LOCUS       sequence-339800-        1566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for glutamate, third generation, variant 857 
            soluble form to express in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Aggarwal A, Liu R, Chen Y, Ralowicz AJ, Bergerson SJ, Tomaska F, 
            Mohar B, Hanson TL, Hasseman JP, Reep D, Tsegaye G, Yao P, Ji X, 
            Kloos M, Walpita D, Patel R, Mohr MA, Tillberg PW, Looger LL, Marvin
            JS, Hoppa MB, Konnerth A, Kleinfeld D, Schreiter ER, Podgorski K
  TITLE     Glutamate indicators with improved activation kinetics and 
            localization for imaging synaptic transmission.
  JOURNAL   Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6.
  PUBMED    37142767
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6."
COMMENT     Sequence Label: pRSET iGluSnFR3 v857
FEATURES             Location/Qualifiers
     source          1..1566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     966..987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1090..1111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggatccgccg caggcagcac gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt
       61 caccgtgact cttcagtgcc tttctcttat tacgacagtc agcacaaagt ggtgggttac
      121 tcgcaggaat actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccggac
      181 ttgcaggtaa aactgattcc gattacttca caaaaccgta ttccactact gcaaaacggc
      241 acttacgatt ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa acaggcggct
      301 ttctctgaca ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg tggcgatatc
      361 aaagattttg ccaacctgaa agacaaagcc gtagtcgtca cttccggcac tacctctgaa
      421 gttttgctca acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa
      481 gatcacggtg actctttccg caccctggaa agcggtcgtg ccgttgcctt tatgatggat
      541 gactcgctgc tggccggtga acgtgcgaaa gcgaagaaac cagacgactg ggaaatcgtc
      601 ggcaagccgc agtctcagga ggcctacggt tgtatgttgc gtaaagatga tccgcagttc
      661 aaaaagctga tggatgacac catcgctcag gtgcagacct ccggtgaagc ggaaaaatgg
      721 tttgataagt ggttcaaaaa tccaattctg gtgaaccaca cggtctatat caccgccgac
      781 aagcagaaga acggcatcaa ggcgaacttc aagatccacc acaacgtgga ggacggcagc
      841 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      901 cccgacaacc actacctgag ctatcagtcc gtgctgagca aagaccccaa cgagaagcgc
      961 gatcacatgg tcctgctgga gttcgtgacc gccaccggga actctctcgg catggacgag
     1021 ctgtacaagg gcggtaccgg agggagcatg agcaagggcg aggagctgtt caccggggtg
     1081 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc
     1141 gagggcgagg gcgatgccac caacggcaag ctaaccctga agttcatctg caccaccggc
     1201 aagctgcccg tgccctggcc caccctcgtg accaccctga gctacggcgt gcagtgcttc
     1261 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1321 tacgtccagg agcgcaccat cagcttcaag gacgacggaa cctacaagac ccgcgccgag
     1381 gtgaagttag agggcgacac cctggtgaac cgcatcgagc taaagggcat cgacttcaag
     1441 gaggacggca acatcctggg gcacaagctg gagtacaact ttaacaaccc cctgaacatg
     1501 aatttcgaac tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaggcactg
     1561 aaataa
//
