catcaacttt aattgcggta tgctgctgct gacttgcata agtatggaca gatacatcgc
     2581 catagtacag gcaacaaaat ccttcagact gaggtctcga acgctcccta ggtctaaaat
     2641 catttgtctt gttgtatggg gcttgagcgt gattatttcc agcagtactt tcgtgtttaa
     2701 ccagaagtac aacacgcagg gcagcgatgt gtgtgagccc aagtaccaga cggtatctga
     2761 gcctatcagg tggaagctgt tgatgctggg tttggaattg ctgttcggct tttttatccc
     2821 actcatgttt atgatttttt gttatacctt catcgtgaag accctggtcc aggcacaaaa
     2881 ctctaagcga cacaaggcca tccgggtaat catcgctgtt gtgctggtat ttctggcttg
     2941 ccagatccct cataatatgg tccttctggt aacggcagcc aacctgggca aaatgaatcg
     3001 ctcatgtcag tcagaaaagc ttatcgggta caccaaaacg gttacggagg ttctggcgtt
     3061 cctgcattgt tgccttaacc ctgtgctgta cgcatttata ggtcagaagt ttcgaaacta
     3121 cttcctgaag attctcaaag acctctggtg tgtaagaaga aagtataaaa gtagtggatt
     3181 ttcatgcgca ggacgctact ccgaaaacat ttccagacag accagtgaga cggccgataa
     3241 cgataacgcc tcctccttta ccatgactag tgagctggcc gagaagctgg ccggcctgga
     3301 catcaacggt ggagctagcg gcagccgggc gaccaccctg gaacgtattg aaaagagctt
     3361 tgtgattacc gatccgcgtc tgccggataa cccgattatt tttgtgagcg atagctttct
     3421 gcagctgacc gaatatagcc gtgaagaaat tctgggccgt aactgccgtt ttctgcaggg
     3481 cccggaaacc gatcgtgcga ccgtgcgtaa aattcgtgat gcgattgata accagaccga
     3541 agtgaccgtg cagctgatta actataccaa aagcggcaaa aaattttgga acctgtttca
     3601 tctgcagccg atgcgtgatc agaaaggcga tgtgcagtat tttattggcg tgcagctgga
     3661 tggtaccgaa cgtgtgcgtg atgcggcgga acgtgaagct gtgatgctgg tcaagaaaac
     3721 cgcggaggaa atcgacgaag ccgctaagga aaatctctac ttccagatgg gcggaggatc
     3781 cgactacaag gatgacgacg ataagaagct actgtcttct atcgaacaag catgcgatat
     3841 ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa
     3901 gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca
     3961 tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc
     4021 tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt
     4081 aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc
     4141 agtggagact gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc
     4201 ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcggccaatt ttaatcaaag
     4261 tgggaatatt gctgatagct cattgtcctt cactttcact aacagtagca acggtccgaa
     4321 cctcataaca actcaaacaa attctcaagc gctttcacaa ccaattgcct cctctaacgt
     4381 tcatgataac ttcatgaata atgaaatcac ggctagtaaa attgatgatg gtaataattc
     4441 aaaaccactg tcacctggtt ggacggacca aactgcgtat aacgcgtttg gaatcactac
     4501 agggatgttt aataccacta caatggatga tgtatataac tatctattcg atgatgaaga
     4561 taccccacca aacccaaaaa aagagtaaag cggccgcgtc gacaatcaac ctctggatta
     4621 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4681 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4741 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4801 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4861 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4921 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4981 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     5041 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5101 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5161 gagtcggatc tccctttggg ccgcctcccc gcggatccgg cctccgcgcc gggttttggc
     5221 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     5281 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     5341 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     5401 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     5461 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     5521 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     5581 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     5641 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     5701 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     5761 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     5821 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     5881 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     5941 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     6001 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     6061 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     6121 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     6181 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     6241 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     6301 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     6361 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gctagcggat
     6421 tctagagcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     6481 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     6541 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     6601 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     6661 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6721 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6781 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6841 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6901 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6961 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7021 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7081 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7141 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7201 tatcgccttc ttgacgagtt cttctgagtt aactaactaa acttgtttat tgcagcttat
     7261 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7321 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaattgactc
     7381 aaatgatgtc aattagtcta tcagaagctc atctggtctc ccttccgggg gacaagacat
     7441 ccctgtttaa tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg
     7501 tcaaccaggt tgcagggttt cctgtcctca caggaacgaa gtccctaaag aaacagtggc
     7561 agccaggttt agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt
     7621 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     7681 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     7741 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     7801 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa
     7861 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     7921 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     7981 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     8041 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     8101 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     8161 aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag
     8221 ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc
     8281 ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac
     8341 gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt
     8401 tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag
     8461 cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt
     8521 gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg
     8581 ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg
     8641 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8701 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8761 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8821 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8881 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8941 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9001 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9061 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9241 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9481 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9541 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9661 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9721 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9781 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9841 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9901 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9961 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10021 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10081 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10141 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10201 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10261 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10321 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10381 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10441 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10501 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10561 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10621 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
//
