LOCUS       sequence-339782-       10490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PDIK1L (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10490)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PDIK1L_IDG-K
FEATURES             Location/Qualifiers
     source          1..10490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(113..181,182..973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1144..1732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1633..1652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1886..1903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2020..2041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2056..2061,2062..2079,2080..2086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2094..2110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2099..2121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2118..2134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2118..2134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2153..2173
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2155..2173
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2201..2427
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2428..2608
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2655..2780
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3273..3506
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3691..3735
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4002..4118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4152..4172
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4172..5383
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5220..5239
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5387..5407)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5408..5417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5420..6190
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6197..6238
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6239..6263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7296..7320)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7350..7938
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7403..7423)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7821..7832)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7952..8451
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7987..8010)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8384..8403
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8472..9071
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8472..8491)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8968..8988
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9209..9442
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9514..9635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9551..9570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9605..9624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9675..9810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9830..9849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9831..9849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9859..9876)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9859..9875)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9868..9890)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10017..10472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10104..10123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10314..10335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
       61 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      121 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      181 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      241 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      301 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      361 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      421 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      481 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      541 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      601 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      661 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      721 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      781 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      841 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      901 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      961 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1021 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1081 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1141 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1201 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1261 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1321 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1381 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1441 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1501 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1561 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1621 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1681 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1741 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1801 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1861 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1921 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1981 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     2041 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     2101 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     2161 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     2221 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     2281 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     2341 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     2401 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     2461 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2521 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2581 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2641 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2701 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2761 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2821 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2881 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2941 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     3001 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     3061 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     3121 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     3181 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     3241 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3301 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     3361 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3421 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3481 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     3541 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     3601 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     3661 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     3721 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     3781 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     3841 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     3901 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     3961 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     4021 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     4081 aaagaattac aaaaacaaat tacaaaattc aaaattttat cgtccagtgt ggtggaattc
     4141 tgcagatatc acaactttgt atagaaaagt tggcctccgc gccgggtttt ggcgcctccc
     4201 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     4261 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     4321 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     4381 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     4441 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     4501 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     4561 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     4621 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     4681 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     4741 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     4801 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     4861 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     4921 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     4981 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     5041 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     5101 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     5161 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     5221 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     5281 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     5341 attgtccgct aaattctggc cgtttttggc ttttttgtta gacggccaac ttttgtatac
     5401 aaagtgggcc accatgggaa tcccgctgct gaacgctaaa cagattctgg gacagctgga
     5461 cggcgggagc gtggcagtcc tgcctgtggt cgactccacc aatcagtacc tgctggatcg
     5521 aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg
     5581 aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg
     5641 gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc
     5701 agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta
     5761 tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc
     5821 tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt
     5881 ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct
     5941 ggccgctatg ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct
     6001 ggctccatat ctgtcacggt gggagaagct ggataacttc atcaatagac ccgtgaagct
     6061 gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct
     6121 gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc
     6181 tgccgaaaag gctagcggca agcccatccc caaccccctg ctgggcctgg acagcaccac
     6241 aagtttgtac aaaaaagcag gctccaccat ggtgagtagc cagccaaagt acgatctaat
     6301 acgggaggta ggccgaggta gttacggtgt tgtgtatgaa gcagtcatca gaaagacctc
     6361 tgcacgggtg gcagtgaaga aaattcgatg tcacgcacct gaaaatgttg aactagccct
     6421 tcgtgagttc tgggcactaa gcagtatcaa gagccaacat ccaaatgtga ttcacttgga
     6481 ggaatgcatc ctacaaaagg atgggatggt gcaaaagatg tcccacggct ctaattcttc
     6541 cctttattta cagcttgtag aaacttcatt aaaaggagaa attgcctttg atcccagaag
     6601 cgcctattat ttgtggtttg tgatggattt ttgtgacgga ggagatatga atgagtatct
     6661 gttgtccagg aaacccaatc gtaaaactaa caccagcttc atgcttcagc tgagcagtgc
     6721 cctggctttc ttgcataaaa accagatcat ccaccgagat cttaagcctg ataacatcct
     6781 gatttctcaa accaggttgg ataccagtga cttggaacct accctcaagg tggctgattt
     6841 tggtctaagt aaagtttgtt cagcctctgg gcagaaccca gaagaacctg tcagtgtaaa
     6901 caagtgtttc ctttccacag catgtggaac agatttttac atggctcctg aagtttggga
     6961 aggacattac acagcaaaag ctgacatctt tgctctgggg attatcatct gggcaatgct
     7021 ggaaaggatc acattcatag acacagagac aaagaaggaa ctcttgggga gttatgtaaa
     7081 acaaggaact gagattgtgc ctgttgggga ggcacttctg gaaaatccca aaatggaact
     7141 tctcattcct gtgaagaaaa aatctatgaa tgggcgaatg aaacaactga ttaaggaaat
     7201 gctggctgca aaccctcagg atcgtccaga tgcttttgaa ctagaactca gattagtaca
     7261 aattgcattt aaagatagca gctgggaaac gtaggaccca gctttcttgt acaaagtggt
     7321 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     7381 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7441 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7501 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7561 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7621 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7681 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7741 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7801 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7861 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7921 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
     7981 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     8041 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     8101 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     8161 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     8221 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     8281 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     8341 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     8401 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     8461 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     8521 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     8581 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     8641 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     8701 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     8761 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     8821 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     8881 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     8941 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     9001 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     9061 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     9121 atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     9181 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     9241 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9421 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     9481 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     9541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     9661 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9781 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     9841 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     9901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     9961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
    10021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    10261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    10381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10441 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
//
